Hemocyte Changes after the Extirpation of the Hemopoietic Organ-wing Disc Complexes in the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Zhou, Qing-Xiang,Shen, Xing-Jia,Yi, Yong-Zhu,Xia, Ai-Hua,Zhang, Zhi-Fang Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.13 No.2

We successfully extirpated all four hemopoietic organ-wing disc complexes of the fifth instar larvae of Bombyx mori, and found that most of the treated silkworms could still develop into the moths. We investigated the changes of the circulating hemocytes and evaluated the effects of extirpation on the hemopoiesis. The results showed that proliferation of circulating hemocytes was sufficient to allow development of the silkworms which complexes were totally extirpated. We also found that hemopoietic organ-wing disc complexes extirpation might cause a certain hemopoietic compensation of the remainder complexes during early spinning. Exogenous hormones such as $20-{\beta}-hydroxyecdysone$ and juvenile hormone analog had a positive effect on hemocytes proliferation.

Immunotherapeutic Effects of Dendritic Cells Pulsed with a Coden-optimized HPV 16 E6 and E7 Fusion Gene in Vivo and in Vitro

Zhou, Zhi-Xiang,Li, Dan,Guan, Shan-Shan,Zhao, Chen,Li, Ze-Lin,Zeng, Yi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Cervical cancer is the second most common cause of cancer related death of women. Persistent HPV infection, especially with high-risk types such as HPV16 and HPV18, has been identified to be the primary cause of cervical cancer. E6 and E7 are the major oncoproteins of high-risk HPVs, which are expressed exclusively in HPV infected tissues, and thereby represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this work, we used recombinant adenovirus expressing coden-optimized HPV16 E6 and E7 fusion protein (Ad-ofE6E7) to prime dendritic cells (DC-ofE6E7), to investigate the ability of primed DC vaccine in eliciting antitumor immunity in vitro and vivo. Results: Our results indicated that DC-ofE6E7 vaccine co-culturing with splenocytes could strongly induce a tumor-specific cytotoxic T lymphocyte (CTL) response and kill the TC-1 cells effectively in vitro. Moreover, DC-ofE6E7 vaccine induced protective immunity against the challenge of TC-1 cancer cells in vivo. Conclusions: The results suggested that the HPV16 ofE6E7 primed DC vaccine has potential application for cervical cancer immunotherapy.

A Novel Mutant of Human Papillomavirus Type 18 E6E7 Fusion Gene and its Transforming Activity

Zhou, Zhi-Xiang,Zhao, Chen,Li, Qian-Qian,Zeng, Yi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Background: Persistent human papillomavirus (HPV) infection, especially with high-risk types such as HPV16 and HPV18, has been identified as the primary cause of cervical cancer. E6 and E7 are the major onco-proteins of high-risk HPVs, which are consistently expressed in HPV infected tissues but absent in normal tissues and represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this study, the optimized fusion gene HPV18 E6E7 (HPV18 ofE6E7) was constructed according to genetic codon usage for human genes. At the same time, for safety future clinical application, a mutant of HPV18 ofE6E7 fusion gene was generated by site-directed mutagenesis at L52G for the E6 protein and C98G for the E7 protein. Results: HPV18-E6E7 mutant (HPV18 ofmE6E7) constructed in this work not only lost the transformation capability for NIH 3T3 cells and tumorigenicity in BALB/c nude mice, but also maintained very good stability and antigenicity. Conclusion: These results suggest that the mutant should undergo further study for application as a safe antigenspecific therapeutic vaccine for HPV18-associated tumors.

Hemocyte Changes after the Extirpation of the Hemopoietic Organ-wing Disc Complexes in the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

( Qing Xiang Zhou ),( Xing Jia Shen ),( Yong Zhu Yi ),( Ai Hua Xia ),( Zhi Fang Zhang ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.13 No.2

We successfully extirpated all four hemopoietic organ-wing disc complexes of the fifth instar larvae of Bombyx mori, and found that most of the treated silkworms could still develop into the moths. We investigated the changes of the circulating hemocytes and evaluated the effects of extirpation on the hemopoiesis. The results showed that proliferation of circulating hemocytes was sufficient to allow development of the silkworms which complexes were totally extirpated. We also found that hemopoitic organ-wing disc complexes extirpation might cause a certain hemopoietic compensation of the remainder complexes during early spinning. Exogenous hormones such as 20-β-hydrox-yecdysone and juvenile hormone analog had a positive effect on hemocytes proliferation.

Hepatic Sinusoidal Obstruction Syndrome Caused by Herbal Medicine: CT and MRI Features

Hua Zhou,Yi-Xiang J. Wang,Hai-yan Lou,Xiao-jun Xu,Min-ming Zhang 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.2

Objective: To describe the CT and MRI features of hepatic sinusoidal obstruction syndrome (HSOS) caused by herbal medicine Gynura segetum. Materials and Methods: The CT and MRI features of 16 consecutive Gynura segetum induced HSOS cases (12 men, 4 women) were analyzed. Eight patients had CT; three patients had MRI, and the remaining five patients had both CT and MRI examinations. Based on their clinical presentations and outcomes, the patients were classified into three categories:mild, moderate, and severe. The severity of the disease was also evaluated radiologically based on the abnormal hepatic patchy enhancement in post-contrast CT or MRI images. Results: Ascites, patchy liver enhancement, and main right hepatic vein narrowing or occlusion were present in all 16 cases. Hepatomegaly and gallbladder wall thickening were present in 14 cases (87.5%, 14/16). Periportal high intensity on T2-weighted images was present in 6 cases (75%, 6/8). Normal liver parenchymal enhancement surrounding the mainhepatic vein forming a clover-like sign was observed in 4 cases (25%, 4/16). The extent of patchy liver enhancement was statistically associated with clinical severity classification (kappa = 0.565). Conclusion: Ascites, patchy liver enhancement, and the main hepatic veins narrowing were the most frequent signs of herbal medicine induced HSOS. The grade of abnormal patchy liver enhancement was associated with the clinical severity.

Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Wu, Xiang-Mei,Liu, Xing,Jiao, Qing-Fang,Fu, Shao-Yue,Bu, You-Quan,Song, Fang-Zhou,Yi, Fa-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

Risk Factors for Cervical Cancer in Rural Areas of Wuhan China: a Matched Case-control Study

Zhang, Bin,Zhou, Ai-Fen,Zhu, Chang-Cai,Zhang, Ling,Xiang, Bing,Chen, Zhong,Hu, Rong-Hua,Zhang, Ya-Qi,Qiu, Lin,Zhang, Yi-Ming,Xiong, Chao-Du,Du, Yu-Kai,Shi, Yu-Qin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Cervical cancer is a serious public health problem in developing countries. We investigated possible risk factors for cervical cancer in rural areas of Wuhan China using a matched case-control study with 33 women diagnosed with cervical cancer and 132 healthy women selected from the same area as matched controls. A questionnaire, which included questions about general demography conditions, environmental and genetic factors, the first sexual intercourse, first marriage age, age at first pregnancy, pregnancy first child's age, female personal health history, social psychological factors, dietary habits, smoking and alcohol status and other living habits was presented to all participants. At the same time, HPV infection of every participant was examined in laboratory testing. Results showed HPV infection (P<0.000, OR=23.4) and pregnancy first child's age (P<0.000, OR=13.1) to be risk factors for cervical cancer. Menopause (P=0.003, OR=0.073) was a protective factor against cervical cancer. However, there was no indication of associations of environmental (drinking water, insecticide, disinfectant) genetic (cancer family history), or life-style factors (smoking status, alcohol status, physical training, sleep quality), including dietary habits (intake of fruit and vegetable, meat, fried food, bean products and pickled food) or social psychological factors with cervical cancer. The results suggest that the risk of cervical cancer in Chinese rural women may be associated with HPV infection, menopause and the pregnancy first child's age.

Down-regulation of the cyclin E1 oncogene expression by microRNA-16-1 induces cell cycle arrest in human cancer cells

( Fu Wang ),( Xiang Dong Fu ),( Yu Zhou ),( Yi Zhang ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.11

Shaking table tests on seismic response of backdrop metal ceilings

Tie G. Zhou,Shuai S. Wei,Xiang Zhao,Le W. Ma,Yi M. Yuan,Zheng Luo 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.32 No.6

In recent earthquakes, the failure of ceiling systems has been one of the most widely reported damage and the major cause of functionality interruption in some buildings. In an effort to mitigate this damage, some scholars have studied a series of ceiling systems including plaster ceilings and mineral wool ceilings. But few studies have involved the backdrop metal ceiling used in some important constructions with higher rigidity and frequency such as the main control area of nuclear power plants. Therefore, in order to evaluate its seismic performance, a full-scale backdrop metal ceiling system, including steel runners and metal panels, was designed, fabricated and installed in a steel frame in this study. And the backdrop metal ceiling system with two perimeter attachments variants was tested: (i) the ends of the runners were connected with the angle steel to form an effective lateral constraint around the backdrop metal ceiling, (ii) the perimeter attachments of the main runner were retained, but the perimeter attachments of the cross runner were removed. In the experiments, different damage of the backdrop metal ceiling system was observed in detail under various earthquakes. Results showed that the backdrop metal ceiling had good integrity and excellent seismic performance. And the perimeter attachments of the cross runner had an adverse effect on the seismic performance of the backdrop metal ceiling under earthquakes. Meanwhile, a series of seismic construction measures and several suggestions that need to be paid attention were proposed in the text so that the backdrop metal ceiling can be better applied in the main control area of nuclear power plants and other important engineering projects.

Chemotherapy and Late Course Three Dimensional Conformal Radiotherapy for Treatment of Patients with Stage III Non-small Cell Lung Cancer

Liu, Yang-Chen,Zhou, Shao-Bing,Gao, Fei,Ye, Hong-Xun,Zhao, Ying,Yi, Xiao-Xiang,Huang, Xin-En,Xiang, Jin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Objective: To compare the efficacy and complications of chemotherapy and late course three-dimensional conformal radiotherapy (3DCRT) in treating patients with stage III non-small cell lung cancer (NSCLC). Patients and Methods: All patients were divided into two groups: to receive chemotherapy and late course 3DCRT (3DCRT group), or chemotherapy and conventional fraction radiation (control group). In the 3DCRT-group, patients were given 6~15 MV X-rays with a total dose of 40 Gy, followed by 3DCRT, 2.5 Gy~3.0 Gy per fraction, 1 fraction/every day, total 68 Gy~70 Gy; in the control group, with conventional fraction radiation the total dose was 64~66 Gy. The chemotherapy regimen in both cases was EP (VP-16 and DDP). Results: Sixty four patients with stage III NSCLC were divided into two groups: 32 patients into 3DCRT, 32 into the control group. One and 2-year survival rates in 3DCRT and control group were 87.5%, 56.3%mad 65.6%, 34.4%, respectively (P<0.05); local control rates were 90.6%, 81.3% and 65.6%, 53.1%, respectively (P<0.05). Conclusion: Chemotherapy and late course 3DCRT is associated with improved survival rate in patients with stage III NSCLC with good tolerability.