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Zhang Yan,Liang Zhuo,Jiang Mulan,Wan Xia,Gon Yangmin,Zhang Yinbo,Huang Fenghong 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
In modern diet of human being, the ω-6/ω-3ratio of dietary fatty acid was shifted much towards ω-6series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Δ12/Δ15 bifunctional FAD which could not only produce linoleic acid and α-linolenic acid use just oleic acid as substrate but also adjust the ω-6/ω-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.
Dual-layer Fuzzy Control Architecture for the CAS Rover Arm
Hongwei Gao,Yangmin Li,Jinguo Liu,Kun Hong,Yang Zhang 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.5
Since the conventional impedance control method for a rover arm is not suitable for unconstructed environment with uncertainties, a fuzzy inference method which improves the impedance model dynamically is introduced to realize high-precision control. The fuzzy PD control algorithm which applies to the joint control of a rover arm is analyzed in this paper. With the two level control algorithms, a novel dual-layer fuzzy control framework is proposed, which can enhance the control performance significantly. In order to verify the validity and reliability of the designed algorithms, the robotic arm of the CAS rover is considered as an experimental platform. Kinematics and dynamics models of robotic arm are derived at first. Moreover, the fuzzy inference mechanism and implementation process of impedance model parameters are illustrated. Extensive simulations and experimental results show that the control accuracy and the force control of the system have been significantly improved with the proposed dual-layer fuzzy control architecture.
Weifeng Gong,Xiaobo Guo,Yangmin Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.10
Purpose: Hepatitis C virus (HCV) poses a risk of chronic liver disease and threatens a significant number of people worldwide. MicroRNAs (miRNAs) are linked to the regulation of hepatocarcinogenesis. Although miR-373 is required for HCV infection, the underlying mechanisms of miR-373 involvement in HCV replication remain elusive. Materials and Methods: Quantitative reverse transcription PCR assays were performed to detect the abundances of miR-373 and HCV RNA either in Huh 7.5 cells or liver biopsy specimens with HCV infection. Luciferase assay was employed to probe the interactionsbetween miR-373 and interferon regulatory factor 5 (IRF5). Western blot was conducted to investigate the effect of miR-373 and IRF5 on HCV replication and activation of type 1 interferon (IFN) response in JFH1-infected Huh 7.5 cells. Results: HCV infection appeared to be caused by increased miR-373 expression. Addition of miR-373 promoted HCV RNA expression,while miR-373 depletion led to an inhibitive effect on HCV replication. Concordantly, IRF5, as a direct target, was limitedby miR-373 in JFH1-infected Huh 7.5 cells. In addition, introduction of IRF5 protected HCV replication in the presence of abundant miR-373. Furthermore, the miR-373-mediated inhibitory effect on type 1 IFN response was ablated following IRF5 accumulation. Conclusion: miR-373 abrogation reduced HCV replication via activation of type 1 IFN responses by targeting IRF5 in JFH1-infectedHuh 7.5 cells, suggesting a promising therapeutic for treating HCV infection.
MAO SHEN,CHENGLIN WU,WENPING JIA,CHENGHONG LI,ZHILI ZHANG,YANGMIN JIN,GUODONG FAN,CAIPING LIN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7
Mesostructured chitosan-coated Fe 3 O 4 nanoparticles (CS-coated Fe 3 O 4 NPs) were synthesizedby a facile one-step solvothermal method via using chitosan as a surface-modi¯cation agent. Subsequently, the surfaces of CS-coated Fe 3 O 4 NPs were successfully conjugated with folic acid(FA) molecules to obtain FA – CS-coated Fe 3 O 4 NPs for improving targeted drug delivery. Themorphology, chemical component and magnetic property of as-prepared composite nanoparticleswere characterized by Fourier transform infrared spectroscopy (FTIR), X-ray di®raction (XRD),dynamic light scattering (DLS), scanning transmission electron microscopy (SEM), transmissionelectron microscopy (TEM), thermal gravimetric analysis (TGA) and vibrating sample magne-tometer (VSM). Furthermore, doxorubicin hydrochloride (DOX) as a model drug was encap-sulated for investigating drug release pattern in vitro. The results show that the magnetizationsaturation value of FA – CS-coated Fe 3 O 4 NPs was about 28.5 emu/g, exhibiting super-paramagnetic properties and mesostructure. DOX could be loaded to FA – CS-coated Fe 3 O 4 NPswith high capacity about 27.9%, and the release rate of DOX could be adjusted by the pH value. This work demonstrates that the prepared magnetic nanoparticles have potential applications inthe treatment of cancer as targeting drug delivery carriers.