Expression of granulocyte colony-stimulating factor 3 receptor in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain

Zhang, Enji,Lee, Sunyeul,Yi, Min-Hee,Nan, Yongshan,Xu, Yinshi,Shin, Nara,Ko, Youngkwon,Lee, Young Ho,Lee, Wonhyung,Kim, Dong Woon SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol.16 No.2

<P>In previous studies that have profiled gene expression in patients with complex regional pain syndrome (CRPS), the expression of granulocyte colony-stimulating factor 3 receptor (G-CSFR) was elevated, as were a number of pain-associated genes. The present study determined the expression of G-CSFR and the mechanisms by which it may affect hypersensitivity, focusing on the signal transducer and activator of transcription 3 (STAT3)/transient receptor potential cation channel subfamily V 1 (TRPV1) signaling pathway in particular, which is an important mediator of pain. Following L5 spinal nerve ligation (SNL) surgery, the protein and mRNA levels of G-CSFR increased in the ipsilateral spinal dorsal horn when compared with the sham and/or contralateral control. Double immunofluorescence further demonstrated that G-CSFR colocalized with TRPV1 and phosphorylated STAT in the neurons of the spinal dorsal horn. G-CSF treatment led to an increase in G-CSFR and TRPV1 expression and phosphorylation of STAT3. These results indicate that G-CSF-induced G-CSFR expression may activate TRPV1 by promoting phosphorylation of STAT3. Collectively, the results suggest, for the first time, that the expression of G-CSFR in neurons following peripheral nerve injury may be involved in the induction and maintenance of neuropathic pain through the STAT3 and TRPV1 signaling pathway.</P>

Investigation on damage development of AP1000 nuclear power plant in strong ground motions with numerical simulation

Wanruo Chen,Yongshan Zhang,Dayang Wang,Chengqing Wu 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.6

Seismic safety is considered to be one of the key design objectives of AP1000 nuclear power plant (NPP)in strong earthquakes. Dynamic behavior, damage development and aggravation effect are studied in thisstudy for the three main components of AP1000 NPP, namely reinforced concrete shield building (RCSB),steel vessel containment (SVC) and reinforced concrete auxiliary building (RCAB). Characteristicsincluding nonlinear concrete tension and compressive constitutions with plastic damage are employedto establish the numerical model, which is further validated by existing studies. The author investigatesthree earthquakes and eight input levels with the maximum magnitude of 2.4 g and the results showthat the concrete material of both RCSB and RCAB have suffered serious damage in intense earthquakes. Considering RCAB in the whole NPP, significant damage aggravation effect can be detected, which ismainly concentrated at the upper intersection between RCSB and RCAB. SVC and reinforcing bardemonstrate excellent seismic performance with no obvious damage.

Performance Evaluation and Shear Resistance of Modular Prefabricated Two-Side Connected Composite Shear Walls

Can Mei,Zhendong Zhao,Yongshan Zhang,Dayang Wang,Chengqing Wu 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.8

This paper investigates the impact of configuration parameters on the seismic performance of the modular prefabricated two-side connected composite shear wall. Firstly, the finite element model of the modular prefabricated two-side connected composite shear wall was established and validated by Xu’s experimental results. Secondly, three aspects of parameter investigationswere discussed in detail based on the validated numerical technology, namely the design parameter of ISP, design parameter of the stud, and design parameter of reinforced concrete faceplate (RCF). Then, the computation formula of shear capacity is deduced on the basis of the finite element model for reference to structural design. The results of parameter analysis displayed that the seismic performance of the modular prefabricated two-side connected composite shear wall has excellent seismic performance with the array studs of the length-diameter ratio of 4 which the center distance of 150 mm, and RCF with the thickness of 75 mm. A satisfactory but unadventurous estimation of the shear capacity of a modular prefabricated two-side connected composite shear wall is supplied by the advised technique.

Parametric Study on Cyclic Behavior of Modular Assembly Composite Walls with Connection to Boundary Beam

Tong Ou,Dayang Wang,Yongshan Zhang,Hao Li 한국강구조학회 2021 International Journal of Steel Structures Vol.21 No.5

This study focuses on the investigation of seismic performance of modular assembly composite shear wall (MACW) system, which is connected to boundary beams only. The fi nite element method is employed to establish the numerical model of the MACW system and experimental data is adopted to validate the simulation. To clearly show the seismic performance of the system, seven design parameters with 36 computational cases, including height-to-span and height-to-thickness ratios of steel plate, bolt spacing, spacing between the concrete plate and connection steel plate, thicknesses of the connection steel plate and concrete plate, and the number of composite wall modules, are discussed on mechanical property and energy dissipation capacity in details. The results show that ratios of height-to-span and height-to-thickness have a signifi cant infl uence on mechanical bearing and energy dissipation properties in comparison to the other fi ve parameters. Combining systematic seismic behaviors, such as hysteresis property, out-of-plane displacement, and equivalent damping ratio, the optimal values of the above seven parameters are recommended to be 2, 600, 100, 30, 8, 50, and 2 mm respectively. Stiff ness ratios between the post-yield and initial stiff ness of all the 36 models are mainly in the range of (2.6%, 5.5%).

Seismic response characteristics of base-isolated AP1000 nuclear shield building subjected to beyonddesign basis earthquake shaking

Dayang Wang,Chuli Zhuang,Yongshan Zhang 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.1

Because of the design and construction requirements, the nuclear structures need to maintain thestructural integrity under both design state and extreme earthquake shaking. The base-isolation technologycan significantly reduce the damages of structures under extreme earthquake events, andeffectively protect the safeties of structures and internal equipment. This study proposes a base-isolationdesign for the AP1000 nuclear shield building on considering the performance requirements of theseismic isolation systems and devices of shield building. The seismic responses of isolated and nonisolatedshield buildings subjected to design basis earthquake (DBE) shaking and beyond-design basisearthquake (BDBE) shaking are analyzed, and three different strategies for controlling the displacementssubjected to BDBE shaking are performed. By comparing with nonisolated shield buildings, the flooracceleration spectra of isolated shield buildings, relative displacement, and base shear force are significantlyreduced in high-frequency region. The results demonstrate that the base-isolation technology isan effective approach to maintain the structural integrity which subjected to both DBE and BDBE shaking. A displacement control design for isolation layers subjected to BDBE shaking, which adopts fluiddampers for controlling the horizontal displacement of isolation layer is developed. The effectiveness ofthis simple method is verified through numerical analysis

Research on the impact effect of AP1000 shield building subjected to large commercial aircraft

Wang, Xiuqing,Wang, Dayang,Zhang, Yongshan,Wu, Chenqing Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.5

This study addresses the numerical simulation of the shield building of an AP1000 nuclear power plant (NPP) subjected to a large commercial aircraft impact. First, a simplified finite element model (F.E. model) of the large commercial Boeing 737 MAX 8 aircraft is established. The F.E. model of the AP1000 shield building is constructed, which is a reasonably simplified reinforced concrete structure. The effectiveness of both F.E. models is verified by the classical Riera method and the impact test of a 1/7.5 scaled GE-J79 engine model. Then, based on the verified F.E. models, the entire impact process of the aircraft on the shield building is simulated by the missile-target interaction method (coupled method) and by the ANSYS/LS-DYNA software, which is at different initial impact velocities and impact heights. Finally, the laws and characteristics of the aircraft impact force, residual velocity, kinetic energy, concrete damage, axial reinforcement stress, and perforated size are analyzed in detail. The results show that all of them increase with the addition to the initial impact velocity. The first four are not very sensitive to the impact height. The engine impact mainly contributes to the peak impact force, and the peak impact force is six times higher than that in the first stage. With increasing initial impact velocity, the maximum aircraft impact force rises linearly. The range of the tension and pressure of the reinforcement axial stress changes with the impact height. The perforated size increases with increasing impact height. The radial perforation area is almost insensitive to the initial impact velocity and impact height. The research of this study can provide help for engineers in designing AP1000 shield buildings.

Analysis on the temperature dependent electrical properties of graphene/ Al–ZnO Schottky contact

Yapeng Li,Yingfeng Li,Jianhua Zhang,Xiangyu Zou,Yongshan Wang 한국물리학회 2019 Current Applied Physics Vol.19 No.10

The electrical characteristics of the graphene/Al–ZnO schottky contact with the temperature of 180 K–300 K have been discussed in detail. Based on the TE model, the barrier height increased and the ideal factor decreased with the increasing of temperature from 180 K to 300 K, which can be interpreted by the lateral inhomogeneities of schottky barrier height. Combined with the single gauss distribution function of barrier height, the lateral inhomogeneities distribution of barrier height at the interface was confirmed. By DLTS measurement, one electron trap centers were observed at the interface.

Mechanical Behavior of Nine Tree-Pool Joints Between Large Trees and Buildings

Wang Dayang,Liu Mingqi,Ou Tong,Zhang Yongshan 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8

Nine types of tree-pool joints designed to plant large trees on building structures were proposed in this study. Solid finite elementmodel considering plastic damage constitutive relationship for the tree-pool joints were built using ABAQUS software platform. Based on comparative analysis of six different mesh generation methods of the 3D tree-pool joint model, an optimized meshgeneration method was determined and verified considering both the calculation time and accuracy. The mechanical behavior of thenine tree-pool joints, such as peak tension/compression stress, hysteretic energy dissipation performance, plastic damageperformance and corresponding crack development process, were studied in detail under horizontal and vertical loads. The resultsshow that the tree-pool joints with steel strengthened form and composite strengthened form have superior horizontal and verticalbearing capacity. Similarly, they also have stable hysteretic energy dissipation performance, minimal plastic damage and crackdevelopment relatively in vertical ultimate load. However, the tree-pool joints with other strengthened forms, such as forms of innerbeamand inner-beam combined with ring bracket, show poor mechanical properties. Although there is a certain degree ofperformance improvement for these tree-pool joints relative to non-strengthened joints, narrow hysteretic curves, apparent strength/stiffness degradation characteristics, extensive material damage and crack development can be found. Results of this paper do shinesome lights on how to design reasonable and reliable tree-pool joints in building structures.

Experimental Study on Mechanical Properties of Continuous Welding Stainless Steel Roof System under Temperature Effect

Mingming Wang,Tong Ou,Zhiyong Xin,Dayang Wang,Yongshan Zhang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2

Stainless steel is used as a new generation of long-span metal roof systems with continuous welding technology, which exhibits many unknown behaviors during temperature action. This study focuses on the temperature effect of a continuous welded stainless steel roof (CWSSR) system and analyzes the stress distribution of plate rib (PR), plate surface (PS), and support (SU), and the horizontal and vertical displacement. Furthermore, the thermal action of the CWSSR system is comprehensively analyzed considering different loading rates, constant temperature time, and the cycle number. Research results show that the stress concentration and thermal expansion of the CWSSR system are obvious, and fatigue damage occurs under long-term temperature action. The responses of the CWSSR system are greatly influenced by the loading rate and the cycle number but are less influenced by the constant temperature time. Loading rate and the cycle number mainly affect the plate surface stress and vertical displacement. The plate surface stress and vertical displacement peak increased by 34.6% and 29.6% with the loading rate, and changed by 32.4% and 42.5% with the cycle number. Cyclic loading reduces support tensile capacity by 4.4%. The research results can provide reference for the design and application of temperature field resistance of the CWSSR system.

Wind resistance performance of a continuous welding stainless steel roof under static ultimate wind loading with testing and simulation methods

DaYang Wang,Zhendong Zhao,Tong Ou,Zhiyong Xin,Mingming Wang,Yongshan Zhang 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.32 No.1

Ultrapure ferritic stainless steel provides a new generation of long-span metal roof systems with continuous welding technology, which exhibits many unknown behaviors during wind excitation. This study focuses on the wind-resistant capacity of a new continuous welding stainless steel roof (CWSSR) system. Full-scale testing on the welding joints and the CWSSR system is performed under uniaxial tension and static ultimate wind uplift loadings, respectively. A finite element model is developed with mesh refinement optimization and is further validated with the testing results, which provides a reliable way of investigating the parameter effect on the wind-induced structural responses, namely, the width and thickness of the roof sheeting and welding height. Research results show that the CWSSR system has predominant wind-resistant performance and can bear an ultimate wind uplift loading of 10.4 kPa without observable failures. The welding joints achieve equivalent mechanical behaviors as those of base material is produced with the current of 65 A. Independent structural responses can be found for the roof sheeting of the CWSSR system, and the maximum displacement appears at the middle of the roof sheeting, while the maximum stress appears at the connection supports between the roof sheeting with a significant stress concentration effect. The responses of the CWSSR system are greatly influenced by the width and thickness of the roof sheeting but are less influenced by the welding height. Ultrapure ferritic stainless steel provides a new generation of long-span metal roof systems with continuous welding technology, which exhibits many unknown behaviors during wind excitation. This study focuses on the wind-resistant capacity of a new continuous welding stainless steel roof (CWSSR) system. Full-scale testing on the welding joints and the CWSSR system is performed under uniaxial tension and static ultimate wind uplift loadings, respectively. A finite element model is developed with mesh refinement optimization and is further validated with the testing results, which provides a reliable way of investigating the parameter effect on the wind-induced structural responses, namely, the width and thickness of the roof sheeting and welding height. Research results show that the CWSSR system has predominant wind-resistant performance and can bear an ultimate wind uplift loading of 10.4 kPa without observable failures. The welding joints achieve equivalent mechanical behaviors as those of base material is produced with the current of 65 A. Independent structural responses can be found for the roof sheeting of the CWSSR system, and the maximum displacement appears at the middle of the roof sheeting, while the maximum stress appears at the connection supports between the roof sheeting with a significant stress concentration effect. The responses of the CWSSR system are greatly influenced by the width and thickness of the roof sheeting but are less influenced by the welding height