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Yoo, Sun Kyun,Kim, Do Man,Donal F. Day 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.4
The influence of process conditions on highly branched glucooligosaccharides production by mixed culture of Leuconostoc mesenteroides ATCC 13146 and Lipomyces starkeyi ATCC 74054 was studied. We divided the batch culture fermentations into two groups according to inoculation method. One-point inoculation was performed by coinoculation of L. mesenteroides and L. starkeyi at the ratio of 10 to 1, and two-point inoculation by L. mesenteroides inoculation first and L. starkeyi inoculation after L. mesenteroides grew to the end of the log phase of growth. Two-point inoculation improved the yield of oligosaccharide by 1.5 to 2.0 fold more than one-point inoculation. In this process, the highest yield of oligosaccharides (48% of theoretical yield) and productivity (0.85 g/l/h) were obtained with starch as an initial substrate for L. starkeyi growth. The estimated composition of the end product consisted of 31.5% oligosaccharides, 17.6% dextran, and 46.5% mannitol.
Yoo, Sun Kyun,Gaugler, Randy,Brey, Christopher W. 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.2
공생 박테리아Photorhabdus sp. strain TF 균체량이 증가함에 따라서 병원성 선충 Heterorhabditis bacteriophora의 Infective Juveniles의 생산이 증가 되었다. 이 공생 박테리아의 성장 최적 조건은 각각 배양 온도 30℃ 그리고 초기 배지 pH 5.5 부터 7.3 사이에서 얻어졌다. 통기 상태에서 박테리아 균체의 생산과 성장이 촉진됨이 밝혀졌다. Photorhabdus sp. strain TF 박테리아의 성장 중 생산된 색소와 배양액의 pH의 변화는 박테리아의 성장 정도 나타내어 액체배양에서 병원성 선충 Heterorhabditis bacteriophora의 접종시기로 표시로서 이용할 수 있을 것이다. The yield of infective juveniles of Heterorhabditis bacteriophora (Tf strain) in vitro monoxenic liquid culture was improved significantly as the amount of symbiont biomass, Photorhabdus sp. strain Tf, increased. To investigate the influence of abiotic factors on the growth and biomass production of Photorhabdus sp. strain Tf, triplicate flask cultures were performed. The optinal temperature and medium pH for the growth of Photorhabdus sp. strain Tf were 30℃ and between pH 5.5~7.3, respectively. Aeration also improved greatly growth and yield of biomass of Photorhabdus sp. strain Tf. Photorhabdus sp. strain Tf in batch fermentation showed growth-associated pattern in terms of pigment production, and the pH of culture medium rose steadily until growth stopped during the fermentation. Both pigment production and culture pH rise would be useful parameters indicating a reliable growth of Photorhabdus sp. strain Tf.
Yoo, Sun Kyun,Brown, Lan,Cohen,Nancy,Gaugler, Randy 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.4
The biological control potential of entomopathogenic nematodes (EPN) can be enhanced by improved culture efficiency. Optimization of the media is a key factor for improving in vitro mass production of entomopathogenic nematodes. This study reports the effect of medium concentration. The medium is a combination of carbohydrates, lipids, proteins, salts, and growth factors, on the growth of Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens. The overall optimal medium concentration for nematode recovery, hermaphrodite size, bacterial mass, infective juveniles (IJs) yield, and doubling time was 84g/l. At this concentration rate, the doubling time of IJs production and the biomass of symbiotic bacteria was 1.6 days and 12.8g/l, respectively. The maximum yield of 2.4×10 exp(5)IJs/ml was attained within a one-generation cycle (eight days). The yield coefficient was 2.8×10 exp(6)IJs/g medium, and the maximum productivity was 3.1×10 exp(7)IJs per day. Medium concentration affected two independent factors, recovery and hermaphrodite size, which in turn influenced the final yield.
Co-Production of Dextran and Mannitol by Leuconostoc mesenteroides
YOO, SUN KYUN,KIM, DOMAN,DONAL F.DAY 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.5
To optimize co-production of dextran and mannitol from sucrose by Leuconostoc mesenterides ATCC 13146, a batch culture fermentation was conducted by using various concentrations of sucrose and initial culture pHs. The production of dextran and mannitol showed a growth-associated pattern. The highest yields of both dextran and mannitol were obtained at pH 6.0 and 10% (w/v) sucrose. They could be easily separated by using alcohol fractionation. Maximum yields of dextran and mannitol were 0.45 and 0.35 of the consumed sucrose, respectively. Overall productivities of dextran and mannitol were 1.47 and 0.37 g/l/h, respectively.