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Pan Jin,Fuqiang Yin,Li Huang,Li Zheng,Jinmin Zhao,XINGDONG ZHANG 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Neuro-osteological interactions have an important role in the regulation of bone metabolism and regeneration. Neuropeptides combined with porous biphasic calcium phosphates (BCP) using protein adsorption may contribute to the acceleration of bone formation. In the present study, we investigated the effect of BCP combined with nerve growth factor (NGF) on the growth of osteoblasts in vitro and the combinational therapeutic effect on the repair of calvarial defects in vivo. NGF was separated and purified from Chinese cobra venom using a simplified three-step chromatography method. BCP combined with NGF exerted a potent effect on osteoblast differentiation, as evidenced by enhanced cell proliferation, increased ALP activity and the upregulated expression of osteogenesis-related genes and proteins. Further, combinational therapy with BCP and NGF improved calvarial regeneration, which was superior to treatment with therapy alone, as observed using imageological and morphological examination and histological and immunohistochemical staining. The results confirmed the effect of neuro-osteological interactions through combinatorial treatment with NGF and BCP to promote osteogenesis and bone formation, which may provide an effective and economical strategy for clinical application.
APPLICATIONS OF CALCIUM PHOSPHATE NANOPARTICLES IN POROUS HARD TISSUE ENGINEERING SCAFFOLDS
ZHE WANG,ZHURONG TANG,FANGZHU QING,YOULIANG HONG,XINGDONG ZHANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.4
To repair bone defects, an important approach is to fabricate tissue engineering sca®olds as substitutions to replace auto-/allologous bones. Currently, processing a biomaterial into threedimensional porous sca®olds and incorporating the calcium phosphate (Ca?P) nanoparticles into sca®olds pro¯le two main characteristics of bone tissue engineering sca®olds. Based on this fact, in this paper we describe the design principles of the Ca?P nanoparticle-based and porous bone tissue engineering sca®olds. Then we summarize a variety of the Ca?P nanoparticle-based sca®olds, including discussion of the integration of the Ca?P nanoparticles with ceramics and polymers, followed by introduction of safety of the Ca?P nanoparticles in scaffolds.