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- 저자 : Yongfu Qiu (검색결과 2 건)
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Yongfu Qiu,Xiaozhen Jiao,Dehui Hu,Fang Liu,Fengkuan Huang,Rongbai Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
A total of 320 ESTs (Expressed Sequence Tags, 12.8%) of rice planthoppers were identified to carry SSR (Simple Sequence Repeat) motifs from2491 ESTs downloaded frompublic database, and 397 pairs of brown planthopper (BPH; Nilaparvata lugens Stål) and 25 pairs ofwhite-backed planthopper (WBPH; Sogatella furcifera) (Hemiptera: Delphacidae) EST-SSR were detected based on the motifs. The dinucleotide repeat was the dominant type with repeat motif AG/GA/TC/CT; and the trinucleotide repeat was the style of AAG/AGA/GAA/TTC/TCT/CTT among the BPH repeat motifs. A total of 20 EST-SSRs (16 developed in the present study and four fromprevious studies) were applied to survey the genetic diversity of the 15 rice planthoppers. Consequently, 18 pairs of primer amplifies clear, dominant and polymorphic gel bands among the collected planthoppers population; one ESTSSR amplified a single gel band; and one had no result. Furthermore, a total of 227 bands were produced by the selected 19 pairs of primers, of which 207 bands (91.2%) were polymorphic among the test insect populations. Genetic diversity analysis suggested that the BPH populations from Cuu Long, Thailand and Ho Chi Minh were clustered, respectively; and Hue and Long Xuyen were composed into one class; the others were clustered together when the similar coefficient was 0.76. The results show that it is an effective and available approach to develop SSR markers based on ESTs in rice planthoppers; and also the developed EST-SSR markers could detect the genetic diversity of the planthoppers population, which would be beneficial for rice planthoppers control strategy. ©
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Lilong Liu,Baoxiang Qin,Yunzhen Li,Minyi Wei,Zishuai Wu,Fang Liu,Yongfu Qiu,Jijing Luo,Rongbai Li 한국유전학회 2017 Genes & Genomics Vol.39 No.11
Awn is one of important traits during rice domestication. To understand the development of rice awn and the roles it played in rice domestication, we preliminary mapped a major QTL An-3 for awn development using chromosome segment substitution line CSSL138 developed by introgressed genomic fragments of long-awned Guangxi common wild rice (GXCWR, Oryza rufipogon Griff.) into genetic background of short-awned indica cultivar 93–11. An-3 was then fine mapped to a 7-kb region of chromosome 8. An epidermal patterning factor-like protein gene was identified as the single candidate gene corresponding to this QTL. An-3 was showed to be an allele of RAE2 and GAD1, and negatively regulated 1000-grains weight, grain length, and length–width ratio. Comparing with the coding sequences of An-3 from CSSL138, a 2- and 4-bp frameshift deletions in the second exon were identified in 93–11 and Nipponbare, respectively. Taken together, our results provide valuable natural variation in the alleles of An-3 between common wild rice and cultivated rice, which will be helpful in clarifying the mechanism of awn development and promoting the application of an-3 in genetic improvement of rice yield traits.
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