Relation between Ki-67, ER, PR, Her2/neu, p21, EGFR, and TOP II-α Expression in Invasive Ductal Breast Cancer Patients and Correlations with Prognosis

Yan, Jian,Liu, Xiao-Long,Han, Lu-Zhe,Xiao, Gang,Li, Ning-Lei,Deng, Yi-Nan,Yin, Liang-Chun,Ling, Li-Juan,Yu, Xiao-Yuan,Tan, Can-Liang,Huang, Xiao-Ping,Liu, Li-Xin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

The aim of the present study was to investigate the expression of the transcription factor Ki-67, ER, PR, Her2/neu, p21, EGFR, and TOP II-${\alpha}$ in the tumor tissue of patients with invasive ductal carcinoma(IDC); in addition, we examined correlations between these markers. Two hundred and sixteen IDC patients, who were not previously been treated with chemo- or radiotherapy, were included in the study. All tumors were grade I-III. Expression of molecular markers was determined by immunohistochemical analysis on paraffin-embedded tissue sections. Follow-up data were collected for 3 months to 10 years and analyzed for tumor recurrence, survival time, and prognostic risk factors. We determined Ki-67 expression correlates with the expression of ER, PR, HER-2, EGFR, and TOP-${\alpha}$, as well as lymph node involvement, high tumor grade, lymphovascular invasion, high tumor stage, and high TNM stage in IDC. Positive Ki-67 expression was a risk factor for rapid tumor recurrence and may help tumor progression, leading to poor prognosis in IDC. Ki-67 was directly correlated with EGFR, TOP II-${\alpha}$, lymph node involvement, high tumor grade, lymphovascular invasion, high tumor stage, and high TNM stage in the hormone receptor subtypes of breast cancer. In triple negative breast cancer, Ki-67 correlated with TOP II-${\alpha}$. Expression of Ki-67 correlated with that of ER, PR, HER-2, EGFR, TOP II-${\alpha}$, and p21. In addition, the biomarker Ki-67 has a role as a prognostic factor and indicates a poor prognosis in IDC.

Value of contrast-enhanced ultrasonography in microwave ablation treatment of symptomatic focal uterine adenomyosis

Xiao-Long Li,Jia-Xin Li,Song-Yuan Yu,Pei-Li Fan,Yun-Jie Jin,Er-Jiao Xu,Sai-Nan Guan,Er-Ya Deng,Qiu-Yan Li,Zheng-Biao Ji,Jiu-Ling Qi,Hui-Xiong Xu,China Alliance of Multi-Center Clinical Study for Ultra 대한초음파의학회 2024 ULTRASONOGRAPHY Vol.43 No.1

Purpose: This study evaluated the value of contrast-enhanced ultrasonography (CEUS) in the ultrasound-guided microwave ablation (MWA) treatment of symptomatic focal uterine adenomyosis.Methods: This retrospective study was conducted between March 2020 and January 2023, enrolling 52 patients with symptomatic focal uterine adenomyosis who had undergone MWA. All patients were examined with CEUS before and after MWA. The non-perfused volume (NPV) was compared between CEUS and dynamic contrast-enhanced magnetic resonance imaging (DCEMRI) following ablation. Therapeutic efficacy and safety were evaluated at 3-, 6-, and 12-month follow-ups. Additionally, this study explored the correlations between pre-treatment CEUS features and a volume reduction ratio indicating sufficient ablation, defined as 50% or more at the 3-month follow-up.Results: No significant differences in NPV were noted between CEUS and DCE-MRI immediately after MWA and during follow-up (all P>0.05). At the 3-month follow-up, the median VRRs for the uterus and adenomyosis were 33.2% and 63.9%, respectively. Sufficient ablation was achieved in 69.2% (36/52) of adenomyosis cases, while partial ablation was observed in the remaining 30.8% (16/52). The identification of non-enhancing areas on pre-treatment CEUS was associated with sufficient ablation (P=0.016). At the 12-month follow-up, significant decreases were observed in both the uterine and adenomyosis volumes (all P<0.001). Dysmenorrhea and menorrhagia were significantly alleviated at 12 months, and no major complications were encountered.Conclusion: CEUS can be used to evaluate the ablation zone of focal adenomyosis that has been treated with MWA, similarly to DCE-MRI. The identification of non-enhancing areas on pretreatment CEUS indicates satisfactory treatment outcomes. Purpose: This study evaluated the value of contrast-enhanced ultrasonography (CEUS) in the ultrasound-guided microwave ablation (MWA) treatment of symptomatic focal uterine adenomyosis. Methods: This retrospective study was conducted between March 2020 and January 2023, enrolling 52 patients with symptomatic focal uterine adenomyosis who had undergone MWA. All patients were examined with CEUS before and after MWA. The non-perfused volume (NPV) was compared between CEUS and dynamic contrast-enhanced magnetic resonance imaging (DCE- MRI) following ablation. Therapeutic efficacy and safety were evaluated at 3-, 6-, and 12-month follow-ups. Additionally, this study explored the correlations between pre-treatment CEUS features and a volume reduction ratio indicating sufficient ablation, defined as 50% or more at the 3-month follow-up. Results: No significant differences in NPV were noted between CEUS and DCE-MRI immediately after MWA and during follow-up (all P>0.05). At the 3-month follow-up, the median VRRs for the uterus and adenomyosis were 33.2% and 63.9%, respectively. Sufficient ablation was achieved in 69.2% (36/52) of adenomyosis cases, while partial ablation was observed in the remaining 30.8% (16/52). The identification of non-enhancing areas on pre-treatment CEUS was associated with sufficient ablation (P=0.016). At the 12-month follow-up, significant decreases were observed in both the uterine and adenomyosis volumes (all P<0.001). Dysmenorrhea and menorrhagia were significantly alleviated at 12 months, and no major complications were encountered. Conclusion: CEUS can be used to evaluate the ablation zone of focal adenomyosis that has been treated with MWA, similarly to DCE-MRI. The identification of non-enhancing areas on pre- treatment CEUS indicates satisfactory treatment outcomes.

Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis

Xiao-wei Zhao,Yong-xin Yang,Dong-wei Huang,Guang-long Cheng,Hui-ling Zhao 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milkyield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitistreatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changesof mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total of 95differentially expressed proteins were identified. Of these, 56 proteins were categorized according to molecular function, cellular component,and biological processes. The most frequent biological processes influenced by the proteins were response to stress, transport, andestablishment of localization. Furthermore, a network analysis of the proteins with altered expression in mammary tissues demonstrated thatthese factors are predominantly involved with binding and structural molecule activities. Vimentin and -enolase were central “functionalhubs” in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands andpotential markers for the effective treatment of E. coli mastitis were identified. These data have also helped elucidate defense mechanismsthat protect the mammary glands and promote the pathogenesis of E. coli mastitis.

Evaluation of insecticidal activity of the essential oil of Allium chinense G. Don and its major constituents against Liposcelis bostrychophila Badonnel

Xin Chao Liu,Xiao Nan Lu,Qi Zhi Liu,Zhi Long Liu 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

Water-distilled essential oil from the dried bulbs of Allium chinense (Liliaceae) was analyzed by gaschromatography–mass spectrometry (GC–MS). Eighteen compounds, accounting for 98.4% of the total oil,were identified and the main components of the essential oil of A. chinense were methyl allyl trisulfide(30.7%), dimethyl trisulfide (24.1%), methyl propyl disulfide (12.8%) and dimethyl disulfide (9.6%) followed bymethyl allyl disulfide (3.4%) and methyl propyl trisulfide (3.6%). The essential oil exhibited contact toxicityagainst the booklice (Liposcelis bostrychophila) with an LC50 value of 441.8 μg/cm2 while the two major constituents,dimethyl trisulfide and methyl propyl disulfide had LC50 values of 153.0 μg/cm2 and 738.0 μg/cm2 againstthe booklice, respectively. The essential oil of A. chinense possessed strong fumigant toxicity against the booklicewith an LC50 value of 186.5 μg/l while methyl allyl trisulfide (LC50=90.4 μg/l) and dimethyl trisulfide (LC50=114.2 μg/l) exhibited stronger fumigant toxicity than methyl propyl disulfide (LC50= 243.4 μg/l) and dimethyldisulfide (LC50= 340.8 μg/l) against the booklice. The results indicated that the essential oil and itsmajor constituentshave potential for development into natural insecticides or fumigants for control of insects in stored grains.

A Computer Model for Simulating the Bicycle Rider's Behavior in a Virtual Riding System

( Wei-long Ding ),( Xiao Ding ),( Kai Chen ),( Zang-xin Wan ),( Yan Xu ),( Yuan-jing Feng ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.3

People are increasingly demanding to experience realistic behavior of virtual characters in computer games. In this article, we build a computer model for simulating a bicycle rider’s behavior in a virtual riding system. A method to calculate the speed of a bicycle rider is proposed to improve the reality in a virtual riding system. In this method, the property of physical energy is introduced, and the bicycle-riding speed is calculated in real time according to the relationship between the rider’s physical energy and bicycle-riding speed. Then based on the analysis of the behavior of a cyclist in a real competition, various behaviors of the virtual rider are designed and a behavior-tree for the virtual bicycle rider is constructed accordingly. On the basis of these, a virtual riding system is developed. The experiments results show that our system can simulate the behavior of a virtual bicycle rider, and thus encourage exercise on a stationary bicycle.

Transducer analysis and signal processing of PMSF with embedded bluff body

Yan, Xiao-Xue,Xu, Ke-Jun,Xu, Wei,Yu, Xin-Long,Wu, Jian-Ping Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.2

Permanent magnet sodium flowmeter (PMSF) have been used to measure the sodium flow in fast breeder reactors. Due to the effects of irradiation, thermal cycling, time lapse, etc., the magnetic flux density of the PMSF will decrease after being used in the reactor for a period of time. Therefore, it must be calibrated regularly. But some flowmeters that immersed in sodium cannot be removed for an off-line calibration, so the on-line calibration is required. However, the best online calibration accuracy of PMSF using cross-correlation analysis method was 2.0-level without considering the repeatability. In order to further improve this work, the operational principle of the transducer in PMSF is analyzed and the design principle of the transducer is proposed. The transducers were tested on the sodium flow loop to collect the experimental data. The signal characteristics are analyzed from the time and frequency domains, respectively. The cross-correlation analysis method based on biased estimation is adopted to obtain the flow rate. The verification experimental results showed that the measurement accuracy is 1.0-level when the flow velocity is above 0.5 m/s, and the measurement accuracy is 3.0-level when the flow velocity is in the range of 0.2 m/s to 0.5 m/s.

Fatty acid-binding protein 5 activates cyclooxygenase-2 and promotes hypoxic injury in LO2 cells

Pan Long,Pang Yihan,Xiao Heng,Deng Xin,Chen Qingsong 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1

Background Hepatic hypoxia always results in liver inflammation and dysfunction. Fatty acid-binding protein 5 (FABP5) has been reported as a hypoxia-inducible gene and a regulator of inflammatory reaction . Objective To evaluate the effects of FABP5 on hypoxic liver injury and its potential mechanisms. Results FABP5 was hypoxia-inducible expression in liver tissues and LO2 cells. The expression levels of IL6 and TNFα were upregulated with the increasing expression of FABP5 in LO2 cells. Downregulation of FABP5 expression decreased the expression levels of ALT, AST and increased cell viability of hypoxic LO2 cells. Downregulation of FABP5 expression inhibited the cyclooxygenase-2/prostaglandin E2 (COX-2/PGE2) pathway and decreased inflammatory reaction in hypoxic LO2 cells. Conclusions FABP5 was shown to activate COX-2/PGE2 pathway and promote hypoxic injury in LO2 cells. FABP5 may be a potential target for the treatment of hypoxic liver injury.

Leaky-Wave Antennas Based on Noncutoff Substrate Integrated Waveguide Supporting Beam Scanning From Backward to Forward

Yue-Long Lyu,Xiao-Xin Liu,Peng-Yuan Wang,Erni, Daniel,Qun Wu,Cong Wang,Nam-Young Kim,Fan-Yi Meng Institute of Electrical and Electronics Engineers 2016 IEEE transactions on antennas and propagation Vol.64 No.6

<P>In this paper, we propose an approach to realize substrate integrated waveguide (SIW)-based leaky-wave antennas (LWAs) supporting continuous beam scanning from backward to forward above the cutoff frequency. First, through phase delay analysis, it was found that SIWs with straight transverse slots support backward and forward radiation of the -1-order mode with an open-stopband (OSB) in between. Subsequently, by introducing additional longitudinal slots as parallel components, the OSB can be suppressed, leading to continuous beam scanning at least from -40 degrees through broadside to 35 degrees. The proposed method only requires a planar structure and obtains less dispersive beam scanning compared with a composite right/left-handed (CRLH) LWA. Both simulations and measurements verify the intended beam scanning operation while verifying the underlying theory.</P>

The Interactive Modeling Method of Virtual City Scene Based on Building Codes

( Wei-long Ding ),( Xiao-jie Zhu ),( Bin Xu ),( Yan Xu ),( Kai Chen ),( Zang-xin Wan ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.1

For higher-level requirements of urban planning and management and the recent development of "digital earth" and "digital city", it is urgent to establish protocols for the construction of three-dimensional digital city models. However, some problems still exist in the digital technology of the three-dimensional city model, such as insufficient precision of the three-dimensional model, not optimizing the scene and not considering the constraints of building codes. In view of those points, a method to interactively simulate a virtual city scene based on building codes is proposed in this paper. Firstly, some constraint functions are set up to restrict the models to adhere to the building codes, and an improved directional bounding box technique is utilized to solve the problem that geometric objects may intersect in a virtual city scene. The three-dimensional model invocation strategy is designed to convert two-dimensional layouts to a three-dimensional urban scene. A Leap Motion hardware device is used to interactively place the 3D models in a virtual scene. Finally, the design and construction of the three-dimensional scene are completed by using Unity3D. The experiment shows that this method can simulate urban virtual scenes that strictly adhere to building codes in a virtual scene of the city environment, but also provide information and decision-making functions for urban planning and management.

Prevalence and Genotype Distribution of Human Papillomavirus among Women from Henan, China

Wang, Xiao-Chuan,Sun, Liang-Qi,Ma, Li,Li, Hua-Xin,Wang, Xiu-Li,Wang, Xin,Yun, Tian,Meng, Nian-Long,Lv, Da-Le Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Human papillomavirus (HPV) infection has been implicated as a causative of cervical cancer. In the present study, a total of 578 samples from females attending the gynecological outpatient clinic in Henan province, China, were collected and the HPV genotypes were detected by gene chip and flow-through hybridization. Overall, 44.5% (257/578) females were found to be HPV DNA positive, and the high risk HPV (HR-HPV) rate was 35.1% (203/578). The first peak of HR-HPV infection appeared in the >60 year-old group (55.0%), and the second was within the 51-55 year-old group (50.0%) (${\chi}^2$=19.497, p<0.05). HPV 16 was the most prevalent genotype (9.2%), followed by HPV 52 (7.8%), HPV 6 (6.9%), HPV 11 (5.9%) and HPV 42 (5.0%). The single type HPV infection was 30.4%, with the five majority prevalent genotype HPV 16 (16.5%), HPV 52 (14.3%), HPV 6 (12.6%), HPV 42 (8.6%), HPV 31 (5.1%). The multiple-type HPV infections were 14.0%, and HPV 16 was the most prevalent type (29.6%), followed by HPV 52 (24.7%), HPV 6 (22.2%), HPV 11 (22.2%), HPV 42 (17.3%) and HPV 39 (17.3%).