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Bi Hongxia,Sun Xingyuan,Shi Hanbing,Ou Zhimin 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1
Tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate was synthesized using asymmetric reduction of tertbutyl (S)-6-chloro-5-hydroxy-3-oxo-hexanoate with liquid-core immobilized Saccharomyces cerevisiae CGMCC No. 2233. The optimum conditions for preparation of the liquid-core immobilized cells were found to be 2% guar gum,5% CaCl2, 0.8% sodium alginate, capsule diameter 2mm, 0.3% chitosan (1.0×105) solution, and 30 min for formation of the film of liquid-core immobilized cells. The optimum re-cultivation time was 32 h. The optimum reduction conditions were found to be pH 6.8-7.2, 160 r/min, and 30 oC. Conversion was found to reach 100% when initial concentration of substrate was less than 50 g/L. The diastereomeric excess of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate exceeded 99%. The liquid-core immobilized cells retained their effectiveness even after 15 uses.
Jing Yang,Wang Xingyuan,Zhou Tingting,Wei Liangjun,Guo Meiling,Liu Yanan,Sun Xiaoqi,Wang Yanjie 한국탄소학회 2024 Carbon Letters Vol.34 No.3
This paper presents the construction and characterization of an amperometric immunosensor based on the graphene/gold nanoparticles (AuNPs/GO) nanocomposite for the detection of the bladder cancer biomarker, apolipoprotein A1 (Apo-A1). The morphological analysis of the AuNPs/GO nanocomposite demonstrated an almost spherical shape of AuNPs and the successful coverage of their surface by graphene oxide. An increased G peak and decreased D peak after the association of AuNPs with GO, implied a reduction in graphene defects. The X-ray photoelectron spectroscopy (XPS) indicated a significant decrease in the quantity of oxygen-containing functional groups in the AuNPs/GO nanocomposite, as compared to the original GO. Furthermore, the developed sensor demonstrated commendable sensitivity and selectivity, with a wide linear range for Apo-A1 detection. Importantly, the immunosensor exhibited remarkable stability over a period of 14 days, signifying its potential for practical applications.
Yuan Lu,Hongqian Dai,Pengpeng Cheng,Hanbing Shi,Lan Tang,Xingyuan Sun,Zhimin Ou 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.5
A modular approach was applied for the synthesis of bienzyme-polymer nanoconjugates (nano-BECs) (50- 70 nm) consisting of two enzymes (carbonyl reductase and glucose dehydrogenase) conjugated within a single universal polymer scaffold. The amount of the product ethyl (R)-2-hydroxy-4-phenylbutyrate (R-HPBE) with nano-BECs as the catalyst was 533mM in a dibutyl phthalate-phosphate buffer (dibutyl phthalate-PB) biphasic system, while the amount of R-HPBE was 349mM using carbonyl reductase-poly(acrylic acid) as the catalyst, indicating that the nano- BECs have an advantage for coenzyme regeneration. Compared with a single aqueous phase, the substrate treatment capacity was improved at the interface of the dibutyl phthalate-PB biphasic system. Under the optimal reaction conditions (35 oC, 40 h, dibutyl phthalate-PB 1 : 1), nano-BECs can completely convert substrate into optically pure R-HPBE (enantiomeric excess (e.e.) >99.9%) in the organic-aqueous system.
Zhimin Ou,Xiaoyan Chen,Guoqing Ying,Hanbing Shi,Xingyuan Sun 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2
In this study, (S)-3-hydroxy-3-phenylpropionate was prepared continuously by coupling microbial transformation and membrane separation. The effect of several factors on membrane flux, reactor capacity, and reaction conversion were investigated. A kinetic model of the continuous reduction process was also developed. The appropriate molecular weight cut-off of the ultrafiltration membrane was 30 kDa. The reactor capacity reached a maximum of 0.136/h at a biomass concentration and membrane flux of 86 g/L (dry weight/reaction volume) and 20 mL/h, respectively. The (S)-3-hydroxy-3-phenylpropionate yield was 3.68 mmol/L/day after continuous reduction over seven days. The enantiometric excess of (S)-3-hydroxy-3-phenylpropionate reached above 99.5%. The kinetic constants of continuous reduction were as follows:r_m = 3.00 × 10^(−3) mol/L/h, k_(cat) = 3.49 × 10^(−4) mol/L/h, k_1 =3.09 × 10^(−2) mol/L, and k_2 = 5.00 × 10^(−7) mol/L. The kinetic model was in good agreement with the experimental data obtained during continuous reduction. Compared with batch reduction, continuous reduction can significantly improve the catalytic efficiency of microbial cells and increase the reactor capacity.
Lyu Zhaoyun,Liu Haigang,Zhou Ting,Feng Min,Lu Zengbin,Nie Lei,Wang Guiping,Wang Guangzhao,Li Lili,Men Xingyuan,Sun Meng,Yu Yi 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1
The solitary mason bee Osmia excavata Alfken (Hymenoptera: Megachilidae) is an important pollinator of fruit trees in northern China. This stduy compared and studied the flight ability of O. excavata at different temper atures both during and post diapause, with the aim of exploring the feasibility of using O. excavata prematurely released from diapause for pollination. The flight capacity of male and female O. excavata during diapause and post diapause was measured at 3 ◦ C intervals from 10 to 31 ◦ C by using a computer-monitored flight-mill system. The results showed that as the temperature increased, the flight ability also increased until an optimal tem perature was reached, whereupon it gradually decreased regardless of the further increase in temperature. Temperature had a highly significant impact on flight ability (P < 0.01), whereas gender only had a significant effect on average flight distance (P = 0.034). Diapause status had significant effects on average flight distance, average flight speed and maximum flight speed, and the interaction between diapause and temperature had a highly significant impact on average flight distance and average flight speed (P < 0.01). Post diapause, the flying ability of bees was significantly improved; however, diapausing O. excavata demonstrated a sufficient flying ability and so could be used as auxiliary pollinators for fruits in greenhouses during winter and early spring, when there is a shortage of pollinators.