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        The Effect of pH on the Efficiency of an SBR Processing Piggery Wastewater

        LiLong Yan,Yu Liu,Yuan Ren,Xiaohui Wang,Haijing Liang,Ying Zhang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6

        To treat piggery wastewater efficiently, thehydrolysis of urea (mainly derived from swine urine) inpiggery wastewater with the change of sewage pH must beconsidered. Using activated sludge, piggery wastewaterwas treated in a sequencing batch reactor (SBR), and theeffects of influent pH on SBR processing efficiency, sludgesettle ability, and sludge activity were investigated. Theresults showed that a high influent pH value contributed tothe improvement of the removal rate of ammonia nitrogenand reduction of the chemical oxygen demand (COD). When the influent pH was between 9.0 and 9.5, the removalrate of ammonia nitrogen was higher than 90%, and thereduction of COD from its original value was 80%. Theinfluent pH had a greater influence on sludge concentrationand sludge activity. When the influent pH increased from7.0 to 9.5, the sludge concentration increased from 2,350 to3,947 mg/L in the reactor, and the activities of ammoniumoxidizing bacteria (AOB) and nitrite oxidizing bacteria(NOB) first increased and then decreased. When theinfluent pH was 9.0 and 8.0, the maximum values (0.48 gO2/(g MLSS/day) and 0.080 g O2/(g MLSS/day)) werereached, and the sludge settling ratio was nearly steadybetween 20 and 35% in each reactor.

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        Thymol Ameliorates Aspergillus fumigatus Keratitis by Downregulating the TLR4/ MyD88/ NF-kB/ IL-1β Signal Expression and Reducing Necroptosis and Pyroptosis

        Wang Limei,Yan Haijing,Chen Xiaomeng,Han Lin,Liu Guibo,Yang Hua,Lu Danli,Liu Wenting,Che Chengye 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.1

        Fungal keratitis is a refractory kind of keratopathy. We attempted to investigate the antiinflammatory role of thymol on Aspergillus fumigatus (A. fumigatus) keratitis. Wound healing and fluorescein staining of the cornea were applied to verify thymol’s safety. Mice models of A. fumigatus keratitis underwent subconjunctival injection of thymol. The anti-inflammatory roles of thymol were verified by hematoxylin-eosin (HE) staining, slit lamp observation, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blotting. In contrast with the DMSO group, more transparent corneas and less inflammatory cells infiltration were detected in mice treated with 50 μg/ml thymol. Thymol downregulated the synthesis of TLR4, MyD88, NF-kB, IL-1β, NLRP3, caspase 1, caspase 8, GSDMD, RIPK3 and MLKL. In summary, we proved that thymol played a protective part in A. fumigatus keratitis by cutting down inflammatory cells aggregation, downregulating the TLR4/ MyD88/ NF-kB/ IL-1β signal expression and reducing necroptosis and pyroptosis.

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