http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Zhangjun Shen,Yupeng Wang,Yansong Chen,Zhen Zhang 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.1
Copper mine tailings are often contaminated by a range of heavy metals, which can restrict plant growth. The aim of this study was to investigate the degree of soil contamination by copper (Cu), zinc (Zn), lead (Pb), cadmium(Cd), chromium (Cr), and manganese (Mn) in a deserted copper mine tailings site, as well as the translocation of theseheavy metals into the rhizosphere soil and the root, fibril and shoot tissues of a tolerant plant, Celosia argentea. Inorder of concentration, the heavy metal concentrations in the soils were Cu ≥ Mn > Zn ≥ Cr > Pb > Cd, and themean concentrations of Cu, Zn, Pb, and Cd in the rhizosphere were 1.8, 1.6, 1.5, and 1.2 times greaterthanin thenon-rhizosphere soil, respectively. The concentrations of all examined heavy metals except for Pb were higher thanthe second-level criterion of the Chinese soil environmental quality standard (CSES) (GB15618-1995) in both soilsamples. The pollution load indexes of Cu, Zn, Pb, Cd, and Cr in the rhizosphere were 28.13, 1.69, 0.148, 8.67, and1.49, respectively, using the CSES. Cu and Cr were mainly stored in the roots of C. argentea, whileCd, Mn, andZn accumulated in the shoots. The translocation factors of Zn, Cd, and Mn were greater than 1, and the Cdenrichment factor of the C. argentea shoots was close to 1. This indicates that C. argentea is a hyperaccumulator ofCd, and could be used in the phytoremediation of Cd-contaminated soils.
Zhou Pinghui,Wei Bangguo,Guan Jingjing,Chen Yu,Zhu Yansong,Ye Yuchen,Meng Yue,Guan Jianzhong,Mao Yingji 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.1
BACKGROUND: Degeneration of the annulus fibrosus (AF), an important structure of the intervertebral disc, is one of the main causes of degenerative disc disease. Fabrication of scaffolds replicating the stratified microstructure of the AF is critical for the successful regeneration of AF. METHODS: In this study, we cultured rabbit AF-derived stem cells (AFSCs) using fabricated electrospun fibrous poly-L-lactic acid scaffolds with different diameters. We applied cyclic tensile strain (CTS) on the scaffolds to regulate the differentiation of AFSCs into specific cell types that resided at the inner, middle, and outer zones of the AF. RESULTS: We found that the morphologies of AFSCs on the smaller-fiber-diameter scaffolds were nearly round, whereas spindle-like cells morphologies were observed on large-diameter scaffolds. CTS enhanced these phenomena and made the cells slender. The expression levels of collagen-I in cells increased as a function of the fiber diameter, whereas collagen-II and aggrecan exhibited opposite trends. Moreover, the application of CTS upregulated the gene expressions of collagen-I, collagen-II, and aggrecan. CONCLUSION: Overlaying the scaffolds with different CTS-stimulated cells could eventually lead to engineered AF tissues with hierarchical structures that approximated the native AF tissue. Thus, the proposed methodologies could be potentially applied for AF regeneration. BACKGROUND: Degeneration of the annulus fibrosus (AF), an important structure of the intervertebral disc, is one of the main causes of degenerative disc disease. Fabrication of scaffolds replicating the stratified microstructure of the AF is critical for the successful regeneration of AF. METHODS: In this study, we cultured rabbit AF-derived stem cells (AFSCs) using fabricated electrospun fibrous poly-L-lactic acid scaffolds with different diameters. We applied cyclic tensile strain (CTS) on the scaffolds to regulate the differentiation of AFSCs into specific cell types that resided at the inner, middle, and outer zones of the AF. RESULTS: We found that the morphologies of AFSCs on the smaller-fiber-diameter scaffolds were nearly round, whereas spindle-like cells morphologies were observed on large-diameter scaffolds. CTS enhanced these phenomena and made the cells slender. The expression levels of collagen-I in cells increased as a function of the fiber diameter, whereas collagen-II and aggrecan exhibited opposite trends. Moreover, the application of CTS upregulated the gene expressions of collagen-I, collagen-II, and aggrecan. CONCLUSION: Overlaying the scaffolds with different CTS-stimulated cells could eventually lead to engineered AF tissues with hierarchical structures that approximated the native AF tissue. Thus, the proposed methodologies could be potentially applied for AF regeneration.
Two New Triterpenoid Acids from Kadsura coccinea
Yan Song,Qing-Jie Zhao,Yong-Sheng Jin,Chuan-Wei Feng,Hai-Sheng Chen 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.12
An investigation of EtOAc extracts of Kadsura coccinea (Lem.) A. C. Smith, has led to the isolation of two new compounds characterized as 3-hydroxy-12-hydroxyl coccinic acid (1) and 3-hydroxy-neokadsuranic acid A (2). Their structures were established by 1D and 2D NMR techniques and mass spectroscopy. Antiproliferative effects of the isolated compounds were evaluated against four human tumor cell lines (A549, HCT116, HL-60 and HepG2), and it was found that compound 1 exhibited antiproliferative effects with IC50 values ranging from 3.01 to 18.08 μg/mL.