http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Wenjing Han (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Expression of fox-related genes in the skin follicles of Inner Mongolia cashmere goat
Wenjing Han,Xiaoyan Li,Lele Wang,Honghao Wang,Kun Yang,Zhixin Wang,Ruijun Wang,Rui Su,Zhihong Liu,Yanhong Zhao,Yanjun Zhang,Jinquan Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3
Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.
-
BRG1 Promotes chromatin remodeling around DNA damage sites
Wenjing Qi,Hongyu Chen,Chengwen Lu,Qingpan Bu,Xiaoguang Wang,Liping Han 한국통합생물학회 2018 Animal cells and systems Vol.22 No.6
Chromatin remodeling complexes play important roles in various DNA metabolism processes, including DNA damage repair. BRG1 is the core subunit of the SWI/SNF complex, which plays critical roles in cell cycle regulation, cell development, cell differentiation, and tumorigenesis. In the present study, we report that BRG1 depletion increased the percentage of apoptotic cells in etoposide-treated cells. Moreover, western blotting and immunofluorescence data showed that BRG1 depletion decreased H2AX phosphorylation and caused defective phosphorylated histone H2AX (γH2AX) clearance. Furthermore, we found that in both SW13 and U2OS cells, BRG1 expression could increase the sensitivity of genomic DNA to micrococcal nuclease (MNase) and facilitate chromatin relaxation around DNA damage sites. Thus, the results provide evidence that BRG1 plays an important role in early DNA damage repair by remodeling the chromatin structure near DNA damage sites.
-
Wenjing Xu,Zhanggen Huang,Qianqian Guo,Yaqin Hou,Xiaojin Han 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.5
The SO2 oxidation over V2O5/AC catalyst was studied using an in situ diffuse reflectance infrared Fouriertransformation spectroscopy technique at 120 oC. Results reveal that the surface oxygen groups could neither act asactive sites for SO2 oxidation nor supply the oxygen needed for VV↔VIV redox cycle. The vanadia species and gasphase oxygen are essential for SO2 oxidation. During SO2 oxidation over V2O5/AC, the surface hydroxyl groups involvein the formation of sulfate species. The role of water vapor in flue gas might be to supplement the hydroxyl groupsconsumed so that the SO2 oxidation could continue.
-
Xiujuan Tian,Wenjing Qi,Hongyu Chen,Xianlu Zeng,Liping Han,Donghui Mi 한국통합생물학회 2016 Animal cells and systems Vol.20 No.5
In pre-initiation complexes, RNA helicase A interacts with β-actin and acts as a bridging factor linking nuclear actin with RNA polymerase II (Pol II). In addition, β-actin participates in Pol IIdependent transcription elongation by interacting with the positive transcription elongation factor Cdk9. However, many relationships between β-actin and Pol II remain to be identified. In an interleukin 6 (IL-6)-induced p21 expression model, we demonstrated that β-actin knockdown reduced p21 expression. Immunofluorescence analysis showed that the colocalization of β-actin and Pol II increased significantly in cells treated with IL-6. It is known that the Rpb5, Rpb6 and Rpb7 subunits are located at the surface of the enzyme. We next constructed recombinant pcDNA-HA-Rpb5, pcDNA-HA-Rpb6 and pcDNA-HA-Rpb7 plasmids and expressed the three polymerase II subunits in HepG2 cells. We found that β-actin could be immunoprecipitated with HA-Rpb5 and HA-Rpb7. A Glutathione-S-transferase pull-down assay revealed that β-actin was associated with Rpb5 and Rpb7 in vitro. Furthermore, overexpression of Rpb5 and Rpb7 in cells reduced p21 expression significantly, suggesting that Rpb5 and Rpb7 competitively interact with β-actin. This study shows that β-actin associates with Pol II subunits through direct proteinprotein interactions and provides fundamental insight into Pol II transcriptional regulation.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
