http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
mtDNA D-loop 변이로 확인된 한국재래닭의 다양한 모계기원
조창연(Chang-Yeon Cho),이풍연(Pung-Yeon Lee),고응규(Yeoung-Gyu Ko),김학규(Hak Kyu Kim),박미나(Mi-Na Park),연성흠(Seung-Hum Yeon) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.1
우리나라 재래닭의 기원을 구명하기 위해서 mtDNA Dloop 영역을 분석한 결과, 1,231~1,232개의 염기구성되어 있으며, 35개소에서 변이가 관찰되었다. 변이 부위를 이용하여 Haplotype을 분류한 결과, 21종으로 분류되었으며 이중 9개인 GenBank에 미등록된 것으로 밝혀졌다. Hplotype 다양성으로 추정한 한국 재래닭의 유전적 변이성은 중국의 재래닭과 유사한 것으로 추정되었다. Haplotype에 대한 Network 분석 결과, 재래닭은 5개의 Clade로 분류되었다. 이들 Clade에 대한 각 집단의 분포 현황으로 한국 재래닭은 운남성 및 중국 재래닭이 보인 결과와 유사하나, 일본의 재래닭과는 약간 상이한 것으로 밝혀졌다. 이상의 결과로 우리나라 재래닭은 공통선조가 다른 5개 이상의 모계가 중국을 통하여 유래되었으며, 일본에도 전파된 것이 확인되었다. 한편, 일본은 한반도를 유래하지 않은 닭의 유입이 있었던 것으로 추정된다. In this study, we analyzed the mitochondrial DNA D-loop region of Korean native chicken to clarify their phylogenetic relationships, possible maternal origin and routes of introduction into Korea. A 1231-1232 bp DNA fragment from the mtDNA D-loop region was sequenced in 315 chickens from 11 populations, Thirty-five variable sites that defined 21 haplotyes were observed. In Korean native chicken, diversity accounted for 90% of the variation, little differentiation among the strains. The 21 haplotypes clustered into 5 clades which were A, B, C, D and E. These results indicate that Korean chickens were derived from China with multiple origins.
초위성체 표지로 본 한국 재래닭 집단의 분자유전학적 구성
이풍연(Poongyeon Lee),연성흠(Seong-Heum Yeon),김재환(Jae-Hwan Kim),고응규(Yeoung-Gyu Ko),손준규(Jun-Kyu Son),이희훈(Hee-Hoon Lee),조창연(ChangYeon Cho) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.2
초위성체(MS) 표지를이용하여한국재래닭집단의각각의 분자유전학적 특성을 조사하고, 그 평가를 통해 한국 재래닭에 대한 품종 및 계통 분류의 기초를 마련하고자 본 연구를 수행하였다. 또한, 한국 재래닭 집단 내 및 집단간 유전적 변이성을 확인하고, 그 분류 및 특성 평가를 위한 MS 분석 체계를 마련하여 국내 가축유전자원의 관리에 활용코자 하였다. 국내 관리 기관 및 농가 보유 11개 계통의 한국 재래닭 및 상용계 462 수를 대상으로 19개 MS 표지로 분석한 결과, 한국 재래닭 집단은 상용계부터 분자유전학적으로 별개의 집단으로 구분되며, 특히 한국 재래닭 중 긴꼬리닭 계통은 상용계와 국내 토종닭 어느 집단과도 확연히 분리되는 것을 확인하였다. 한국 재래닭 집단 간의 유전거리는 0.11~0.18로 비교적 낮게 나타났으나, 유전적 균일도는 R 계통을 제외하고 0.86~0.88로 코니쉬 계통을 제외한 상용계의 0.95~0.97보다 비교적 낮았다. 다만, 긴꼬리닭 집단의 유전적 균일도는 0.91~0.97로 높게 나타났다. 본 연구를 통하여 한국 재래닭 집단 간의 유전적 차이 및 동질성, 그리고 집단내 의유전적 균일성을 확인하고, 긴꼬리닭 계통의 위치를 확인하였다. 이러한 결과는 국내 유전자원의 고유성을 인정할 수 있는 과학적인 근거로서, 국가 수준의 가축유전자원 평가, 관리의 기초자료로 활용될 수 있을 것이다. The study was conducted to select and optimize microsatellite (MS) markers for evaluate Korean Native Chicken (KNC) breeds in order to provide standard for the classification and breed definition of the indigenous breeds. The study also aimed to characterize and classify each KNC populations for inventory and management of avian genetic resources. A total of 462 chickens from 11 populations of chicken breeds including eight KNC breeds and three commercial chicken breeds were analyzed with 19 MS markers. KNC breeds, especially Long-Tail Chicken breeds, formed separate cluster from those commercial chicken breeds. Genetic distances between KNC populations (0.11~0.18) were relatively shorter. Genetic uniformity of KNC (except KNCR breed) (0.86~0.88) were higher than that of commercial breeds (except Cornish) (0.95~0.97). On the other hand, genetic uniformity of KNC Long Tail (KNCLT) were relatively higher (0.91~0.97). The result can be used to evaluate and manage animal genetic resources at national scale.
Differentially Expressed Genes in Porcine Parthenogenetic 2-cell and Blastocyst Embryos
Hwa-young Lee,Bong-Ki Kim,Xiang-Chun Cui,Kyu-Chan Hwang,Hak-Jae Chung,Yeoung-Gyu Ko,Jin-Kwan Jung,Won-Kyong Chang,N. H. Kim 한국동물생명공학회(구 한국동물번식학회) 2004 Reproductive & Developmental Biology(Supplement) Vol.28 No.2s
Effect of In Vivo Embryo Production and Embryo Transfer Following Superovulation in Hanwoo
Sang-Rae Cho,Sun-Ho Choi,ChangYong Choe,Jun-Kyu Son,Soo-Ho Choi,Young-joo Kim,Poongyeon Lee,Yeoung-Gyu Ko,Sung-Heum Yeon,Jin-Woo Jung,Sung-Jae Kim,Yeon-Sub Jung,Nan-Hee Bok,Hyun-Jong Kim,Dong-Soo Son 한국동물생명공학회(구 한국동물번식학회) 2010 발생공학 국제심포지엄 및 학술대회 Vol.2010 No.1
Park, Mi-Rung,Hwang, In-Sun,Shim, Joo-Hyun,Moon, Hyo-Jin,Kim, Dong-Hoon,Ko, Yeoung-Kyu,Seong, Hwan-Hoo,Im, Gi-Sun The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.3
This study was conducted to investigate the development and gene expression in miniature pig nuclear transfer (mNT) embryos produced under different osmolarity culture conditions. Control group of mNT embryos was cultured in PZM-3 for 6 days. Treatment group of mNT embryos was cultured in modified PZM-3 with NaCl (mPZM-3, 320 mOsmol) for 2 days, and then cultured in PZM-3 (270 mOsmol) for 4 days. Blastocyst formation rate of the treatment group was significantly higher than the control and the apoptosis rate was significantly lower in treatment group. Bax-$\alpha$ and caspase-3 mRNA expression were significantly higher in the control than the treatment group. Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts compared to in vivo derived blastocyst H19 and Xist mRNA expression were significantly lower in the control than the treatment group or in vivo. IGF2 mRNA expression was significantly higher in the control than the treatment group or in vivo. IGF2r mRNA expression was significantly lower in the control. Methylation profiles of individual DNA strands in H19 upstream T-DMR sequences showed a similar methylation status between treatment group and in vivo. These results indicate that the modification of osmolarity in culture medium at early culture stage could provide more beneficial culture environments for mNT embryos.
Mi-Rung Park,In-Sun Hwang,Joo-Hyun Shim,Hyo-Jin Moon,Dong-Hoon Kim,Yeoung-Kyu Ko,Hwan-Hoo Seong,Gi-Sun Im 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.3
This study was conducted to investigate the development and gene expression in miniature pig nuclear transfer (mNT) embryos produced under different osmolarity culture conditions. Control group of mNT embryos was cultured in PZM-3 for 6 days. Treatment group of mNT embryos was cultured in modified PZM-3 with NaCl (mPZM-3, 320 mOsmol) for 2 days, and then cultured in PZM-3 (270 mOsmol) for 4 days. Blastocyst formation rate of the treatment group was significantly higher than the control and the apoptosis rate was significantly lower in treatment group. Bax- and caspase-3 mRNA expression were significantly higher in the control than the treatment group. Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts compared to in vivo derived blastocyst H19 and Xist mRNA expression were significantly lower in the control than the treatment group or in vivo. IGF2 mRNA expression was significantly higher in the control than the treatment group or in vivo. IGF2r mRNA expression was significantly lower in the control. Methylation profiles of individual DNA strands in H19 upstream T-DMR sequences showed a similar methylation status between treatment group and in vivo. These results indicate that the modification of osmolarity in culture medium at early culture stage could provide more beneficial culture environments for mNT embryos.
Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer
Kim, Hyun,Bok, Nan-Hee,Kim, Sung-Woo,Do, Yoon-Jung,Seong, Hwan-Hoo,Kim, Jae-Hwan,Kim, Dong Hun,Kim, Min-Kyu,Ko, Yeoung-Gyu 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.3
Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts (10.2x1014/mol s-1 versus 6.4x1014/mol s-1, p<0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over 12.0x1014/mol s-1, respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.
Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer
Kim, Hyun,Bok, Nan-Hee,Kim, Sung-Woo,Do, Yoon-Jung,Seong, Hwan-Hoo,Kim, Jae-Hwan,Kim, Dong Hun,Kim, Min-Kyu,Ko, Yeoung-Gyu The Korean Society of Embryo Transfer 2014 한국동물생명공학회지 Vol.29 No.3
Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts ($10.2{\times}10^{14}/mol\;s^{-1}$ versus $6.4{\times}10^{14}/mol\;s^{-1}$, p<0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over $12.0{\times}10^{14}/mol\;s^{-1}$, respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.