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RISS 인기검색어
- 검색키워드
- 저자 : Xudong Ren (검색결과 3 건)
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Genome-wide scan for runs of homozygosity identifies candidate genes in Wannan Black pigs
Wu Xudong,Zhou Ren,Zhang Wei,Cao Bangji,Xia Jingjing,Wang Caiyun,Zhang Xiaodong,ChuMing-Xing,Yin Zongjun,Ding Yueyun 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12
Objective: Runs of homozygosity (ROH) are contiguous lengths of homozygous genotypes that can reveal inbreeding levels, selection pressure, and mating schemes. In this study, ROHs were evaluated in Wannan Black pigs to assess the inbreeding levels and the genome regions with high ROH frequency.Methods: In a previous study, we obtained 501.52 GB of raw data from resequencing (10×) of the genome and identified 21,316,754 single-nucleotide variants in 20 Wannan Black pig samples. We investigated the number, length, and frequency of ROH using resequencing data to characterize the homozygosity in Wannan Black pigs and identified genomic regions with high ROH frequencies.Results: In this work, 1,813 ROHs (837 ROHs in 100 to 500 kb, 449 ROHs in 500 to 1,000 kb, 527 ROHs in >1,000 kb) were identified in all samples, and the average genomic inbreeding coefficient (FROH) in Wannan Black pigs was 0.5234. Sixty-one regions on chromosomes 2, 3, 7, 8, 13, 15, and 16 harbored ROH islands. In total, 105 genes were identified in 42 ROH islands, among which some genes were related to production traits.Conclusion: This is the first study to identify ROH across the genome of Wannan Black pigs, the Chinese native breed of the Anhui province. Overall, Wannan Black pigs have high levels of inbreeding due to the influence of ancient and recent inbreeding due to the genome. These findings are a reliable resource for future studies and contribute to save and use the germplasm resources of Wannan Black pigs. Objective: Runs of homozygosity (ROH) are contiguous lengths of homozygous genotypes that can reveal inbreeding levels, selection pressure, and mating schemes. In this study, ROHs were evaluated in Wannan Black pigs to assess the inbreeding levels and the genome regions with high ROH frequency. Methods: In a previous study, we obtained 501.52 GB of raw data from resequencing (10×) of the genome and identified 21,316,754 single-nucleotide variants in 20 Wannan Black pig samples. We investigated the number, length, and frequency of ROH using resequencing data to characterize the homozygosity in Wannan Black pigs and identified genomic regions with high ROH frequencies. Results: In this work, 1,813 ROHs (837 ROHs in 100 to 500 kb, 449 ROHs in 500 to 1,000 kb, 527 ROHs in >1,000 kb) were identified in all samples, and the average genomic inbreeding coefficient (FROH) in Wannan Black pigs was 0.5234. Sixty-one regions on chromosomes 2, 3, 7, 8, 13, 15, and 16 harbored ROH islands. In total, 105 genes were identified in 42 ROH islands, among which some genes were related to production traits. Conclusion: This is the first study to identify ROH across the genome of Wannan Black pigs, the Chinese native breed of the Anhui province. Overall, Wannan Black pigs have high levels of inbreeding due to the influence of ancient and recent inbreeding due to the genome. These findings are a reliable resource for future studies and contribute to save and use the germplasm resources of Wannan Black pigs.
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Jujie Ren,Miao Zhai,Min Cui,Liujia Li,Congcong Yu,Xudong Yu,Xueping Ji 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.1
A superoxide anion radical (O2 ˙-) nonenzymatic electrochemical sensor based on PtRuCu ternary alloy nanoparticles/electrochemically reduced graphene oxide (ERGO) composite modified glassy carbon electrode (PtRuCu/ERGO/GCE) was developed. ERGO and PtRuCu/ERGO composites were characterized by scanning electron microscopy. The prepared PtRuCu/ERGO/ GCE showed remarkable performance toward the electrocatalytic reduction of (O2 ˙-). The sensor possessed a high sensitivity of 1725µA · mM-1 · cm-2, a wide linear range of 7.5–330 µM (R2 = 0.993) and a low detection limit of 0.7 µM (S/N = 3), as well as excellent selectivity, reproducibility and stability, which can be attributed to the synergistic effect of ERGO and PtRuCu ternary alloy nanoparticles. Therefore, the prepared sensor is promising for electrochemical determination of O2˙-.
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The characteristics investigation under the unsteady cavitation condition in a centrifugal pump
Jiaxing Lu,Shouqi Yuan,Parameswaran Siva,Jian-Ping Yuan,Xudong Ren,Banglun Zhou 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3
Numerical simulation and experimental method are combined to investigate the pump inlet and outlet pressure fluctuations, the vibration characteristics and the internal flow instabilities under the unsteady cavitation condition in a centrifugal pump. It is found that the unsteady cavitation starts to generate as the NPSHa is lower than 5.93 m. Apparent asymmetric and uneven cavity volume distribution on each blade and in the impeller can be observed as the NPSHa decreases from 4.39 m to 1.44 m, which includes the cavitation develops from cavitation surge, rotating cavitation to asymmetric cavitation. The flow vortexes in each blade channel are produced in the cavity trailing edges by the shedding and collapse of cavitation, which interfere with each other and aggravate the flow instabilities. The dominant frequencies of the pump inlet and outlet pressure fluctuations are the shaft frequency and blade passing frequency under the unsteady cavitation conditions, respectively. Broadband pulses are obtained from both the pump inlet and outlet pressure pulsations, which results from the random shedding and collapse of unsteady cavitation bubbles. Obvious corresponding relationship between the root mean squares of the vibration measured in different positions and the suction performance curve is found under both the non-cavitation and unsteady cavitation conditions.
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