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Yuan Linlin,Tian Xiufen,Zhang Yanfei,Huang Xinhui,Li Qing,Li Wencai,Li Shenglei 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Laryngeal squamous cell carcinoma (LSCC) is one of the most common subtypes of head and neck malignancies worldwide. Long intervening/intergenic noncoding RNAs (LINCRNAs) have been recently implicated in various biological processes that take place in the setting of laryngeal cancer, but the regulatory role of LINC00319 in LSCC remains largely unknown. The current study aimed to elucidate the regulatory effect of LINC00319 on the development and progression of LSCC via high-mobility group box 3 (HMGB3). Microarray-based analysis was initially conducted to identify differentially expressed long noncoding RNAs, after which the expression of LINC00319 and HMGB3 in LSCC tissues and cells was determined accordingly. CD133 + CD144 + TU177 cells were subsequently isolated and transfected with LINC00319 overexpression vector (oe-LINC00319), short hairpin RNA (sh)-LINC00319, sh-HMGB3, sh-E2F transcription factor 1 (E2F1), and oe-E2F1, as well as their corresponding controls. The proliferative, invasion, self-renewal, and tumorigenic abilities of CD133 + CD144 + TU177 cells were then evaluated. Our in vitro findings were further confirmed following subcutaneous injection of cells expressing the corresponding plasmids into nude mice. LINC00319 and HMGB3 expressions were elevated in LSCC cells and tissues. LINC00319 increased HMGB3 expression by recruiting E2F1. Furthermore, the stimulatory role of LINC00319 on the proliferation, invasion, self-renewal ability, and tumorigenicity of CD133 + CD144 + TU177 cells was achieved by upregulating HMGB3 via recruitment of E2F1. The in vitro findings were also confirmed by in vivo experiments. Taken together, these data show that downregulating LINC00319 in CD133 + CD144 + TU177 cells may serve as a potential anticancer regimen by inhibiting the proliferation and invasion of cancer stem cells in LSCC.
Remote control system based on the internet and machine vision for tracked vehicles
Shuai Wang,Shubing Zhang,Ruoding Ma,E. Jin,Xinhui Liu,He Tian,Ruipeng Yang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.3
A remote control system based on the Internet and machine vision is designed in this study for tracked vehicles. The system consists of remote monitoring, remote control, and visual navigation subsystems. The on-board computer receives control commands issued by the monitoring center and controls the speed and steering angle of the tracked vehicles. The front camera collects navigation line information, and the collected image is processed by filtering, gray processing, binarization processing, and interference elimination to obtain the center point coordinates of the navigation line. Proportional-integral-derivative (PID), fuzzy PID, and neural network control algorithms are compared based on the distance and angle deviations of the preview point to control the operation of tracked vehicles through simulation. The Internet remote control system with the fuzzy PID control algorithm is then tested on a tracked vehicle. Experimental results indicate that the trajectory of the tracked vehicle fits the navigation path well.
Maoke Liu,Ke Zhao,Yuming Tang,Daoqun Ren,Wanchun Yao,Xinhui Tian,Xinyu Zhang,Bin Yi,Bo Deng 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3
Chinese Luzhou-flavor liquor is prepared using distillation of mixed, fermented grains, such as sorghum and rice. Fermentation occurs in cellars lined with pit mud containing microbes. The identity and community diversity of bacteria in pit mud of fermentation cellars contributes to the aromatic compounds of the liquor. In order to determine the contribution of genus Clostridium to this fermentation, the community diversity of Clostridium cluster I in pit mud of different cellar ages (1, 50, 100, and 400 years) was investigated based on denaturing gradient gel electrophoresis followed by 16S rRNA sequencing. Differences in the community structure within pit mud depended on wall and bottom layer cellar locations. A better understanding of the role of Clostridium in Luzhou-flavor liquor manufacture will be useful.