http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
역전사 (逆轉寫) 중합효소연쇄반응 (重合酵素連鎖反應) ( RT-PCR ) 과 제한효소 분석을 이용한 오이 모자이크 바이러스의 신속한 검정 및 동정
박원목(Won Mok Park),유기현(Ki Hyun Ryu),김수중(Su Joong Kim),최장경(Jang Kyung Choi) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.3
Based upon the nucleotide sequence of As strain of cucumber mosaic virus (CMV-As) RNA4, coat protein (CP) gene was selected for the design of oligonucleotide primers of polymerase chain reaction (PCR) for detection and identification of the virus. Reverse transcription and polymerase chain reaction (RT-PCR) was performed with a set of 18-mer CMV CP-specific primers to amplify a 671 bp fragment from crude nucleic acid extracts of virus-infected leaf tissues as well as purified viral RNAs. The minimum concentrations of template viral RNA and crude nucleic acids from infected tobacco tissue required to detect the virus were 1.0 fg and 1 : 65,536 (w/v), respectively. no PCR product was obtained when potato virus Y-VN RNA or extracts of healthy plants were used as templates in RT-PCR using the same primers. The RT-PCR detected CMV-Y strain as well as CMV-As strain. Restriction analysis of the two individual PCR amplified DNA fragments from CMV-As and CMV-Y strains showed distinct polymorphic patterns. PCR product from CMV-As has a single recognition site for EcoRI and EcoRV, respectively, and the product from CMV-Y has no site for EcoRI or EcoRV but only one site for HindIII. The RT-PCR was able to detect the virus in the tissues of infected pepper, tomato and Chinese cabbage plants.