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- 저자 : Yangsheng Li (검색결과 5 건)
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Lingling Feng,Hui Li,Jingmei Jiao,Ding Li,Li Zhou,Jian Wan,Yangsheng Li 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5
Rice cultivar zhonghua11 (Oryza sativa L. ssp. japonica) plants with decreased sedoheptulose-1, 7- bisphosphatase (SBPase) were obtained by transformation with the rice SBPase antisense gene under the control of the maize ubiquitin promoter. The transgenic and wild-type plants were grown at different nitrogen levels (0.1, 1, or 10 mM NH4NO3). Growth rates of the seedlings were measured by the changes in dry weight, and the photosynthetic carbon reduction activities and the potential efficiency of photosystem II were measured by CO2 assimilation and Fv/Fm, respectively. At low N, there are strong effects on growth and photosynthesis when SBPase was reduced by genetic manipulation. Decreased SBPase activity led to a decrease in the amount of starch accumulated in the leaves at all N levels and the decrease was much more prominent in low N than that in high N, but the starch allocation between shoot and root was unaltered. The analysis of chlorophyll fluorescence and SBPase activity indicated that the decrease of growth and photosynthesis at different N levels were not related to the function of PSII but to the activity of SBPase. Western blot analysis showed the content of SBPase in thylakoid membranes was much more than in the stroma fractions in transgenic plants at low N. Results suggested that low N in addition to a 34% decrease in SBPase activity is sufficient to diminish photosynthesis and limit biomass production. Decreased SBPase activity may reduce the N use efficiency of photosynthesis and growth and alter biomass allocation.
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Transcriptome profile of one-month-old lambs' granulosa cells after superstimulation
Wu, Yangsheng,Lin, Jiapeng,Li, Xiaolin,Han, Bing,Wang, Liqin,Liu, Mingjun,Huang, Juncheng Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.1
Objective: Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role. Methods: In this study, we applied RNA sequencing to analyze and compare gene expression between prepubertal and adult superstimulated follicle granulosa cells in sheep. Results: There were more than 300 genes that significantly differed in expression. Among these differently expressed genes, many extracellular matrix genes (EGF containing Fibulin Like Extracellular Matrix Protein 1, pentraxin 3, adrenomedullin, and osteopontin) were significantly down-regulated in the superstimulated follicles. Ingenuity pathway and gene ontology analyses revealed that processes of axonal guidance, cell proliferation and DNA replication were expressed at higher levels in the prepubertal follicles. Epidermal growth factor, T-Box protein 2 and beta-estradiol upstream regulator were predicted to be active in prepubertal follicles. By comparison, tumor protein P53 and let-7 were most active in adult follicles. Conclusion: These results may contribute to a better understanding of the mechanisms governing the development of granulosa cells in the growing follicle in prepubertal sheep.
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Huaiyang Xiong,Jing Yang,Yangsheng Li 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3
It has been known that SUB1A-1 confers submergence tolerance to rice (Oryza sativa L.). However, two indica rice genotypes, FR13A and Goda Heenati both carrying this gene, exhibited differential tolerance to submergence. Using oligonucleotide microarray combined with suppression subtractive hybridization, we identified a different number of submergence-responsive genes in each genotype. Two-way analysis of variance (ANOVA) of microarray data indicated that there was a high degree of congruency between transcriptional profiles of the two genotypes in response to submergence. Only two genes exhibited an opposing regulation pattern between genotypes after submergence and 324 genes were regulated by submergence only in one genotype and unchanged in their expression in the other, suggesting differential submergence responses. These genes were potentail candidates responsible for differential tolerance observed between genotypes. Gene ontology analysis demonstrated that they were significantly enriched in the processes involved in defense response and flavonoid biosynthesis. The current study provides novel clues for further understanding of the mechanism of submergence tolerance.
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Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos
Zhang, Xuemei,Wang, Liqin,Wu, Yangsheng,Li, Wenrong,An, Jing,Zhang, Fuchun,Liu, Mingjun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10
Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.
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Yang Sheng,Xie JiaJun,Pan ZhiJie,Guan HongMei,Tu YueSheng,Ye YuanJian,Huang ShouBin,Fu ShiQiang,Li KangXian,Huang ZhiWei,Li XiaoQi,Shi ZhanJun,Li Le,Zhang Yang 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-
The meniscus is vital for maintaining knee homeostasis and function. Meniscal calcification is one of the earliest radiological indicators of knee osteoarthritis (KOA), and meniscal calcification is associated with alterations in biomechanical properties. Meniscal calcification originates from a biochemical process similar to vascular calcification. Advanced glycation end products (AGEs) and their receptors (RAGEs) reportedly play critical roles in vascular calcification. Herein, we investigated whether targeting AGE-RAGE is a potential treatment for meniscal calcification. In our study, we demonstrated that AGE-RAGE promotes the osteogenesis of meniscal cells and exacerbates meniscal calcification. Mechanistically, AGE-RAGE activates mTOR and simultaneously promotes ATF4 accumulation, thereby facilitating the ATF4-mTOR positive feedback loop that enhances the osteogenic capacity of meniscal cells. In this regard, mTOR inhibits ATF4 degradation by reducing its ubiquitination, while ATF4 activates mTOR by increasing arginine uptake. Our findings substantiate the unique role of AGE-RAGE in the meniscus and reveal the role of the ATF4-mTOR positive feedback loop during the osteogenesis of meniscal cells; these results provide potential therapeutic targets for KOA.
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