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Circulating microRNAs as biomarkers in bile-derived exosomes of cholangiocarcinoma
Jin-Yi Han,Keun Soo Ahn,Yong Hoon Kim,Tae-Seok Kim,Won-Ki Baek,Seong-Il Suh,Koo Jeong Kang 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.101 No.3
Purpose: In this pilot study, using next-generation sequencing and integrated messenger RNA (mRNA) sequencing, we investigated circulating microRNA (miRNA) expression profiling from bile-derived exosomes to identify dysregulated miRNA signatures and oncogenic pathways and determine their effects on targeted mRNAs in cholangiocarcinoma (CCA). Moreover, we explored the possibility that genetic analysis using bile-derived exosomes may replace gene analysis using tissue. Methods: Bile was collected from a patient with perihilar CCA before curative resection. As a control, bile was collected from a patient with a common bile duct stone. Exosomes were isolated from the bile, and we performed next-generation miRNA sequencing using isolated exosomes. To evaluate miRNA-mRNA interactions, mRNA sequencing was performed using bile fluid in both patients. Results: We identified 22 differentially expressed miRNAs. More than 65% of the predicted mRNA targets of those miRNAs were actually differentially expressed between control and CCA bile samples. In functional pathway analysis, targets of 22 miRNAs were primarily enriched in mitogen-activated protein kinase, platelet derived growth factor, vascular endothelial growth factor, epidermal growth factor receptor, and p53 signaling. In particular, in the functional assessment of miRNAmRNA interactions, RAS pathways, including downstream pathways (PI3K-AKT-mTOR and RAS-RAF-MEK-ERK), were determined to be enriched. Conclusion: Circulating miRNAs in bile-derived exosomes provide new information for the development of miRNA analysis in CCA. These miRNAs may represent the oncogenic characteristics of CCA tissue, enabling them to be used instead of tissue samples for the diagnosis of CCA. Further research investigating circulating miRNAs in bile exosomes may lead to more rational, targeted approaches to treatment.
Yi, Seungjun,Kim, Jin-Hyoung,Cho, Yang-Jin,Lee, Jiwon,Choi, Tae-Sup,Cho, Dae Won,Pac, Chyongjin,Han, Won-Sik,Son, Ho-Jin,Kang, Sang Ook American Chemical Society 2016 Inorganic Chemistry Vol.55 No.7
<P>Improvement of the stability of blue phosphorescent dopant material is one of the key factors for real application of organic light-emitting diodes (OLEDs). In this study, we found that the intramolecular hydrogen bonding in an ancillary ligand from a heteroleptic Ir(III) complex can play an important role in the stability of blue phosphorescence. To rationalize the role of intramolecular hydrogen bonding, a series of Ir(III) complexes is designed and prepared: Ir(dfppy)(2)(pic-OH) (1a), Ir(dfppy)(2)-(pic-OMe) (1b), Ir(ppy)(2)(pic-OH) (2a), and Ir(ppy)(2)(pic-OMe) (2b). The emission lifetime of Ir(dfppy)(2)(pic-OH) (1a) (tau(em) = 3.19 mu s) in dichloromethane solution was found to be significantly longer than that of Ir(dfppy)(2)(pic-OMe) (1b) (tau(em) = 0.94 mu s), because of a substantial difference in the nonradiative decay rate (k(nr) = 0.28 x 10(5) s(-1) for (1a) vs 2.99 X 10(5) s(-1) for (1b)). These results were attributed to the intramolecular OH center dot center dot center dot O=C hydrogen bond of the 3-hydroxy-picolinato ligand. Finally, device lifetime was significantly improved when 1a was used as the dopant compared to FIrpic, a well-known blue dopant. Device III (1a as dopant) achieved an operational lifetime of 34.3 h for an initial luminance of 400 nits compared to that of device IV (FIrpic as dopant), a value of 20.1 h, indicating that the intramolecular hydrogen bond in ancillary ligand is playing an important role in device stability.</P>
( Won Jeong Hwang ),( Su Jin Hwang ),( Kyoungsuk Lee ),( Yi Jung Chung ) 물리치료재활과학회 2013 Physical therapy rehabilitation science Vol.2 No.2
Objective: Clinical measures that quantify upper extremity function are needed for the accurate evaluation of patients and to plan an intervention strategy. The purpose of this study was to examine the relationship between the Unified Parkinson`s Disease Rating Scale (UPDRS)-Motor Exam and upper extremity performance as a quantifying clinical tool of upper extremity function in persons with Parkinson`s disease. Design: Cross-sectional study. Methods: Thirty-two idiopathic Parkinson`s Disease persons participated in this study. To investigate the relationship between the UPDRS-motor exam, Box and Block test (BBT), and Action Research Arm Test (ARAT) by two physical therapists. The examination took up to 1 hour, and the participants were invited to rest between each clinical measure in order to minimize the effects of fatigue. Clinical measures were assessed while the subjects were in the “on” phase of their medication cycle, generally 1-3 hour after taking their anti-Parkinson`s medications. Results: In more affected side, the UPDRS-motor exam was significantly negative correlated with the BBT (p<0.05) but it was not significantly correlated with the ARAT. In less affected side, only positively correlation was significantly shown between BBT and ARAT (p<0.05). On the other hand, between BBT and ARAT were not significantly correlated with the UPDRS-motor exam. Conclusions: The UPDRS-motor exam is effective tool which was significantly correlated with manual dexterity in more affected upper extremity. But The UPDRS-motor exam is not effective tool in less affected upper extremity.
Enzyme-Linked Immunosorbent Assay to Detect the MHV Infection in Mice
Yi-Rang Na,Seung-Hyeok Seok,Min-Won Baek,Hui-Young Lee,Dong-Jae Kim,Kyoung-Jin Noh,Sung-Hoon Park,Hyun-Kyoung Lee,Noton Kumar Dutta,Byoung-Hee Lee,Jae-Hak Park 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.4
Mouse hepatitis virus (MHV) is one of the major troublesome infectious diseases in laboratory mice. ELISA techniques generally have been shown to be more sensitive than other diagnostic methods for detection of MHV infection. Here, we developed an ELISA test method by using MHV type-2 strain and it gave reliable test results about detection of MHV infection in mice with high accuracy and low costs.
Yi, Hyo-Seung,Park, Won-Hwan,Lim, Sun-Hee,Moon, Jin-Young The Physiological Society of Korean Medicine and T 2008 동의생리병리학회지 Vol.22 No.5
This study was undertaken to elucidate the antioxidant activity of the ethanol (EEPB) and water (WEPB) extracts of Prunus persica branches. The extracts contained a high phenolic content and revealed a potent hydrogen donating activity in DPPH scavenging assay. Compared to $\alpha$-tocopherol, EEPB (p < 0.001) and WEPB (p < 0.05) significantly inhibited $FeCl_2$-ascorbic acid-induced lipid peroxidation, and also exhibited potent antiradical activities against hydroxyl radical, superoxide anion, nitric oxide and peroxynitrite. In copper- and AAPH-mediated human low-density lipoprotein (LDL) oxidation systems, the extracts demonstrated a strong antioxidant function by metal chelating, rather than direct scavenging, action. Furthermore, EEPB at 5 ${\mu}g/mL$ concentration showed 80.77% inhibition of the electrophoretic mobility of LDL, compared to 77.69% for ascorbic acid and 76.92% for BHT. These results suggest that PB branch extracts may protect against oxidative stress-induced diseases.
Identification of Enzymatic Catalysis of PncA using <sup>1</sup>H-NMR
Yi, Jong-Jae,Kim, Won-Je,Rhee, Jin-Kyu,Lim, Jongsoo,Lee, Bong-Jin,Son, Woo Sung Korean Magnetic Resonance Society 2017 Journal of the Korean Magnetic Resonance Society Vol.21 No.3
Pyrazinamidase (PncA) from Mycobacterium tuberculosis is the hydrolytic enzyme (hydrolase) that can hydrolyze substrate PZA to active form pyrazoic acid (POA). To investigate hydrolytic reaction of M. tuberculosis PncA, 1D NMR spectra were monitored at various molar ratios of PncA and PZA. The line-width of PZA was changed as PncA was added into PZA with different molar ratios. These results suggested that determination of PncA enzymatic activity could potentially serve as an indirect measure of PZA susceptibility.
Characterization of pH-dependent structural properties of hydrolase PncA using NMR
Yi, Jong-Jae,Kim, Won-Je,Rhee, Jin-Kyu,Lim, Jongsoo,Lee, Bong-Jin,Son, Woo Sung Korean Magnetic Resonance Society 2018 Journal of the Korean Magnetic Resonance Society Vol.22 No.4
Catalytic enzyme Pyrazinamidase (PncA) from Mycobacterium tuberculosis can hydrolyze substrate pyrazinamide (PZA) to pyrazoic acid (POA) as active form of compound. Using NMR spectroscopy, pH-dependent catalytic properties were monitored including metal binding mode during converting PZA to POA. There seems to be a conformational change through zinc binding in active site from the perturbation of peak intensities in series of 2D HSQC spectra the conformation changes through zinc binding.
Jin, Cho-Yi,Han, Song-Yi,Kwon, Kisang,Yun, Eun Young,Kang, Seok Woo,Goo, Tae Won,Kim, Seung-Whan,Yu, Kweon,Kwon, O-Yu Walter de Gruyter GmbH 2010 Zeitschrift fur Naturforschung. Section C Vol.65 No.1
<P>Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human granulocyte macrophage colony-stimulating factor (hGM-CSF), which gets its name from the Bm5-hGM-CSF cell in which the glycoprotein of the hGM-CSF is secreted in the cell culture supernatant (CCS). It was demonstrated that secreted hGM-CSF had in vivo biological activity and the white blood cell (WBC) value increased two times that of the control. We expect to produce useful human recombinant glycoproteins from silkworm cultured cells for a low price and a large quantity</P>
Yi, Eun-Sang,Lee, Soo-Hyun,Son, Meong-Hi,Kim, Ju-Youn,Cho, Eun-Joo,Lim, Su-Jin,Cheuh, Hee-Won,Yoo, Keon-Hee,Sung, Ki-Woong,Koo, Hong-Hoe The Korean Pediatric Society 2012 Clinical and Experimental Pediatrics (CEP) Vol.55 No.3
Purpose: This study compared outcomes in children with acute leukemia who underwent transplantations with umbilical cord blood (UCB), bone marrow, or peripheral blood stem cells from a human leukocyte antigen (HLA)-matched related donor (MRD) or an unrelated donor (URD). Methods: This retrospective study included consecutive acute leukemia patients who underwent their first allogeneic hematopoietic stem cell transplantation (HSCT) at Samsung Medical Center between 2005 and 2010. Patients received stem cells from MRD (n=33), URD (n=46), or UCB (n=41). Results: Neutrophil and platelet recovery were significantly longer after HSCT with UCB than with MRD or URD ($p$ <0.01 for both). In multivariate analysis using the MRD group as a reference, the URD group had a significantly higher risk of grade III to IV acute graft-versus-host disease (GVHD; relative risk [RR], 15.2; 95% confidence interval [CI], 1.2 to 186.2; $p$=0.03) and extensive chronic GVHD (RR, 6.9; 95% CI, 1.9 to 25.2; $p$ <0.01). For all 3 donor types, 5-year event-free survival (EFS) and overall survival were similar. Extensive chronic GVHD was associated with fewer relapses (RR, 0.1; 95% CI, 0.04 to 0.6; $p$ <0.01). Multivariate analysis showed that lower EFS was associated with advanced disease at transplantation (RR, 3.2; 95% CI, 1.3 to 7.8; $p$ <0.01) and total body irradiation (RR, 2.1; 95% CI, 1.0 to 4.3; $p$=0.04). Conclusion: Survival after UCB transplantation was similar to survival after MRD and URD transplantation. For patients lacking an HLA matched donor, the use of UCB is a suitable alternative.