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안개초에서 분리한 Cucumber mosaic virus의 특성
박호섭 ( Ho Seop Park ),최장경 ( Jang Kyung Choi ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2009 강원 농업생명환경연구 Vol.21 No.-
An isolate of Cucumber mosaic virus (CMV) was isolated from Gypsophila paniculata showing mosaic symptoms, and designated as Gyp-CMV. Gyp-CMV was characterized by host reaction on several indicator plants, RT-PCR analysis, restriction enzyme profile of the PCR products and amino acid sequence analysis of coat protein. Gyp-CMV differed markedly in their host reaction to Fny-CMV on Cucumis sativus and Gomphrena globosa. In the Cucumis sativus and Gomphrena globosa Fny-CMV induced typical mosaic symptoms, whereas symptoms induced by Gyp-CMV was developed symptomless infection in the plants. In experiments of dsRNA analysis, RT-PCR and RFLP profiles, properties Gyp-CMV was come within subgroup IA CMVs. Gyp-CMV was highly similar to Fny-CMV in homology of amino acid sequence of coat protein, and only one amino acid change was observed.
Cucumber mosaic virus의 약독변이계통 Pa/m의 특성과 외피단백질 유전자의 염기서열 분석
이유리 ( You Ri Lee ),최장경 ( Jang Kyung Choi ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2009 강원 농업생명환경연구 Vol.21 No.-
An attenuated mutant of Cucumber mosaic virus (CMV), CMV-Pa/m, originated from CMV-Pa was characterized by host reaction on several indicator plants, RT-PCR analysis, restriction enzyme profile of the PCR products and amino acid sequence analysis of coat protein. CMV-Pa/m differed markedly in their host reaction to CMV-Pa or CMV-Fny on Nicotiana spp. and Capsicum annuum. In the Nicotiana spp. and Capsicum annuum plants, CMV-Pa and CMV-Fny induced typical mosaic symptoms. However, symptoms induced by CMV-Pa/m was developed mild mosaic or symptomless infection in the plants. In experiments of RT-PCR and RFLP profiles, properties CMV-Pa/m was come within subgroup IA CMVs. CMV-Pa/m was highly similar to CMV-Pa in homology of amino acid sequence of coat protein, and only one amino acid change was observed.