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A Ghost-Imaging System Based on a Microfluidic Chip
Kaimin Wang,Xiaoxuan Han,Hualong Ye,Zhaorui Wang,Leihong Zhang,Jiafeng Hu,Meiyong Xu,Xiangjun Xin,Dawei Zhang 한국광학회 2021 Current Optics and Photonics Vol.5 No.2
Microfluidic chip technology is a research focus in biology, chemistry, and medicine, for example. However, microfluidic chips are rarely applied in imaging, especially in ghost imaging. Thus in this work we propose a ghost-imaging system, in which we deploy a novel microfluidic chip modulator (MCM) constructed of double-layer zigzag micro pipelines. While in traditional situations a spatial light modulator (SLM) and supporting computers are required, we can get rid of active modulation devices and computers with this proposed scheme. The corresponding simulation analysis verifies good feasibility of the scheme, which can ensure the quality of data transmission and achieve convenient, fast ghost imaging passively.
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Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration
Shi Yong,Zhang Xiaoxuan,Liu Rui,Shao Xiaoyan,Zhao Yuanjin,Gu Zhuxiao,Jiang Qing 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Volumetric muscle loss (VML) due to various reasons may cause motor dysfunction and tissue engineering has been proposed for muscle regeneration. However, developing three-dimensional (3D) tissue-engineered scaffolds that can mimic oriented cell growth of muscle tissues are challenging for regeneration medicine. Herein, we propose a novel self-curling 3D oriented scaffold (SCOS) composed of fish derived gelatin methacrylate (GelMA) and fish scales for repairing skeletal muscles.Fish scales of tilapia were decellularized and decalcified. Then, SCOSs were constructed by ultraviolet-coating methylated fish gelatin on the back of fish scales. C2C12 myoblasts were cultured on SCOSs, and after induction of myogenic differentiation, SCOS/C2C12 transplants were prepared for in vivo experiments.Decellularized and decalcified fish scales (DDFSs) became soft and retained the original oriented microgroove surface structure that could induce oriented cell growth. SCOSs could self-curl into 3D structures when immersing in culture medium due to different swelling properties of fish GelMA and DDFSs. Cell experiments demonstrated that SCOSs enhanced the oriented growth and myogenic differentiation of C2C12 myoblasts. By integrating SCOSs and myogenic differentiated C2C12 myoblasts, the resultant SCOS/C2C12 transplants promoted de novo muscle regeneration and functional restoration of muscle activity in the mouse model of VML.Our results suggest that SCOSs loaded with myogenic differentiated C2C12 myoblasts can promote muscle regeneration in mice with skeletal muscle injuries, indicating application prospects of such scaffolds in muscle tissue engineering and other related fields.
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Mingxing Luo,Jiru Zhang,Xiaoxuan Liu,Cai Wu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8
Studies show that the shear strength of angular-shaped calcareous sand is mainly affected by dilatancy, interparticle locking, and particle breakage. In the present study, a series of consolidated-drained triaxial compression tests on calcareous sands are carried out to investigate the strength impacts of dilatancy, interparticle locking, and particle breakage. It is found that as the stress level increases, the friction angle difference reflecting the effect of the particle breakage increases. In contrast, the friction angle difference reflecting the interparticle locking and dilatancy effect decreases gradually. Moreover, the critical stress ratio of the high-angled calcareous sands decreases, which may be attributed to particle breakage. The obtained results demonstrate that when the stress level exceeds a certain value, the critical stress ratio remains constant. Then the correlation between the particle breakage, interparticle locking, and dilatancy effects in the shear strength is studied. Finally, based on the performed analysis on energy dissipation in calcareous sands, a dilatancy model is proposed that covers the particle breakage effect. The performed analyses demonstrate that the proposed model can accurately explain the experimental data.
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Construction of Customer Appeal Classification Model Based on Speech Recognition
Sheng Cao,Yaling Zhang,Shengping Yan,Xiaoxuan Qi,Yuling Li 한국정보처리학회 2023 Journal of information processing systems Vol.19 No.2
Aiming at the problems of poor customer satisfaction and poor accuracy of customer classification, this paperproposes a customer classification model based on speech recognition. First, this paper analyzes the temporaldata characteristics of customer demand data, identifies the influencing factors of customer demand behavior,and determines the process of feature extraction of customer voice signals. Then, the emotional associationrules of customer demands are designed, and the classification model of customer demands is constructedthrough cluster analysis. Next, the Euclidean distance method is used to preprocess customer behavior data. The fuzzy clustering characteristics of customer demands are obtained by the fuzzy clustering method. Finally,on the basis of naive Bayesian algorithm, a customer demand classification model based on speech recognitionis completed. Experimental results show that the proposed method improves the accuracy of the customerdemand classification to more than 80%, and improves customer satisfaction to more than 90%. It solves theproblems of poor customer satisfaction and low customer classification accuracy of the existing classificationmethods, which have practical application value.
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Echinacoside Prevents Sepsis-Induced Myocardial Damage via Targeting SOD2
Jingxiang Wu,Xingji You,Xin Li,Zuojing Zhang,Xiaoxuan Zhang,Yibo Yin,Xinru Yuan 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.2
Echinacoside (ECH) is a prominent naturally occurring bioactive compound with effects of alleviatingmyocardial damage. We aimed to explore the beneficial effects of ECH against sepsis-induced myocardial damage andelucidate the potential mechanism. Echocardiography and Masson staining demonstrated that ECH alleviates cardiac functionand fibrosis in the cecal ligation and puncture (CLP) model. Transcriptome profiling and network pharmacology analysisshowed that there are 51 overlapping targets between sepsis-induced myocardial damage and ECH. Subsequently, chemicalcarcinogenesis-reactive oxygen species (ROS) were enriched in multiple targets. Wherein, SOD2 may be the potential targetof ECH on sepsis-induced myocardial damage. Polymerase chain reaction results showed that ECH administration couldmarkedly increase the expression of SOD2 and reduce the release of ROS. Combined with injecting the inhibitor of SOD2, thebeneficial effect of ECH on mortality, cardiac function, and fibrosis was eliminated, and release of ROS was increased afterinhibiting SOD2. ECH significantly alleviated myocardial damage in septic mice, and the therapeutic mechanism of ECH isachieved by upregulating SOD2 which decreased the release of ROS.
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