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Ying Wang,Bu-li Cui,Dong-sheng Li,Ya-xuan Wang,Wan-xin Yu,He-hua Zong 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.2
A prerequisite for using isotopic techniques to study the regional water cycle of a mountainous area is to examine the stable isotopic composition of precipitation. These findings are of great significance for an in-depth understanding of water cycle processes. In this study, each precipitation event was sampled and used to investigate the characteristics of stable hydrogen and oxygen isotopes in precipitation over the Jiaolai Plain and its surrounding areas. NCEP/NCAR data was used for the wind speed and direction, relative humidity, and precipitable amount in the study area during the sampling period. The water vapor sources of the precipitation over the plain were revealed through a comparative analysis of seasonal variations in precipitation isotopes between Global Network of Isotopes in Precipitation stations located along different vapor transport paths. The results showed that the local meteoric water line was δ2H = 6.38 δ18O + 0.72, with a gradient of less than 8. This indicates that the precipitation process was affected by non-equilibrium evaporation occurring when the drops fell below the cloud base. Temperature and amount effects were observed in the δ18O of the precipitation, although the altitude effect was not significant. The water vapor source of the precipitation was predominantly controlled by the East Asian Monsoon from June to September, with the primary source being evaporation from the adjacent Pacific Ocean. The plain was controlled by the Westerlies from October through May, with the predominant vapor source being local evaporation. Water vapor from the polar region had a minimal impact. These findings can serve as the basis for studying surface water–groundwater–seawater transformations.
Pan Ying-Hua,Nong Bao-Xuan,Chen Lei,Yang Xing-Hai,Xia Xiu-Zhong,Zhang Zong-Qiong,Qing Dong-Jin,Gao Ju,Huang Cheng-Cui,Li Dan-Ting,Deng Guo-Fu 한국유전학회 2023 Genes & Genomics Vol.45 No.7
Background Cold damage stress significantly affects rice growth (germination and seedling) and causes serious losses in yield in temperate and high-altitude areas around the globe. Objective This study aimed to explore the cold tolerance (CT) locus of rice and create new cold-tolerant germplasm. We constructed a chromosome segment substitution line (CSSL) with strong CT and fine mapped quantitative trait loci (QTLs) associated with CT by performing the whole-genome resequencing of CSSL with phenotypes under cold treatment. Methods A chromosome CSSL, including 271 lines from a cross between the cold-tolerant wild rice Y11 (Oryza rufipogon Griff.) and the cold-sensitive rice variety GH998, was developed to map QTLs conferring CT at the germination stage. The whole-genome resequencing was performed on CSSL for mapping QTLs of associated with CT at the germination stage. Results A high-density linkage map of the CSSLs was developed using the whole-genome resequencing of 1484 bins. The QTL analysis using 615,466 single-nucleotide polymorphisms (SNPs) led to the identification of 2 QTLs related to germination rate at low-temperature on chromosome 8 (qCTG-8) and chromosome 11 (qCTG-11). The qCTG-8 and qCTG-11 explained 14.55% and 14.31% of the total phenotypic variation, respectively. We narrowed down qCTG-8 and qCTG-11 to 195.5 and 78.83-kb regions, respectively. The expression patterns of important candidate genes in different tissues, and of RNA-sequencing (RNA-seq) in CSSLs, were identified based on gene sequences in qCTG-8 and qCTG-11 cold-induced expression analysis. LOC_Os08g01120 and LOC_Os08g01390 were identified as candidate genes in qCTG-8, and LOC_Os11g32880 was identified as a candidate gene in qCTG-11. Conclusions This study demonstrated a general method that could be used to identify useful loci and genes in wild rice and aid in the future cloning of candidate genes of qCTG-8 and qCTG-11. The CSSLs with strong CT were supported for breeding cold-tolerant rice varieties.
중국 장백산의 습지성 진달래과 식물 분포와 생태적 특성
金英花 ( Ying Hua Jin ),玄永男 ( Yong Nan Xuan ),이성제 ( Sung Je Lee ),안영희 ( Young Hee Ahn ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.11
This study was conducted for clarifying distribution and characteristics of marshy Ericaceae plants on Mt. Changbai in China. The marshy Ericaceae plants in wetland of Mt. Changbai were investigated on about 1400 m altitude. This region is various developmental wetlands and vegetation mixture of needle-leaf trees and broad-leaf trees. This region also is start point of the coniferous forest zone in Mt. Changbai. As results of these researches, L. palustre var. diversipilosum and L. palustre var. decumbens populations were investigated in Larix olgensis var. amurensis community. R. lapponicum subsp. parvifolium var. parvifolium community appeared as a typical community. Environmental conditions of L. palustre var. diversipilosum and L. palustre var. decumbens populations`` habitats are more soil humidity and shade condition. R. lapponicum subsp. parvifolium var. parvifolium community habitat, however, has more deposits and humidity condition. Therefore when the plants were cultivated, we should recognize the growth condition such as maintenance of humidity and control deposits. The flora of marshy Ericaceae species habitats was investigated as 28 families, 49 genera, 45 species, 14 variety, 1 forma, 1 sub-species, and total 61 taxa.
Liu, Ying,Zhou, Yan-Dong,Xiao, Yu-Li,Li, Ming-Hua,Wang, Yu,Kan, Xuan,Li, Qiu-Ying,Lu, Jian-Guang,Jin, De-Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Background: To examine the expression of cysteine-rich 61 (Cyr61/CCN1) protein in laryngeal squamouscell carcinoma (LSCC) tissues, and its relationship with the tumor epithelial-mesenchymal transition (EMT), invasion, metastasis, and prognosis. Materials and Methods: Immunohistochemistry was used to detect the expressions of Cyr61, Vimentin (Vim), and E-cadherin (E-cad) in 88 cases of LSCC tissues and 30 cases of tumor-adjacent normal tissues. Vim and E-cad were used as mesenchymal and epithelial markers, respectively, to determine the relationship between Cyr61 expression and the EMT of LSCC cells. In addition, clinical and histopathological data were combined to analyze the relationship between the positive-expression rates of Cyr61, Vim and E-cad and LSCC invasion, metastasis and prognosis. Results: In LSCC tissues, Vim expression rate was significantly higher than that of the tumor-adjacent tissues, whereas E-cad expression rate was significantly lower than that of the tumor-adjacent tissues. The Vim expression rate was significantly higher in stages T3 and T4 than in stages T1 and T2 LSCC tissues, whereas E-cad expression rate was significantly lower in stages T3 and T4 than in stages T1 and T2 LSCC tissues. Compared to the group without lymph node metastasis, the Vim expression rate was significantly higher and the E-cad expression rate was significantly lower in the group with lymph node metastasis. The expression rate of Cyr61 was significantly higher in LSCC tissues than in the tumor-adjacent normal tissues. In addition, the Cyr61 expression rate was higher in stages T3 and T4 than in stages T1 and T2 LSCC, and higher in the group with lymph node metastasis than in the group without lymph node metastasis. The Vim expression rate was significantly higher in the Cyr61 positive group than in the Cyr61 negative group, whereas the E-cad expression rate was significantly higher in the Cyr61 negative group than in the Cyr61 positive group. Survival analysis indicated that survival rates of Cyr61 positive, Vim positive and E-cad negative groups were significantly lower than that of Cyr61 negative, Vim negative and E-cad positive groups, respectively. Conclusions: Cyr61 expression is closely associated with LSCC invasion and lymph node metastasis. Overexpression of Cyr61 may induce EMT and therefore leads to LSCC invasion and metastasis and poor prognosis. Cyr61 may become a new maker for clinical prediction of LSCC invasion and metastasis and a new target for LSCC treatment.
<i>Acanthamoeba</i>: Keratopathogenicity of isolates from domestic tap water in Korea
Jeong, Hae Jin,Lee, Sun Joo,Kim, Jeong Hwan,Xuan, Ying Hua,Lee, Keun Hee,Park, Sang Kyun,Choi, Sun Hee,Chung, Dong Il,Kong, Hyun Hee,Ock, Mee Sun,Yu, Hak Sun Elsevier 2007 Experimental parasitology Vol.117 No.4
<P><B>Abstract</B></P><P>In a previous study, we reported on the contamination rate of free living amoeba, including <I>Acanthamoeba,</I> isolated from contact lens storage cases (CLSC) and domestic tap water in Korea. In an effort to evaluate the potential kerato-pathogenicity of 5 isolates from CLSC and 17 isolates from domestic tap water, we have conducted an investigation into the morphological features, mitochondrial DNA (mtDNA) restriction fragment length polymorphism (RFLP) phenotypes, 18S rDNA sequences, and drug sensitivities of these isolates, and have compared the results with those of 20 amoebic keratitis (AK) isolates from Korea, as well as 14 reference strains. Cysts from 22 isolates obtained from CLSC and domestic tap water showed typical characteristics of morphological group 2. A total of three and five mtDNA RFLP patterns generated by EcoRI were found in 5 of the isolates from CLSC and 17 of the isolates from domestic tap water, respectively. The mtDNA RFLP patterns of four of the five isolates from the CLSC were found to be identical to those of the isolates from domestic tap water of students who had contaminated CLSC. The majority had mtDNA RFLP patterns identical to those of AK isolates in Korea. The results of 18S rDNA sequencing analysis were also shown to coincide with the results of mtDNA RFLP analysis. KA/WP12 was determined to be profoundly sensitive to chlorhexidine (MCC; 6.25μg/ml), and KAWP2 was the most sensitive strain to polyhexamethylene biguanide (PHMB) (MCC; 4.69μg/ml). Some difference in the cytopathic effects of isolates against human corneal epithelial cells was observed according to their mtDNA genotypes. In conclusion, domestic tap water may constitute a source of <I>Acanthamoeba</I> contamination of CLSC, and most isolates from CLSC and domestic tap water appear to be potentially keratopathogenic.</P>