Targeting treatment of bladder cancer using PTK7 aptamer-gemcitabine conjugate

Xiang Wei,Peng Yongbo,Zeng Hongliang,Yu Chunping,Zhang Qun,Liu Biao,Liu Jiahao,Hu Xing,Wei Wensu,Deng Minhua,Wang Ning,Liu Xuewen,Xie Jianfei,Hou Weibin,Tang Jin,Long Zhi,Wang Long,Liu Jianye 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Gemcitabine (GEM) is one of the first-line chemotherapies for bladder cancer (BC), but the GEMs cannot recognize cancer cells and have a low long-term response rate and high recurrence rate with side effects during the treatment of BC. Targeted transport of GEMs to mediate cytotoxicity to tumor and avoid the systemic side effects remains a challenge in the treatment of BC.Based on a firstly confirmed biomarker in BC-protein tyrosine kinase 7 (PTK7), which is overexpressed on the cell membrane surface in BC cells, a novel targeting system protein tyrosine kinase 7 aptamer-Gemcitabine conjugate (PTK7-GEMs) was designed and synthesized using a specific PTK7 aptamer and GEM through auto-synthesis method to deliver GEM against BC. In addition, the antitumor effects and safety evaluation of PTK7-GEMs was assessed with a series of in vitro and in vivo assays.PTK7-GEMs can specifically bind and enter to BC cells dependent on the expression levels of PTK7 and via the macropinocytosis pathway, which induced cytotoxicity after GEM cleavage from PTK7-GEMs respond to the intracellular phosphatase. Moreover, PTK7-GEMs showed stronger anti-tumor efficacy and excellent biosafety in three types of tumor xenograft mice models.These results demonstrated that PTK7-GEMs is a successful targeted aptamer-drug conjugates strategy (APDCs) to treat BC, which will provide new directions for the precision treatment of BC in the field of biomarker-oriented tumor targeted therapy.

Lymph Node Ratio is an Independent Prognostic Factor in Node Positive Rectal Cancer Patients Treated with Preoperative Chemoradiotherapy Followed by Curative Resection

Zeng, Wei-Gen,Zhou, Zhi-Xiang,Wang, Zheng,Liang, Jian-Wei,Hou, Hui-Rong,Zhou, Hai-Tao,Zhang, Xing-Mao,Hu, Jun-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: The lymph node ratio (LNR) has been shown to be an important prognostic factor for colorectal cancer. However, studies focusing on the prognostic impact of LNR in rectal cancer patients who received neoadjuvant chemoradiotherapy (CRT) followed by curative resection have been limited. The aim of this study was to investigate LNR in rectal cancer patients who received neoadjuvant chemoradiotherapy (CRT) followed by curative resection. Materials and Methods: A total of 131 consecutive rectal cancer patients who underwent neoadjuvant CRT and total mesorectal excision were included in this study. Patients were divided into two groups according to the LNR (${\leq}0.2$ [n=86], >0.2 [n=45]) to evaluate the prognostic effect on overall survival (OS) and disease-free survival (DFS). Results: The median number of retrieved and metastatic lymph node (LN) was 14 (range 1-48) and 2 (range 1-10), respectively. The median LNR was 0.154 (range 0.04-1.0). In multivariate analysis, LNR was shown to be an independent prognostic factor for both overall survival (hazard ratio[HR]=3.778; 95% confidence interval [CI] 1.741-8.198; p=0.001) and disease-free survival (HR=3.637; 95%CI 1.838-7.195; p<0.001). Increased LNR was significantly associated with worse OS and DFS in patients with <12 harvested LNs, and as well as in those ${\geq}12$ harvested LNs (p<0.05). In addition, LNR had a prognostic impact on both OS and DFS in patients with N1 staging (p<0.001). Conclusions: LNR is an independent prognostic factor in ypN-positive rectal cancer patients, both in patients with <12 harvested LNs, and as well as in those ${\geq}12$ harvested LNs. LNR provides better prognostic value than pN staging. Therefore, it should be used as an additional prognostic indicator in ypN-positive rectal cancer patients.

Fermentation Optimization and Industrialization of Recombinant Saccharopolyspora erythraea Strains for Improved Erythromycin A Production

Xiang Zou,Chang-fa Chen,Xia-chang Qi,Jiang-chao Qian,Ju Chu,Ying-ping Zhuang,Si-liang Zhang,Wei Zeng,Wan-jun Li 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6

Improvement of Erythromycin A (Er-A) production and purity by metabolic engineering of the industrial erythromycin-producing strains Saccharopolyspora erythraea strians ZL1004 and ZL1007, in which the amounts of tailoring enzymes EryK (a P450 hydroxylase)and EryG (an S-adenosylmethionine-dependent O-methyltransferase)for biotransformation of Erythromycin D to Er-A were modulated, was performed in a 50 L fermentor. Addition of 15 g/L of corn steep liquor to the medium increased Er-A production; maximum Er-A production was 8,196 U/mL at 191 h, which was 81.8% higher than that of control (4,507 U/mL at 184 h). Er-B impurities were completely eliminated, whereas Er-C impurities were only 153 U/mL at 191 h. Analysis of intra- and extracellular metabolites and key enzyme activities in central carbon metabolism revealed that the pool of TCA cycle intermediates was enhanced by the addition of corn steep liquor and induced an increase in erythromycin biosynthesis. There were no significant differences between strains ZL1004 and ZL1007 regarding Er-A production and impurity accumulation. Compared to wild type strain,Er-A production was improved by 23.9% while Er-C was reduced by 83.9% and Er-B was completely eliminated. Furthermore, fermentation of recombinant strain ZL1004was successfully scaled up from laboratory scale (50 L fermentor) to industrial scale (25 and 132 m3), with similar levels of Er-A production and purity obtained.

Experimental and numerical studies of stress/strain characteristics in riveted aircraft lap joints

Chao Zeng,Wei Tian,Xiang Yao Liu,Jiu Tian Xue 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.7

The fatigue property of riveted lap joint is greatly related to the riveting-induced residual stress, especially the stress distribution on the faying surface. However, an accurate study of the residual stress characteristics in the riveted sheet could be very difficult. In this paper, both numerical and experimental investigations were carried out on the stress/strain characteristics in riveted aircraft lap joints. A special specimen was designed for the test of strain variations on the faying surface of the sheet by microstrain gages. For the numerical simulation, the rivet squeezing process was analyzed using the explicit dynamic finite element (FE) method, whilst a general static FE analysis was employed for the elastic springback after the squeeze force was removed. A comparison of the strain variations between the experimental results and FE simulations shows a general good agreement, although there may be some difference for points measured near the hole surface. The FE analysis reveals that both compressive and tensile residual stresses could be introduced in the riveted sheet. Massive compressive residual stress can be created in the near-surface layer of the hole. However, the stress level is not always increased with increasing the squeeze force, and so is the improvement of fatigue life observed. Further study is still necessary to account for the fatigue life decreasing effect caused by a high squeeze force.

A Novel Control Strategy for Input-Parallel-Output-Series Inverter System

Song, Chun-Wei,Zhao, Rong-Xiang,Lin, Wang-Qing,Zeng, Zheng Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.2

This paper presents a topology structure and control method for an input-parallel-output-series(IPOS) inverter system which is suitable for high input current, high output voltage, and high power applications. In order to ensure the normal operation of the IPOS inverter system, the control method should achieve input current sharing(ICS) and output voltage sharing(OVS) among constituent modules. Through the analysis in this paper, ICS is automatically achieved as long as OVS is controlled. The IPOS inverter system is controlled by a three-loop control system which is composed of an outer common-output voltage loop, inner current loops and voltage sharing loops. Simulation results show that this control strategy can achieve low total harmonic distortion(THD) in the system output voltage, fast dynamic response, and good output voltage sharing performance.

Structure and Performances of xLiFePO4/C·(1 − x)Li3V2(PO4)3/C Cathode for Lithium-Ion Batteries by Using Poly(vinyl alcohol) as Carbon Source

Chang-ling Fan,Wei-hua Zhang,Tao-tao Zeng,Ling-fang Li,Xiang Zhang,Shao-chang Han 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.11

Poly(vinyl alcohol), whose pyrolysis carbon possesses high conductivity of 8.88 × 10−1 S/cm, was used to synthesize xLiFePO4/C·(1 − x)Li3V2(PO4)3/C cathode. It was characterized by X-ray diffraction, scanning electron microscopy, conductivity, cyclic voltammetry, and galvanostatic charge and discharge experiments. Results show that LiFePO4/C and Li3V2(PO4)3/C coexists in the cathode. The particles sizes of 0.75LiFePO4/C·0.25Li3V2(PO4)3/C (x = 0.75) are much smaller than 100 nm due to the role of poly(vinyl alcohol). Its conductivity is 8.79 × 10−2 S/cm. The oxidative and reductive peaks in cyclic voltammetry are sharp and symmetrical. Their low potential gaps indicate that the extractions and insertions of lithium ion possess excellent reversibility. Its discharge capacities at 1 and 5 C are 141.1 and 100.1 mAh/g. The more Li3V2(PO4)3/C in cathode results in the deterioration of electrochemical performances due to its low theoretical capacity. It is concluded that poly(vinyl alcohol) is an effective carbon source in the preparation of xLiFePO4/C·(1 − x)Li3V2(PO4)3/C composite cathode with excellent performances.

Learner Perceptions of Demotivators in the EFL Classroom : Conceptual Framework and Scale Development

Jianling Xie,Tianlan Wei,Yan Zhan,Yan Zeng,Xiang Huang,Xia Liu,Katarzyna Gallo 아시아영어교육학회 2021 The Journal of Asia TEFL Vol.18 No.4

Grounded in social cognitive theory and expectancy-value theory, the current study aimed to present a demotivation scale — Learner Perceptions of Demotivators Scale (LPDS) — designed specifically for use in L2 research and tested empirically to provide evidence of its construct, validity, and reliability. Study 1 sample consisted of 295 Chinese college English learners. An exploratory factor analysis offered preliminary support for a factor structure comprising three dimensions: negative teacher behavior, loss of task value, and low expectancy for success. Study 2 sample consisted of another 320 Chinese college English learners. The proposed factor structure was further corroborated through confirmatory factor analysis, and support for its validity was provided by means of correlating the three dimensions with academic performance and self-efficacy measures. Specifically, all the correlations were negative except for the positive association between academic performance and negative teacher behavior. Further, whereas the model fit confirmed a well-fitting second-order model, one low first-order loading (negative teacher behavior) does not seem to support a second-order factor model. Therefore, the three dimensions should be regarded as separate to best capture the nuances of different demotivators. By establishing a nomological network (demotivation, academic performance, and self-efficacy), the current study illuminates selected aspects of ESL pedagogy.

Asparagus Polysaccharide and Gum with Hepatic Artery Embolization Induces Tumor Growth and Inhibits Angiogenesis in an Orthotopic Hepatocellular Carcinoma Model

Weng, Ling-Ling,Xiang, Jian-Feng,Lin, Jin-Bo,Yi, Shang-Hui,Yang, Li-Tao,Li, Yi-Sheng,Zeng, Hao-Tao,Lin, Sheng-Ming,Xin, Dong-Wei,Zhao, Hai-Liang,Qiu, Shu-Qi,Chen, Tao,Zhang, Min-Guang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Liver cancer is one of leading digestive malignancies with high morbidity and mortality. There is an urgent need for the development of novel therapies for this deadly disease. It has been proven that asparagus polysaccharide, one of the most active derivates from the traditional medicine asparagus, possesses notable antitumor properties. However, little is known about the efficacy of asparagus polysaccharide as an adjuvant for liver cancer chemotherapy. Herein, we reported that asparagus polysaccharide and its embolic agent form, asparagus gum, significantly inhibited liver tumor growth with transcatheter arterial chemoembolization (TACE) therapy in an orthotopic hepatocellular carcinoma (HCC) tumor model, while significantly inhibiting angiogenesis and promoting tumor cell apoptosis. Moreover, asparagine gelatinous possessed immunomodulatory functions and showed little toxicity to the host. These results highlight the chemotherapeutic potential of asparagus polysaccharide and warrant a future focus on development as novel chemotherapeutic agent for liver cancer TACE therapy.

Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA

Chunping Yu,Yi Zhang,Ning Wang,Wensu Wei,Ke Cao,Qun Zhang,Peiying Ma,Dan Xie,Pei Wu,Biao Liu,Jiahao Liu,Wei Xiang,Xing Hu,Xuewen Liu,Jianfei Xie,Jin Tang,Zhi Long,Long Wang,Hongliang Zeng,Jianye Liu 한국생체재료학회 2022 생체재료학회지 Vol.26 No.1

Background: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. Methods: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. Results: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/sicircPRMT5 regulated the miR-30c/SNAIL1/E-adherin axis, inhibiting bladder cancer growth and progression. Conclusion: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer.

Study of the Electric Field Screening Effect for Field Emitter Arrays

Nannan Li,Fei Yan,Shucai Pang,Lei Chen,Dazhi Jin,Wei Xiang,De Zhang,Jingyi Dai,Baoqing Zeng 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.8

Understanding the influence of the interaction of emitters on the field emission performance of a field emitter array is of great importance for the design of a multi-emitter device. In this work, a screening factor S is defined to quantify the effect of electric field screening due to the interaction of emitters in an array. A three-dimensional finite integration technique is used to study the electric field screening effect for Spindt-type field emitter arrays which have potential applications in flatscreen displays and high-power vacuum electronics. The dependences of the electric field screening on the tip-to-tip spacing, half-angle of the emitter, height of the emitter and radius of the tip’s curvature are analyzed. The effects of the variations in the size of the emitter’s geometry on the most efficient arrangement to achieve maximum emission efficiency in an array are discussed.