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      • KCI등재

        Isolation and Characterization of a Putative Vacuolar Na+/H+ Antiporter Gene from Zoysia japonica L.

        Yanhua Du,Qian Hei,Yaxin Liu,Hui Zhang,Kai Xu,Tao Xia 한국식물학회 2010 Journal of Plant Biology Vol.52 No.4

        A Na+/H+ antiporter gene from Zoysia japonica (ZjNHX1) which is a member of plant NHX-genes family was cloned by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE)technology. The isolated cDNA is 2,421 bp in length and contains one open-reading frame (ORF) which comprises 540amino acid residues and shows higher homology with other plant Na+/H+ antiporters. ZjNHX1 could partially complement the salt-sensitive phenotypes of Δnhx1 and Δena1-4Δnhx1 yeast mutants in the presence of NaCl, KCl, and LiCl. The expression of ZjNHX1 in Z. japonica increased after NaCl treatment and this result accords with that of Na+contents determination under the same treatment. These results implied that ZjNHX1 functions as a vacuolar Na+/H+antiporter and plays an important role in salt tolerance and ion homeostasis in Z. japonica.

      • SCIESCOPUSKCI등재

        Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation

        Du, Ming,Fu, Xiangwei,Zhou, Yanhua,Zhu, Shien Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.11

        This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the cumulus expansion index (CEI) (p<0.05). To determine the underlying mechanism, the expression levels of several key factors that play crucial roles in cumulus expansion including components of extracellular matrix (ECM) (Has2, Ptgs2, Ptx3, and Tnfaip6) and Growth differentiation factor 9 (GDF9) were measured in control and TSA treated samples by real-time PCR. The effect of TSA on ERK phosphorylation (p-ERK1/2) in cumulus cells and GDF9 protein level in fully grown oocytes (FGOs) were detected by Western blotting. The expression levels of the ECM genes were significantly decreased (p<0.05) by TSA treatment while GDF9 expression did not response to TSA (p>0.05). TSA treatment blocked the activation of ERK1/2 (p<0.05) and had no significant effect on GDF9 protein expression (p>0.05). Collectively, these results suggested that TSA treatment altered ECM gene expression and blocked ERK1/2 activation to inhibit cumulus expansion in the mouse.

      • KCI등재

        Heterologous Expression and Purification of Zea mays Transglutaminase in Pichia pastoris

        Hongbo Li,Yanhua Cui,Lanwei Zhang,Huaxi Yi,Xue Han,Yuehua Jiao,Ming Du,Rongbo Fan,Shuang Zhang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5

        Transglutaminases (TGases) are a family ofenzymes that catalyze the cross-linking of proteins and arewidely used in the food industry to improve the texture ofdairy, meat, and bread products. Zea mays transglutaminase(TGZ) is a new type of TGase with a wide potential. TGZwas expressed in the yeast Pichia pastoris under an alcoholoxidase promoter. Maximal expression of recombinantTGZ was achieved by inducing recombinant GS115(pPIC9K-tgz) in BMMY medium using 1.5% methanol for96 h. Secreted TGZ was initially separated using Superdex200 resin and further purified on cation exchange resin. The activity of TGZ following purification was 0.32 U/mgof protein. The polymerization effect of TGZ on caseincatalyzed by recombinant TGZ was slightly lower than theeffect of microbial transglutaminase (MTG). TGZ is a newpotential additive for the food industry.

      • KCI등재

        A QEE-Oriented Fair Power Allocation for Two-tier Heterogeneous Networks

        ( Shiyu Ji ),( Liangrui Tang ),( Yanhua He ),( Shuxian Li ),( Shimo Du ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.5

        In future wireless network, user experience and energy efficiency will play more and more important roles in the communication systems compared to their roles at present. Quality of experience (QoE) and Energy Efficiency (EE) become the widely used metrics. In this paper, we study a combinatorial problem of QoE and EE and investigate a fair power allocation in heterogeneous networks. We first design a new metric, QoE-aware EE (QEE) to reflect the relationship of QoE and energy. Then, the concept of Utopia QEE is introduced, which is defined as the achievable maximum QEE in ideal conditions, for each user. Finally, we transform the power allocation process to an optimization of ratio of QEE and Utopia QEE and use invasive weed optimization (IWO) algorithm to solve the optimization problem. Numerical simulation results indicate that the proposed algorithm can get converged and efficiently improve the system energy efficiency and the QoE for each user.

      • KCI등재

        Impact of electrolyte additives (alkali metal salts) on the capacitive behavior of NiO-based capacitors

        Yong Zhang,Lizhen Wang,Aiqin Zhang,Yanhua Song,Xiaofeng Li,Xingbing Wu,Peipei Du,Lv Yan 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.2

        To improve the specific capacitance and energy density of electrochemical capacitor, nanostructured NiO was prepared by high temperature solid-state method as electrode material. The crystal structure and morphology of as-parepared NiO samples were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Cyclic voltammetry (CV) measurement was applied to investigate the specific capacitance of the NiO electrode. Furthermore,a novel mixed electrolyte consisting of NaOH, KOH, LiOH and Li_2CO_3 was prepared for the NiO capacitor,and the component and concentration of the four different electrolytes was examined by orthogonal test. The results showed that the NiO sample has cubic structure with nano-size particles, and the optimal composition of the electrolyte was: NaOH 2 mol L^(−1), KOH 3 mol L^(−1), LiOH 0.05 mol L^(−1), and Li_2CO_3 0.05 mol L^−1. At a scan rate of 10 mV s^(−1), the fabricated capacitor exhibits excellent electrochemical capacitive performance, while the specific capacitance and the energy density were 239 F g^(−1) and 85 Wh kg^(−1), which was higher than one-component electrolyte.

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