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- 저자 : Xuewei Tao (검색결과 3 건)
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Xu, Tao,Sun, Xuewei,Jiang, Shiling,Ren, Dongtao,Liu, Guoqin Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, $M_{396-734}$ corresponding to amino acids Q396-N734 was fused with 6$\times$His-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of $M_{396-734}$ is not activated by $Ca^{2+}$, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 ${\mu}M$, much higher than that of other kinesins reported. The cosedimentation assay indicated that $M_{396-734}$ could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.
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Dongheng Xu,Han Wang,Xuewei Tao,Zhengjun Yao,Shasha Zhang,Moliar Oleksander 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5
The Inconel 718 (IN718) alloy coatings were successfully fabricated using electron beam wire-feeding deposition technology.The macrostructure, microstructure and elemental analysis of the deposited coatings were characterized by OM, SEMand EDS. Moreover, the hardness and wear resistance were also investigated experimentally. The results showed that thecross section of the deposited coatings can be divided into three different regions: clad zone (CZ), fusion zone (FZ) and heataffected zone. Equiaxed dendrites appeared in the CZ while columnar dendrites occurred in the FZ, and discrete fine Lavesphase particles were formed under low beam current while continuous coarse Laves phase particles were found under highbeam current. The EDS results showed that the degree of Nb segregation in FZ is higher than that in CZ. More importantly,the microstructure coarsened and the degree of Nb segregation increased with the increase of beam current. The depositedcoating under the lowest beam current (10 mA) has the highest hardness (263 HV0.2) and the minimum specific wear rate(3.95391 × 10−15 m3/Nm), which is corresponding to the fine microstructure, discrete Laves phase particles and low degreeof Nb segregation under low beam current.
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3-D Deformation Behavior Simulation of Cable Stitch Based on Particle System in Weft Knitted Fabrics
Sha Sha,Lei Luo,Zhongmin Deng,Dapeng Yuan,Bin Li,Xuewei Jiang,Hui Tao,Qufu Wei 한국섬유공학회 2018 Fibers and polymers Vol.19 No.9
The static simulation of weft knitting can be efficiently realized by graphics simulation techniques, but there still remains a challenge for mechanical models. The lack of practical mechanical models significantly limit the realistic deformation behaviors of complex cable stitches, which lead to a great different between the simulation effect and the actual fabric. In order to obtain the deformation behavior and volumetric performance of cable stitch, loop models were built based on an improved particle system in this work. Compared with plain weft knitted, the offset value of bonding points of cable stitches were measured. By analyzing the relationship between the deformation of loops and the displacement of the particles, the deformation behavior of cable stitch was simulated. Velocity-Verlet integration was introduced to simulate cable stitches and the stable results were obtained. The results show that these models and algorithm displayed the accurate deformation behavior of cable stitches, as demonstrated by qualitative comparisons to measure the deformations of actual samples.
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