Modified apolipoprotein (apo) A-I by artificial sweetener causes severe premature cellular senescence and atherosclerosis with impairment of functional and structural properties of apoA-I in lipid-free and lipid-bound state

Jang, Wookju,Jeoung, Nam Ho,Cho, Kyung-Hyun Springer-Verlag 2011 Molecules and cells Vol.31 No.5

Modified Apolipoprotein (apo) A-I by Artificial Sweetener Causes Severe Premature Cellular Senescence and Atherosclerosis with Impairment of Functional and Structural Properties of apoA-I in Lipid-Free and Lipid-Bound State

Wookju Jang,정남호,조경현 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.5

Long-term consumption of artificial sweeteners (AS) has been the recent focus of safety concerns. However, the potential risk of the AS in cardiovascular disease and lipoprotein metabolism has not been investigated sufficiently. We compared the influence of AS (aspartame, acesulfame K, and saccharin) and fructose in terms of functional and structural correlations of apolipoprotein (apo) A-I and high-density lipoproteins (HDL), which have atheroprotective effects. Long-term treatment of apoA-I with the sweetener at physiological concentration (3 mM for 168 h) resulted in loss of antioxidant and phospholipid binding activities with modification of secondary structure. The AS treated apoA-I exhibited proteolytic cleavage to produce 26 kDa-fragment. They showed pro-atherogenic properties in acetylated LDL phagocytosis of macrophages. Each sweetener alone or sweetener-treated apoA-I caused accelerated senescence in human dermal fibroblasts. These results suggest that long-term consumption of AS might accelerate atherosclerosis and senescence via impairment of function and structure of apoA-I and HDL.

Chemical constituents from Hibiscus hamabo and their antiphotoaging effects on UVA-induced CCD-986sk

Park Gwee Kyo,Jang Wookju,Kim Bo Yun,Oh Kyung‐Eon,Kim You Ah.,Kwon Hyuk Joon,Kim Soo‐Young,Park Byoung Jun 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.5

To investigate the phytochemical constituents of Hibiscus hamabo, a chromatographic method was used. A new phenolic glucoside, woodorien A (1), along with 10 known compounds, rutin (2), isoquercetin (3), nicotiflorin (4), astragalin (5), trans-tiliroside (6), N-trans-feruloyltyramine (7), N-trans-caffeoyltyramine (8), woodorien (9), quercetin 30-galactopyranoside (10), and cis-tiliroside (11), was isolated. The structure of 1 was identified as ethyl 3-O-β-D-glucopyranosyl- 4-hydroxybenzoate. Compounds 8, 9, and 11 were isolated from genus Hibiscus, and 2–7 and 10 were isolated from H. hamabo for the first time. Their chemical structures were determined through the interpretation of spectroscopic data. The properties of the UVA photoaging induction assay of all isolated compounds were measured. Compounds 3, 8, and 10 exhibited recovery from photoaging induced by UVA in CCD-986sk. These results suggest that H. hamabo is a potential source of natural antiphotoaging ingredients and can be used in cosmetic applications.

핑크-알로에의 항산화, NO 생성 억제 및 세포 재생 효과

이경주 ( Kyung Ju Lee ),장욱주 ( Wookju Jang ),김유아 ( You Ah Kim ),박병준 ( Byoung Jun Park ),강학희 ( Hakhee Kang ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.3

알로에 베라(Aloe barbadensis Miller)는 예로부터 화상이나 상처, 습진 등의 다양한 피부 질환을 개선하기 위해 사용해 왔다. 이러한 효능은 알로에가 가지고 있는 비타민, 무기질, 당, 페놀 물질, 지방산. 아미노산 등의 다양한 성분에 의한 것으로 보고가 되었다. 알로에는 극한의 환경에서 스스로를 보호하기 위해서 성분을 변화시키며, 이로 인해 색깔이 녹색에서 적색으로 변화한다. Aloe-emodin은 알로에속 식물의 대표적인 페놀성 물질로 항산화 및 항염 활성을 나타낸다. 그러나 지금까지 알로에의 활성 물질인 알로에-에모딘을 증가시키고 알로에의 색상을 변화시키는 공정 개발에 대한 연구는 보고되지 않았다. 따라서 본 연구의 목적은 aloe-emodin 함량을 증가시키고 알로에의 항산화 및 항염 활성을 증대시키는 핑크 알로에 제조공정을 개발하는 것이다. 이를 위해 알로에를 적정 조건으로 가온한 결과 핑크 알로에는 일반 알로에 대비 aloe-emodin 함량이 증가하였으며, 항산화 활성 증대, NO 생성 억제, 세포 재생 촉진 등의 효과를 나타내었다. 본 연구를 통해 화장품 분야에서 핑크 알로에의 신규 항노화 소재로 활용성을 확인하였다. Aloe vera (Aloe barbadensis Miller) has been used since ancient times to improve various skin diseases such as burns, wounds, and eczema. It has been reported that Aloe vera contains vitamin, enzyme, mineral, sugar, phenolic compound, fatty acid and amino acid. Aloe vera changes its color from green to red under the extreme thermal and arid climate to protect itself. These morphological changes induce variation of composition such as increasing of aloe-emodin content. Aloe-emodin is one of the major anthraquinone in aloe family plants. Since aloe-emodin contains a polyphenolic structure, this compound may be responsible for the reported antioxidant and anti-inflammatory effects of aloe. However, there is no research on the process of increasing the compounds of Aloe vera. Therefore, the purpose of this study is to develop a pink aloe manufacturing process that increases the aloe-emodin content and enhances the antioxidant and anti-inflammatory activities of aloe. As a result of heating aloe under appropriate conditions, pink aloe increased aloe-emodin content compared to general aloe, and exhibited effects such as increasing antioxidant activity, inhibiting NO production, and promoting cell regeneration. Through this study, the applicability of pink aloe as a new anti-aging material in the cosmetic field was confirmed.

Apolipoprotein A-IV is a novel substrate for matrix metalloproteinases.

Park, Ji Yoon,Park, Jun Hyoung,Jang, Wookju,Hwang, In-Kwan,Kim, In Ja,Kim, Hwa-Jung,Cho, Kyung-Hyun,Lee, Seung-Taek Japanese Biochemical Society 2012 The Journal of biochemistry Vol.151 No.3

<P>Screening of matrix metalloproteinase (MMP)-14 substrates in human plasma using a proteomics approach previously identified apolipoprotein A-IV (apoA-IV) as a novel substrate for MMP-14. Here, we show that among the tested MMPs, purified apoA-IV is most susceptible to cleavage by MMP-7, and that apoA-IV in plasma can be cleaved more efficiently by MMP-7 than MMP-14. Purified recombinant apoA-IV (44-kDa) was cleaved by MMP-7 into several fragments of 41, 32, 29, 27, 24, 22 and 19 kDa. N-terminal sequencing of the fragments identified two internal cleavage sites for MMP-7 in the apoA-IV sequence, between Glu(185) and Leu(186), and between Glu(262) and Leu(263). The cleavage of lipid-bound apoA-IV by MMP-7 was less efficient than that of lipid-free apoA-IV. Further, MMP-7-mediated cleavage of apoA-IV resulted in a rapid loss of its intrinsic anti-oxidant activity. Based on the fact that apoA-IV plays important roles in lipid metabolism and possesses anti-oxidant activity, we suggest that cleavage of lipid-free apoA-IV by MMP-7 has pathological implications in the development of hyperlipidemia and atherosclerosis.</P>

Rapid detection for modified HLD to prognostic diagnosis

Jaesool Shim,Kyung-Hyun Cho,Wookju Jang,Gyungdong Lee 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.4

아쿠아포닉스 기술을 이용한 친환경 항노화 제품 개발

김유아 ( You Ah Kim ),전태병 ( Tae Byeong Jeon ),장욱주 ( Wookju Jang ),박병준 ( Byoung Jun Park ),강학희 ( Hakhee Kang ) 대한화장품학회 2019 대한화장품학회지 Vol.45 No.3

본 연구에서는 국내 자생식물로부터 지속가능한 천연 항노화 화장품 신소재 개발을 위해 수생식물인 어리연꽃의 친환경 아쿠아포닉스 시스템 적용 가능성 및 항노화 효능을 검증하였다. 어리연꽃은 아쿠아포닉스 시스템내에서 부엽형태의 담액식 방식에서 성장 가능하고, 근경번식을 통해 증식됨이 확인되었다. 또한, 어리연꽃의 재배에 질산염, 칼륨 및 수온이 크게 영향을 미치며, 최적 조건은 각각 80 μg/mL, 63.5 μg/mL 및 25 ℃로 확인되었다. 특히 주성분 2종(3,7-di-O-methylquercetin-4’-O-β-glucoside 및 sweroside)이 약 5 μg/mL 이상 함께 존재 시 시너지 유효성이 있었다. 어리연꽃 추출물은 환경 유해물질 benzo[α]pyrene, ammonium nitrate, formaldehyde에 의해 손상된 피부 세포의 회복에 유의미한 효과가 있으며, 염증성 조절인자인 PGE₂, TNF-α 및 COX-2를 억제하고, 콜라겐 분해 효소인 MMP-1의 생성을 억제함으로써 항염 및 항노화 효과가 있음이 확인되었다. 따라서 아쿠아포닉스 기술 기반 어리연꽃 추출물의 원료 표준화는 화장품 분야에서 신규 항노화 기능성 소재로서의 활용 가능성이 있을 것으로 사료된다. To develop sustainable new natural anti-aging ingredients from Korean native plants, we investigated the cultivation potential of Nymphoides indica using the eco-friendly aquaponics system, and tested the anti-aging effects from N. indica extracts. N. indica could be grown in aquaponics system using floating leaved deep water culture method, and propagated through rhizome propagation. It was confirmed that the nitrate (80 μg/mL), potassium (63.5 μg/mL) and water temperature (25 ℃) greatly affected the cultivation of the N. indica. In addition, synergistic effects were found when two major components (3,7-di-O-methylquercetin-4’-O-β-glucoside & sweroside) were present at more than about 5 μg/mL. The extract had a significant effect on the recovery of skin cells damaged by environmental pollutant such as benzo[α]pyrene, ammonium nitrate, formaldehyde. It also suppressed PGE₂, TNF-α and COX-2, and inhibited the production of MMP-1. Taken together, the results suggested that the standardized extracts of N. indica cultivated in the aquaponics has considerable potential as a new cosmetics ingredient with an anti-aging effect.

Effects of Raphanus sativus var. niger (Black Radish) Fermented with Lactobacillus plantarum on Alcohol Metabolism in Rats

강경구,Seo Min-Soo,Sung Soo-Eun,Choi Joo-Hee,Lee Si-Joon,Kim KilSoo,Jang Wookju,Lee Hak Sung 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.2

Excessive drinking causes various side effects including hepatic and neurological diseases that can lead to serious social problems, and thus, efforts to search for a way to help to promote alcohol decomposition are increasingly needed. Raphanus sativus var. niger commonly known as black radish has been reported to have biologically active glycosides such as glucosinolates and also known to improve liver functions, suggesting that this vegetable may have an action to promote alcohol metabolism. In the present study, this possibility was tested with an extract prepared from the black radish fermented with Lactobacillus plantarum in rats. The fermented black radish extract (75 or 300 mg/kg) or saline (2 mL/kg) was administered to rats orally. After 30 minutes, ethanol (4 g/kg) was administered orally using 25% ethanol in water and blood was sampled at 0, 1, 2 and 4 hours after ethanol administration and then euthanasia was performed to get the liver. The sera obtained from the blood samples were used for the concentration analysis of ethanol and acetaldehyde and the examination of blood chemistries. The liver tissues were used for the activity assays for alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). It was observed that in the rats administered the fermented black radish extract, the concentrations of ethanol and acetaldehyde decreased more and simultaneously the activities of ADH and ALDH increased more than those in the saline-treated rats. Any significant changes in the blood chemistries and histology of liver tissues were not observed. These results suggest that the fermented black radish extract has a potential that may ameliorate hangover symptoms caused by excessive alcohol ingestion.