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허정식,윤여민,홍정연 제주대학교 생명과학연구소 2003 제주생명과학연구 Vol.6 No.1
Purpose: Escherichia coli is the most frequent pathogenic agent of urinary tract infection, causing a wide spectrum of clinical syndromes from asymptomatic cystitis to pyelonephritis or sepsis. We studied the changes of antibiotic sensitivity to E.coli of urinary tract infection for alternative years in order to give some useful informations about the choice of adequate drug in the treatment of UTI. causing E.coli. Materials and Methods: We retrospectively reviewed the hospital charts of 185 cases with causative E.coli of urinary tract infection, who were admitted to or visited the hospital and had more than 10^(5)cfu/ml on urine culture in 1998, 2000, 2001 and 2002. Results: In E.coli isolates, resistance to six antibiotics changed significantly: ampicillin(57.14%, 84.38%, 85.07%, 55.10%), amoxacillin+clavulate(44.44%, 16,42%, 4.17%), ticacillin(84.62%, 75%, 86.67%, 51.14%), ticacillin-clavulate (0%, 51.35%, 11.94%, 4.08%), and trimethoprim(0%, 75%, 59.62%, 28.57%). Conclusion: Using ampicillin, trimethoprim/sulfamethoxazole as the first choice in the treatment of UTI should be reconsidered in Jeju.
Lee, Hye Yeong,Lee, Hye-Lan,Yun, Yeomin,Kim, Jin-Su,Ha, Yoon,Yoon, Do Heum,Lee, Soo-Hong,Shin, Dong Ah Mary Ann Liebert 2015 Tissue engineering. Part A Vol.21 No.13
<P>Stem cells are a promising source of tissue engineering due to their differentiation potential. Today, direct transplantation of stem cells for cell therapy is commonly performed. However, in cases of nerve injury, direct transplantation of cells could lead to secondary nerve damage. Male Sprague-Dawley rats were randomized into four groups: the phosphate-buffered saline epineural transplantation (PBS-ENT) group, the PBS intraneural transplantation (PBS-INT) group, the human adipose-derived stem cells epineural transplantation (hASCs-ENT) group, and human adipose-derived stem cells intraneural transplantation (hASCs-INT) group. Transplantation was conducted 1 week later after inflicting a crush injury with subsequent observation for 5 weeks. To evaluate pain, each group was examined with regard to paw withdrawal latency and evoked potentials. The sciatic functional index (SFI) was calculated to estimate functional recovery. The sciatic nerve was also examined histologically. The hASCs-ENT group showed a more rapid paw withdrawal threshold and SFI recovery than the other groups (p<0.05). The hASCs-ENT group also showed shorter initial latencies in both somatosensory evoked potential (SSEP) and motor evoked potential (MEP) than the PBS-INT group (p<0.05). In addition, the N1 latency of the MEP and the N1 and P1 latencies of the SSEP were significantly shorter than those of the PBS-INT group (p<0.05). Histological examination revealed that the transplanted groups showed better neural recovery and remyelination than the groups injected with PBS. These results show that the transplantation of hASCs into the injured sciatic nerve improved mechanical allodynia and functional recovery as determined by the paw withdrawal test, SFI analysis, and electrophysiological studies. ENT is superior to INT in terms of invasiveness and better outcomes.</P>
Vascular endothelial growth factor-expressing neural stem cell for the treatment of neuropathic pain
Lee, Hye-Lan,Oh, Jinsoo,Yun, Yeomin,Lee, Hye Yeong,You, Youngsang,Che, Lihua,Lee, Minhyung,Kim, Keung Nyun,Ha, Yoon Wolters Kluwer Health | Lippincott Williams Wilkin 2015 NEUROREPORT - Vol.26 No.7
Previously, we determined that vascular endothelial growth factor (VEGF) improves the survival of neural stem cells (NSCs) transplanted into an ischemic environment and effectively enhances angiogenesis. Here, we applied NSCs expressing VEGF (SV-VEGF-NSCs) to treat neuropathic pain. In this study, our goal was to verify the therapeutic effect of SV-VEGF-NSCs by transplanting the cells in a sciatic nerve injury model. We compared the amount of VEGF secreted from DsRed-NSCs (control) or SV-VEGF-NSCs and observed that SV-VEGF-NSCs have a much higher expression level of VEGF. We next investigated whether transplantation with SV-VEGF-NSCs aids functional recovery and pain reduction. We confirmed that transplantation with SV-VEGF-NSCs enhances functional recovery, pain reduction, and remyelination as well as the number of blood vessels compared with the control groups. Our results show that VEGF aids functional recovery and pain reduction in a sciatic nerve injury model.
You, Youngsang,Che, Lihua,Lee, Hye Yeong,Lee, Hye-Lan,Yun, Yeomin,Lee, Minhyung,Oh, Jinsoo,Ha, Yoon Wolters Kluwer Health, Inc. All rights reserved. 2015 1528-1159) Vol.40 No.24
STUDY DESIGN.: Neuronal cell-specific gene expression system and neural stem cells (NSCs) were combined for treatment of spinal cord injury (SCI). OBJECTIVE.: To verify the reproducibility of the neuronal cell-specific therapeutic gene overexpression system, we develop a neuronal cell-specific granulocyte-macrophage colony-stimulating factor expression system (NSE-GMCSF), and then examine the characteristics of GMCSF overexpression and protective effect on neural cells in vitro and vivo. SUMMARY OF BACKGROUND DATA.: The stem cell transplantation is considered a promising therapy for SCI. However, stem cell monotherapy strategy is insufficient for complete recovery after SCI. Therefore, combined treatment method based on stem cells with other therapeutic system may be effective for improving the therapeutic efficacy. In this study, we established the gene and stem cell therapy platform based on NSCs and neuronal cell-specific gene expression system. METHODS.: To examine the GMCSF expression pattern, we compared the amount of secreted GMCSF from the neuronal cell-specific GMCSF expressing NSCs with control GMCSF-expressing NSCs (respectively, NSE-GMCSF-NSCs vs. SV-GMCSF-NSCs) by ELISA in vitro and in vivo, and then verified the neuronal protective effect of these cells in vitro and vivo. RESULTS.: The results showed that NSE-GMCSF-NSCs secreted more GMCSF compared with SV-GMCSF-NSCs in normoxia, hypoxia and cytotoxic conditions. The cell viability of NSE-GMCSF-NSCs was increased depending on the amount of secreted GMCSF in cytotoxic condition. In addition, the amount of secreted GMCSF by NSE-GMCSF-NSCs transplanted into injured spinal cord was significantly higher than SV-GMCSF-NSCs. Higher amount of secreted GMCSF decreased the expression of proapoptotic protein, Bax. CONCLUSION.: In this study, we demonstrated that the neuronal cell-specific gene expression system induced overexpression of GMCSF in NSCs. These combined NSCs & gene therapy treatment protocol would be an effective therapeutic system for SCI.Level of Evidence: N/A