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노환국,이장형,Nho, Whan-Gook,Lee, Jang-Hyung 국립한국농수산대학교 교육개발센터 2004 현장농업연구지 = Journal of practical agricultural resear Vol.6 No.1
The method of insertion of T-type cannula into the proximal duodenum of cattle was established for the feed digestibility test. Five cattle were anesthetized with rumpun and 2% lidocaine. The incision(15 ~ 20 cm) through the abdominal wall exposing the peritoneal cavity was made. The identified duodenum was extracted through the abdominal incision. The cannula was inserted into the incised duodenal wall. Cannula barrel was extracted between the 10th and 11th rib. All of operated cattle were healthy and cannula remained completely functional until 20 months after a proximal duodenal cannulation.
김완영,노환국,Kim, Wan-Young,Nho, Whan-Gook 국립한국농수산대학교 교육개발센터 2004 현장농업연구지 = Journal of practical agricultural resear Vol.6 No.1
Se is essential for a number of enzymes that perform important metabolic functions necessary for good health. However, people in many countries do not appear to consume adequate amounts of Se to support the maximal expression of the selenoproteins and Se retention in the body of animals and humans is dependent on the ingested Se source such as organic and inorganic Se. Therefore, this review was discussed to explore metabolic characterization regarding intestinal absorption, bioavailability and selenoprotein synthesis according to animal species such as monogastrics including human beings and ruminants. Generally, organic Se provided to animals is more effective than inorganic Se in body retention for the animal owing to the difference of manner for intestinal absorption. But, Se absorption in ruminants depending on its chemical form still remained questioned by several microbial actions and feeding regimen in the rumen. And Se absorbed through small intestine is utilized for the synthesis of selenoproteins and/or retained as selenoamino acids in the body. Retained Se in the body may be recycled to synthesize selenoproteins as lacked of dietary Se. In conclusion, desirable forms of Se ingestion in the animal may be useful for Se fortification in animal products as well as well being for humans and animals.
Comparative immunohistochemical characterization of canine seminomas and Sertoli cell tumors
Chi-Ho Yu,Du-Na Hwang,이지영,김종혁,Keum-Soon Im,Whan-Gook Nho,Young-Soo Lyoo,서정향 대한수의학회 2009 JOURNAL OF VETERINARY SCIENCE Vol.10 No.1
Primary testicular tumors are the most common causes of cancer in male dogs. Overall, the majority of canine patients should be cured by testicular surgery. However, tumor markers are not well-known in veterinary medicine. We sought to determine using immunohistochemistry whether the combined human testicular tumor markers (placental alkaline phosphatase, OCT3/4, CD30, alpha-fetoprotein, inhibin-alpha, vimentin, c-KIT, and desmin) are expressed in canine seminomas and Sertoli cell tumors (SCTs). We examined 35 canine testicular tumors, 20 seminomas and 15 SCTs. c-KIT was expressed markedly in canine seminomas. Both inhibin-alpha and vimentin were expressed significantly in canine SCTs. The results of this study demonstrate differences and similarities between tumor marker expression of testicular tumors in dogs and humans. All the main markers in current routine use are discussed as well as potential useful markers for benign and malignant tumors, and tumor progression.
양재혁,박용수,노환국,손원근,이두식,황규계,임윤규,Yang, Jaehyuk,Park, Yong-Soo,Nho, Whan-Gook,Son, Won-Geun,Lee, Du-Sik,Hwang, Kyu-Kye,Lim, Yoon-Kyu Korea National University of Agriculture and Fishe 2014 현장농업연구지 = Journal of practical agricultural resear Vol.16 No.1
Equine herpesvirus type 1 (EHV-1) is an economically important pathogen of horses and exerts its major impact by inducing abortion storms and sporadic abortions in pregnant mares, early neonatal death in foals, and respiratory disease in young horses. Although equine herpesvirus type 4 (EHV-4) rarely causes clinical manifestations of disease in organs other than the respiratory track, isolated cases of myeloencephalopathy and sporadic abortions have been reported in EHV-4 infections. Here, we report an abortion storm in Thoroughbred breeding farms in Jeju island, South Korea. It occurred for 16 days from first abortion to last one. There were no clinical signs prior to abortion and stillbirth in broodmares. Two PCR-primers were made on glycoprotein B gene of EHV-1 and EHV-4 to amplify specific common regions of the viruses. We could detect the virus specific genes in aborted samples by PCR, and concluded that the cause of abortion storm was EHV infection. This report describes the first abortion storm case caused by EHV in Thoroughbred breeding farms in South Korea.
( Dong Hua Li ),( Young Kyoon Oh ),( Sang Rak Lee ),( Seong Ho Choi ),( Ok Ji Un ),( Yong Joo Seol ),( Whan Gook Nho ),( Sang Ho Moon ),( Kyoung Hoon Kim ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.6
The objectives of experiments were to compare directly the effects of synchrony of slowly fermented(corn and corn gluten meal; C-CGM) and of rapidly fermented ingredients(barley and soybean meal; B-SBM) in the rumen on starch disappearances in gastrointestinal tracts(Experiment 1) and growth performance(Experiment 2) of Hanwoo steers in the feedlot barn. In experiment 1, four Hanwoo steers(288±21kg) fitted with ruminal and "T" shaped duodenal cannula were placed in one pen with Calan gate and assigned randomly to a duplicate 2×2 Latin square design. In experiment 2, eight intact Hanwoo steers(311 ±8kg) were assigned randomly to one of two pens with Calan gate to evaluate the effect of the same diets as like in experiment 1 on growth performance. There were no differences in ruminal pH, ammonia and total VFA concentrations between treatments. Percentage of apparent ruminal starch disappearance was 33.3% unit lower(p<0.05) for steers fed C-CGM than for steers fed B-SBM diets and this difference resulted in 268% higher(p<0.05) in duodenal starch flows for steers fed C-CGM diet than for steers fed B-SMB diet. There was significant increase(p<0.05) in quantity(927 vs. 400 g/d) of corn starch digested post-ruminally compared to barley starch. However, percentage of starch apparently digested post-ruminally was 8% higher(p=0.1) in steers fed fast synchrony diet with B-SBM than in steers fed slow synchrony diets with C-CGM. The differences of percentage and amount of starch apparently digested post-ruminally between C-CGM and B-SBM diets did not affect rice straw DM intake, average daily gain(ADG) and feed efficiency. In conclusion, there is some uncertainty in regards to the relationship between site of starch digestion and DM intake, ADG, and feed efficiency in this study.
Development of Isolators for Gnotobiotic Miniature Pig
Young Ah Lee,Yoon Ju Cho,Jong-In Kim,Jae Won Lee,Byeong Han Lee,Whan Gook Nho,Jin Soo Han 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.3
Miniature pigs have been marked as a source of providing organs for xenotransplantation because they are similar with humans in the physiological structure, organ size and physiological state. Though the demands for gnotobiotic pigs are increasing by the rise of bio-organic researches, there are currently few facilities for producing miniature pigs gnotobiotically. Therefore the isolator only for the miniature pigs is essential for a production and study of the gnotobiotic miniature pigs. By utilizing the isolator for gnotobiotic miniature pigs, caretakers don't have to take complicated sterilization or pasteurization steps, and thus it reduces the risk of contamination. And it is further expected to have economically positive effects by reducing costs compared to a small scaled gnotobiotic breeding facility. We developed two models of isolator and applied to patents. Weighing balance and partition were installed in the isolator to perform the body weight and temperature measurement, injection and blood-collection more comfortably without any contamination, and it could minimize the stress of animal. It is easy and comfortable to handle miniature pigs using these isolators and expected to be very useful for the study of gnotobiotic pigs.
오제스키병의 생체 조기진단을 위한 면역세포화학, In situ hybridization 및 전자현미경적 연구
문운경,김순복,서정향,송근석,노환국,Moon, Oun-kyong,Kim, Soon-bok,Sur, Jung-hyang,Song, Geun-suk,Nho, Whan-gook 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
The purpose of this study was to establish early diagnostic methods for the detection of Aujeszky's disease viral antigens and nucleic acid in nasal cells, and buffy coats from experimentally infected living pigs by a combination of immunocytochemistry, in situ hybridization with digoxigenin(DIG)-labled probe and electron microscopy. Forty days old piglets were inoculated intranasally with $10^{7.0}TCID_{50}$ of Aujeszky's disease virus (ADV, NYJ-1-87 strain). The viral antigens and nucleic acid of ADV were detected in nasal cells, and buffy coat for 20 days after inoculation by immunocytochemistry, in situ hybridization with DIG-labeled probe and electron microscopical method. The results were compared with conventional methods such as a porcine Aujeszky's disease serodiagnostic(PAD) kit, neutralization test(NT) and virus isolation. 1. The viral antigens, nucleic acids and capsids of ADV were detected in nasal cells, buffy coats from 3 days to 20 days after inoculation by immunocytochemistry, in situ hybridization with DIG-labeled probe and electron microscopy, respectively. 2. When viral antigens were detected by the immunocytochemical technique, a diffuse brown deposit was observed in the nucleus and cytoplasm of nasal cells, buffy coats and PK-15 cells under a microscope. 3. DIG-labeled DNA probe was prepared by amplification of conserved sequence of recombinant ADV-gp50 clone with polymerase chain reacction. When ADV-DNA was detected by ISH with DIG-labeled probe, purplish blue pigmentation were observed in the nuclei and cytoplasms of ADV-infected cells under a microscope. Positive signals were observed in nasal cells and in the buffy coat and PK-15 cells at the first day after inoculation. 4. Where ADV-capsids were detected by transmission electron microscopical method, aggregation of capsids was observed in the nuclei and cytoplasms of nasal cells, buffy coats and PK-15 cells. The results suggested that these methods were considered as the highly sensitive and reliable tools for rapid and confirmative diagnosis of Aujeszky's disease in living pigs.