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- 저자 : Yingxue Hu (검색결과 2 건)
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Yun She,Chunwei Zhang,Mohammad Azis Mahardika,Anindityo Patmonoaji,Yingxue Hu,Shintaro Matsushita,Tetsuya Suekane 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.98 No.-
This study presented a pore-scale investigation on the dynamic displacement of oil subjected to ex-situand in-situ surfactantflooding using X-ray microtomography. The ex-situ surfactant solution was preprepared,whereas the in-situ surfactant was generated directly with the chemical reaction inside porousmedia. The results showed that oil removal from the pore spaces relied on the combination of piston-like,pore-bodyfilling and a weak emulsification mechanism in the ex-situ system, whereas the in-situ systemwas dominated by a strong emulsification process. The threshold capillary pressure was decreasedsignificantly owing to interfacial tension reduction and wettability alteration, which enhanced the oilcluster mobilization. Therefore, the in-situ system produced the highest oil recovery efficiency of 86.9%compared with ex-situ system and waterflooding. The emulsifying ability was evaluated through oilcluster size distributions and their dynamic evolution. The networks or branches disappeared andemulsified into small oil ganglia and singlets, thereby leading to a significant decrease in the averageequivalent diameter. Finally, strong emulsification in the in-situ system was attributed to quick andprecise surfactant aggregation at the oil–water interface that induced blebbing, whereas the ex-situ casewas dominated by slow surfactant transport relying on advection and diffusion in theflow mainstream.
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Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells
Lei An,Shengnan Liu,Wei Zhang,Yamei Zhang,Yingxue Huang,Xinde Hu,Shu-lin Chen,Shanting Zhao 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.1
Molecular mechanisms underlying the effects of Fyn on cellmorphology, pseudopodium movement, and cell migrationwere investigated. The Fyn gene was subcloned into pEGFP-N1to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO)cells were transfected with pEGFP-N1-Fyn. The expression ofFyn mRNA and proteins was monitored by reversetranscription-PCR and Western blotting. Additionally,transfected cells were stained with 4´,6-diamidino-2-phenylindole and a series of time-lapse images was taken. Sequences of the recombinant plasmids pMD18-T-Fyn andpEGFP-N1-Fyn were confirmed by sequence identificationusing National Center for Biotechnology Information in USA,and Fyn expression was detected by RT-PCR and Westernblotting. The morphology of CHO cells transfected with therecombinant vector was significantly altered. Fyn expressioninduced filopodia and lamellipodia formation. Based on theseresults, we concluded that overexpression of mouse Fyn inducesthe formation of filopodia and lamellipodia in CHO cells, andpromotes cell movement.
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