Knockdown of circPUM1 impedes cell growth, metastasis and glycolysis of papillary thyroid cancer via enhancing MAPK1 expression by serving as the sponge of miR-21-5p

Yanqi Li,Jun Qin,Zhaocai He,Guang Cui,Kun Zhang,Buqiang Wu 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background Circular RNAs (circRNAs) are a crucial class of regulatory RNAs in cancer procession, including papillary thyroid cancer (PTC). Circ-Pumilio 1 (circPUM1) is a novel circRNA with the oncogenic function in ovarian cancer and lung cancer. However, the role of circPUM1 in PTC is undiscovered. Objective This study was performed to investigate the biological function and molecular mechanism of circPUM1 in PTC. Methods CircPUM1 and microRNA-21-5p (miR-21-5p) levels were analyzed via quantitative real-time polymerase chain reaction (qRT-PCR). Cellular viability and metastasis were measured using Cell Counting Kit 8 (CCK-8) and transwell migration/invasion assay. Glycolysis was evaluated by glucose uptake and lactate production. Associated proteins were examined applying with western blot. Dual-luciferase reporter assay and RNA pull-down assay were used to analyze the interaction between circPUM1 or mitogen-activated protein kinase 1 (MAPK1) and miR-21-5p. Moreover, the role of circPUM1 in vivo was explored by xenograft tumor experiment. Results Signifcantly, circPUM1 was upregulated in PTC tissue samples and cells. Cell growth, metastasis and glycolytic process of PTC cells were all inhibited after downregulation of circPUM1. Besides, circPUM1 could sponge miR-21-5p and MAPK1 was a target gene of miR-21-5p. Furthermore, we found that the anti-cancer efect of circPUM1 knockdown on PTC was partly ascribed to MAPK1 downregulation by upregulating miR-21-5p. Silencing circPUM1 also impeded tumorigenesis of PTC in vivo via miR-21-5p/MAPK1 axis. Conclusion These fndings suggested that circPUM1 knockdown inhibited MAPK1 expression by targeting miR-21-5p, consequently leading to the repressive efect on PTC progression. CircPUM1 might be a promising target to improve the diagnosis and treatment of PTC.

A Study on the Competitiveness of Korean and Japanese Semiconductor Products in the Chinese Market

Yanqi Li,Je Hong, Lee 한국무역금융보험학회(구 한국무역보험학회) 2021 무역금융보험연구 Vol.22 No.5

본 연구는 세계 전체 반도체 2위(메모리 반도체 1위) 생산국인 한국 반도체가 중국시장에서 점유율과 경쟁력 지수를 검증하기 위해 시장점유율지수(MSI), 수출편향지수(EBI)를 활용하여 분석하였다. 특히 기존의 반도체 강국인 일본반도체와 중국시장에서 시장점유율과 경쟁력을 비교 분석하였다. 본 연구를 통해 한국 반도체 시장의 추이분석을 통해 반도체 시장확대의 지 속성을 유지하기 전략을 수립하는데 연구의 목적이 있다. 본 연구는 한국과 일본의 반도체산업에 대상으로 중국시장의 경쟁관계를 분석하였다. 시장점 유율(MSI) 분석결과, 단순히 수치로 살펴보면 10개 대상항목 중에 상대적으로 비교하여 한국 과 일본 각각 반도체 5 부품이 차지했다. 한국은 기억장치(0.88%~11.59%), 부분품(4.71%~ 23.23%), 프로세서와 컨트롤러(2.77%~8.16%), 메모리(16.46%~32.45%), 그 밖의 기기(17.51%~ 41.09%) 5항목의 중국시장점유율이 일본보다 높다. 일본은 그 이외 5항목의 점유율이 한국보 다 높다. 실제로 한국은 중국시장 점유율 10% 이상 품목이 5개 더 많이 있다. 반면에 일본은 한국보다 10% 이상인 반도체 부품이 2개 더 많다. 전체적으로 시장점유율 분석한 결과, 한국 반도체산업은 일본보다 중국시장의 점유율이 높게 나타나고, 중국이 한국과 일본 양국으로부 터 수입된 반도체는 주로 반도체 제조장비와 집적회로이다. 그 중에 평판디스플레이 제조용 기계와 기기는 한국과 일본 양국의 중국시장 점유율이 모두 높아 이를 합치면 중국시장 69.61%~81.39% 차지하고 있다. Purpose : This study analyzed the market share index (MSI) and the export bias index (EBI) to verify the market share and competitiveness index of Korean semiconductors, the world's second largest semiconductor (memory semiconductor) producer, in the Chinese market. In particular, the market share and competitiveness of the existing semiconductor powerhouse, Japanese semiconductor, and the Chinese market were compared and analyzed. The purpose of this study is to establish a strategy to maintain the continuity of semiconductor market expansion through trend analysis of the Korean semiconductor market. Research design, data, methodology : This study analyzed the competitive relationship in the Chinese market for the semiconductor industries of Korea and Japan. As a result of the market share (MSI) analysis, if we simply look at the numbers, 5 semiconductor components in Korea and Japan each accounted for relatively compared among the 10 target items. Results : In Korea, memory devices (0.88%~11.59%), parts (4.71%~23.23%), processors and controllers (2.77%~8.16%), memory (16.46%~32.45%), and other devices (17.51%~41.09%) ) China's market share for item 5 is higher than that of Japan. Conclusions : In Japan, the market share of the other five items is higher than that of Korea. In fact, Korea has five more items with a market share of 10% or more in China. On the other hand, Japan has two more semiconductor parts with 10% or more than Korea. As a result of the overall market share analysis, the Korean semiconductor industry has a higher share of the Chinese market than Japan, and the semiconductors China imported from both Korea and Japan are mainly semiconductor manufacturing equipment and integrated circuits. Among them, flat-panel display manufacturing machines and devices have a high market share in both Korea and Japan, which together account for 69.61% to 81.39% of the Chinese market.

Safety and antifatigue effect of Korean Red Ginseng: a randomized, double-blind, and placebo-controlled clinical trial

Li Zhang,Xiaoyun Chen,Yanqi Cheng,Qilong Chen,Hongsheng Tan,Dongwook Son,Dongpill Chang,Zhaoxiang Bian,Hong Fang,Hongxi Xu 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: Korean Red Ginseng (KRG) is widely used for strengthening the immune system andfighting fatigue, especially in people with deficiency syndrome. However, there is concern that the longtermapplication or a high dose of KRG can cause “fireness” (上火in Chinese) because of its “dryness” (燥性in Chinese). The aim of this study was to assess the safety and efficacy of a 4-week treatment with KRGin participants with deficiency syndrome. Methods: This was a 4-week, randomized, double-blind, placebo-controlled clinical trial. A total of 180Chinese participants were randomly allocated to three groups: placebo control group, participants weregiven a placebo, 3.6 g/d; KRG 1.8 g and 3.6 g groups. The primary outcomes were the changes in firenessand safety evaluation (adverse events, laboratory tests, and electrocardiogram). The secondary outcomeswere the efficacy of KRG on fatigue, which include the following: traditional Chinese medicine (TCM)symptom scale and fatigue self-assessment scale. Results: Of the 180 patients, 174 completed the full study. After 4 weeks of KRG treatment, the Fire-heatsymptoms score including Excess fire-heat score and Deficient fire-heat score showed no significantchange as compared with placebo treatment, and no clinically significant changes in any safetyparameter were observed. Based on the TCM syndrome score and fatigue self-assessment score, TCMsymptoms and fatigue were greatly improved after treatment with KRG, which showed a dose- and timedependenteffect. The total effective rate was also significantly increased in the KRG groups. Conclusion: Our study revealed that KRG has a potent antifatigue effect without significant adverse effectsin people with deficiency syndrome. Although a larger sample size and longer treatment may berequired for a more definite conclusion, this clinical trial is the first to disprove the common conceptionof “fireness” related to KRG.

Safety and antifatigue effect of Korean Red Ginseng: a randomized, double-blind, and placebo-controlled clinical trial

Zhang, Li,Chen, Xiaoyun,Cheng, Yanqi,Chen, Qilong,Tan, Hongsheng,Son, Dongwook,Chang, Dongpill,Bian, Zhaoxiang,Fang, Hong,Xu, Hongxi The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4

Background: Korean Red Ginseng (KRG) is widely used for strengthening the immune system and fighting fatigue, especially in people with deficiency syndrome. However, there is concern that the long-term application or a high dose of KRG can cause "fireness" (上火 in Chinese) because of its "dryness" (燥性 in Chinese). The aim of this study was to assess the safety and efficacy of a 4-week treatment with KRG in participants with deficiency syndrome. Methods: This was a 4-week, randomized, double-blind, placebo-controlled clinical trial. A total of 180 Chinese participants were randomly allocated to three groups: placebo control group, participants were given a placebo, 3.6 g/d; KRG 1.8 g and 3.6 g groups. The primary outcomes were the changes in fireness and safety evaluation (adverse events, laboratory tests, and electrocardiogram). The secondary outcomes were the efficacy of KRG on fatigue, which include the following: traditional Chinese medicine (TCM) symptom scale and fatigue self-assessment scale. Results: Of the 180 patients, 174 completed the full study. After 4 weeks of KRG treatment, the Fire-heat symptoms score including Excess fire-heat score and Deficient fire-heat score showed no significant change as compared with placebo treatment, and no clinically significant changes in any safety parameter were observed. Based on the TCM syndrome score and fatigue self-assessment score, TCM symptoms and fatigue were greatly improved after treatment with KRG, which showed a dose- and time-dependent effect. The total effective rate was also significantly increased in the KRG groups. Conclusion: Our study revealed that KRG has a potent antifatigue effect without significant adverse effects in people with deficiency syndrome. Although a larger sample size and longer treatment may be required for a more definite conclusion, this clinical trial is the first to disprove the common conception of "fireness" related to KRG.

The infertile individual analysis based on whole-exome sequencing in chinese multi-ethnic groups

Zhao Qiongzhen,Li Yanqi,Liang Qi,Zhao Jie,Kang Kai,Hou Meiling,Zhang Xin,Du Renqian,Kong Lingyin,Liang Bo,Huang Weidong 한국유전학회 2023 Genes & Genomics Vol.45 No.4

Background Infertility is a common and rapidly growing health issue around the world. The genetic analysis based on the infertile population is crucial for intervention and treatment. Objective To find candidate gene locus led to azoospermia in Chinese multi-ethnic groups and provide theoretical guidance for the diagnosis of genetic diseases to progressively aggravated infertility patients and sterile offspring with ART. Methods The study based on whole-exome sequencing (WES) was presented for genetic characteristic analysis of multi-ethnics and identification of variants related to infertility in Xinjiang area of China. Results The frequency of pathogenic variants showed significant ethnic differences among four main ethnics in Xinjiang. The population structure analysis confirmed that the Hui was close to the Han population, the Kazak was close to the Uygur population, and there are three ancestry components in the four ethnics. In addition, ten candidate variants potentially regulated azoospermia were detected, and KNTC1 (rs7968222: G > T) was chosen to validate the association. Through the analysis in the valid group, the frequency of rs7968222 (G > T) has a significant difference in the azoospermia population (11.76%, 8/68) and normospermia population (4.63%, 35/756) (P < 0.001). Interestingly, the proportion of people with abnormal follicle-stimulating hormone (FSH) level in the group carrying rs7968222 (G > T) was significantly higher than non-carriers (P < 0.05). Therefore, rs7968222 may regulate spermatogenesis through affecting hormone level. Conclusion Our study establishes the genetics analysis of Northwest China and finds a candidate gene locus KNTC1 (rs7968222: G > T), which is one of the genetic susceptibility factors for male azoospermia.

Scheme and application of phase delay spectrum towards spatial stochastic wind fields

Yan, Qi,Peng, Yongbo,Li, Jie Techno-Press 2013 Wind and Structures, An International Journal (WAS Vol.16 No.5

A phase delay spectrum model towards the representation of spatial coherence of stochastic wind fields is proposed. Different from the classical coherence functions used in the spectral representation methods, the model is derived from the comprehensive description of coherence of fluctuating wind speeds and from the thorough analysis of physical accounts of random factors affecting phase delay, building up a consistent mapping between the simulated fluctuating wind speeds and the basic random variables. It thus includes complete probabilistic information of spatial stochastic wind fields. This treatment prompts a ready and succinct scheme for the simulation of fluctuating wind speeds, and provides a new perspective to the accurate assessment of dynamic reliability of wind-induced structures. Numerical investigations and comparative studies indicate that the developed model is of rationality and of applicability which matches well with the measured data at spatial points of wind fields, whereby the phase spectra at defined datum mark and objective point are feasibly obtained using the numerical scheme associated with the starting-time of phase evolution. In conjunction with the stochastic Fourier amplitude spectrum that we developed previously, the time history of fluctuating wind speeds at any spatial points of wind fields can be readily simulated.