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Wang, Shao-Kang,Yang, Lei,Wang, Ting-Ting,Huang, Gui-Ling,Yang, Li-Gang,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
Esophageal cancer is a common malignant tumor occurring in human esophageal epithelial tissue. The primary purpose of this paper was to define the effects of ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$, alone and in combination, on cell proliferation, cell cycle and apoptosis of human esophageal cancer EC9706 cells. Treatment with different concentrations of ${\beta}$-carotene and/or 1,25-dihydroxyvitamin $D_3$. MTT assay showed that ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$ significantly inhibited proliferation of EC9706 cells in a dose- and time-dependent manner. Further studies also demonstrated that ${\beta}$-carotene alone or 1,25-dihydroxyvitamin $D_3$ alone caused a marked increase on the induction of apoptosis in EC9706 cells. The percentage of G0/G1-phase cells significantly increased on addition of 1,25-dihydroxyvitamin $D_3$ alone, but there were no significant changes with ${\beta}$-carotene alone. These two agents in combination synergistically inhibited cell growth and induced apoptosis. Therefore, our results indicate that ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$ in combination may provide a novel strategy for preventing and treating esophageal cancer.
Wang, Ting-Ting,Wang, Shao-Kang,Huang, Gui-Ling,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an $IC_{50}$ of $70.7{\pm}1.72{\mu}M$ at 24h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.
Wang, Xin-kui,Yan, Shi-run,Li, Zhen-hua,Fan, Kang-nian,Kang, Mao-qing,Peng, Shao-yi 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.2
A novel non-phosgene process for the synthesis of methyl N-phenyl carbamate (MPC) by a reaction of phenylurea with methanol was studied. The reaction between phenylurea and methanol was found to be a spontaneous reaction that took place in the absence of catalyst and gave MPC as the main product. Addition of a catalyst markedly influenced the reaction behavior. A basic catalyst greatly enhanced the yield of MPC, whereas an acidic catalyst promoted the formation of aniline and methyl carbamate. Moderate strength of basicity showed the best catalytic performance in the cases studied. The mechanism of reaction and catalyst functioning was discussed.
Dispersion of carbon nanotubes in aluminum improves radiation resistance
So, Kang Pyo,Chen, Di,Kushima, Akihiro,Li, Mingda,Kim, Sangtae,Yang, Yang,Wang, Ziqiang,Park, Jong Gil,Lee, Young Hee,Gonzalez, Rafael I.,Kiwi, Miguel,Bringa, Eduardo M.,Shao, Lin,Li, Ju unknown 2016 Nano energy Vol.22 No.-
<P><B>Abstract</B></P> <P>We can mass-produce metal/carbon nanotube (CNT) composites that show improved radiation tolerance. The 0.5wt% Al+CNT composite showed improved tensile strength without reduction of tensile ductility before radiation, and reduced void/pore generation and radiation embrittlement at high displacements per atom (DPA). Under helium ion irradiation up to 72DPA, the 1D carbon nanostructures survive, while sp<SUP>2</SUP> bonded graphene transforms to sp<SUP>3</SUP> tetrahedral amorphous carbon. Self-ion (Al) irradiation converts CNTs to a metastable form of Al<SUB>4</SUB>C<SUB>3</SUB>, but still as slender 1D nanorods with prolific internal interfaces that catalyze recombination of radiation defects, reducing radiation hardening and porosity generation. The 1D fillers may also form percolating paths of “nano-chimneys” that outgas the accumulated helium and other fission gases, providing an essential solution to the gas accumulation problem.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Dispersion of CNTs showed improved tensile strength without reduction of ductility. </LI> <LI> CNTs in Al reduced void/pore generation and radiation embrittlement at high DPA. </LI> <LI> Under He ion irradiation, 1D CNTs survive, while sp<SUP>2</SUP> bond transform to sp<SUP>3</SUP> carbon. </LI> <LI> Formation of metastable 1D Al<SUB>4</SUB>C<SUB>3</SUB> from CNTs still recombine radiation defects. </LI> <LI> This nanocomposite can help improve bulk properties for nuclear applications. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3
BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.
Guangdong Hu,Jing Wang,Hui Huang,Fusheng Quan,Jian Kang,Yongyan Wu,Yuanpeng Gao,Feng Su,Minghao Shao,Yong Zhang 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2
Transposons are widely used for geneticengineering in various model organisms. Recently, piggyBac(PB) has been developed as a transposable and efficientgene transfer tool in mammalian cells. In the present study,we developed three types of PB transposon systemscontaining a dual plasmid system (DPS), a single plasmidsystem (SPS), and a DNA-mRNA combined system (DRPS)and characterized their basic properties in HEK293 cells. The basic elements of the donor plasmid included aselectable-reporter gene expression cassette, two loxP sitesin the same orientation, a multiple cloning site, and twochicken β-globin insulator core elements. We further identifiedthe function of the selectable-reporter and examined PBintegration sites in the human genome. Moreover, wecompared the transposition efficacy and found that SPStransposed more efficiently, as compared to DPS; integrationinto the host genome was determined by measuring PBaseactivity. Results discovered the loss of PBase activity in theDRPS, indicating that this system is much more biologicallysafe, as compared to DPS and SPS. Finally, we employedthe DRPS to successfully perform a gene delivery intobovine mammary epithelial cells (BMECs). Taken together,the information from this study will improve the flexibilityof PB transposon systems and reduce the genotoxicity ofPBase in genetic engineering.
Huang, Gui-Ling,Yang, Lei,Su, Ming,Wang, Shao-Kang,Yin, Hong,Wang, Jia-Sheng,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Objective: The aim was to evaluate roles of vitamin D3 (VD3) and beta-carotene (BC) in the development of esophageal squamous cell carcinoma (ESCC) in a high-risk area, Huai'an District, Huai'an City, China. Methods: 100 new ESCC diagnosed cases from 2007 to 2008 and 200 residency- age-, and sex-matched healthy controls were recruited. Data were collected from questionnaires, including a food frequency questionnaire (FFQ) to calculate the BC intake, and reversed phase high-performance liquid chromatography (RP-HPLC) was used to measure the serum concentrations of BC and VD3. Odds ratios (OR) and 95% confidence intervals (CI) were calculated in conditional logistic regression models. Results: The average dietary intake of BC was $3322.9{\mu}g$ (2032.4-5734.3) in the case group and $3626.8{\mu}g$ (1961.9-5827.9) in control group per capita per day with no significant difference by Wilcoxon test (p>0.05). However, the levels of VD3 and BC in the case group were significantly lower than in the control group (p<0.05). The OR values of the highest quartile and the lowest quartile of VD3 and BC in serum samples were both 0.13. Conclusion: Our results add to the evidence that high circulating levels of VD3 and BC are associated with a reduced risk of ESCC in this Chinese population.
Clinical Significance of SH2B1 Adaptor Protein Expression in Non-small Cell Lung Cancer
Zhang, Hang,Duan, Chao-Jun,Chen, Wei,Wang, Shao-Qiang,Zhang, Sheng-Kang,Dong, Shuo,Cheng, Yuan-Da,Zhang, Chun-Fang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
The SH2B1 adaptor protein is recruited to multiple ligand-activated receptor tyrosine kinases that play important role in the physiologic and pathologic features of many cancers. The purpose of this study was to assess SH2B1 expression and to explore its contribution to the non-small cell lung cancer (NSCLC). Methods: SH2B1 expression in 114 primary NSCLC tissue specimens was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome. Additionally, 15 paired NSCLC background tissues, 5 NSCLC cell lines and a normal HBE cell line were evaluated for SH2B1 expression by RT-PCR and immunoblotting, immunofluorescence being applied for the cell lines. Results: SH2B1 was found to be overexpressed in NSCLC tissues and NSCLC cell lines. More importantly, high SH2B1 expression was significantly associated with tumor grade, tumor size, clinical stage, lymph node metastasis, and recurrence respectively. Survival analysis demonstrated that patients with high SH2B1 expression had both poorer disease-free survival and overall survival than other patients. Multivariate Cox regression analysis revealed that SH2B1 overexpression was an independent prognostic factor for patients with NSCLC. Conclusions: Our findings suggest that the SH2B1 protein may contribute to the malignant progression of NSCLC and could offer a novel prognostic indicator for patients with NSCLC.
Sliding mode and predictive current control strategy of the three-phase Vienna rectifier
Feng, Xingtian,Tao, Yuanyuan,Cui, Xiao,Shao, Kang,Wang, Yubin The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.3
This study involves the design and implementation of a novel double closed-loop control of the three-phase Vienna rectifier. To achieve the unit power factor operation of the rectifier and reduce the harmonic content of the grid side, the double closed-loop control strategy is proposed on the basis of the voltage control of the outer loop and the current control of the inner loop. The voltage outer loop adopts the sliding-mode variable structure control strategy based on the improved reaching law; this strategy can effectively suppress switching jitter. The current inner loop adopts an improved predictive current control strategy that combines the traditional finite control set model predictive control (FCS-MPC) with the space vector modulation method (SVPWM), such that the optimal switching state obtained by the predictive controller is modulated to generate a switching signal. Moreover, the switching frequency can be controlled by adjusting the frequency of the carrier, and the switching frequency is fixed while preserving the advantages of model prediction control. The primary advantages of the proposed double closed-loop control strategy are as follows: (1) it does not require proportional-integral controllers in the current control loops, and (2) it keeps the output voltage stable. The Vienna rectifier simulation is performed on MATLAB. Then an experimental prototype based on SiC devices is developed to verify the excellent performance of the proposed strategy in steady and transient states.