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        Elucidating the effect of growth promoting endophytic fungus Piriformospora indica for seedling stage salinity tolerance in contrasting rice genotypes

        Reshna O. P.,Beena R.,Joy M.,Viji M. M.,Roy S. 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.5

        The growth and productivity of rice ( Oryza sativa L.) has been hampered by high soil salinity. Microbial endophytes, the most significant soil microorganisms, boost plant output per unit area by changing physiologically, and ecologically, allowing crop development in saline soils or climates with biotic and abiotic stresses. Piriformospora indica , an endophytic fungus, promotes plant development while also increasing plant resilience to environmental stresses such as salinity. Here we analyzed the beneficial effect of P. indica association on rice seedlings of both tolerant variety VTL-6 and susceptible variety Manu Ratna during different levels of salinity stress conditions (0, 100, 150, 200, 300 and 500 mM NaCl), to check the highest tolerating levels of salinity stress by P.indica in selected tolerant and susceptible rice genotypes. The growth parameters of rice seedlings such as shoot and root lengths, fresh and dry weights, seedling vigour index 1 and 2, root volume and root shoot ratio were found to be enhanced in P. indica -inoculated rice seedlings as compared to non-inoculated control seedlings, irrespective of whether they are exposed to salt stress or not. In the presence of the fungus, however, salt-stressed seedlings performed significantly better than non-inoculated control seedlings by maintain low Na + /K + ratio. All the growth parameters were decreased as the increase in salinity levels. The findings of this study revealed that this fungus has an important function in improving rice development under salt stress environments.

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        Exogenously applied plant growth regulator protects rice from heat-induced damage by modulating plant defense mechanism

        Lakshmi G.,Beena R.,Soni K. B.,Viji M. M.,Jha Uday Chand 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.1

        This study was undertaken to investigate the efect of biostimulants such as spermidine (1 mM, 2 mM), indole-3-acetic acid (10–3 M, 10–5 M), brassinolide (1 mg/L, 2 mg/L) and boron (50 mg/L, 100 mg/L) on protecting rice plants from heat-induced damage. Two rice genotypes, Nagina 22 (heat tolerant) and Manu Ratna (heat susceptible), primed with biostimulant solution were germinated, 14-day-old seedlings were further foliar sprayed with respective treatment solutions prior to the induction of heat stress (42 °C, 48 h). Heat stress-induced fatal damages to non-biostimulant-treated rice seedlings, whereas biostimulanttreated ones were protected from heat stress-induced damages, marked by lower levels of malondialdehyde (MDA), H2O2 coupled with increased levels of seedling vigour index (SVI), chlorophyll and proline contents. Results showed that 1 mM spermidine (Spd) and 10–5 M indole-3-acetic acid (IAA)-treated rice seedlings showed increased heat tolerance and were evaluated for its efect at reproductive stage stress in a pot culture study. At the panicle initiation stage, plants were pretreated with 1 mM Spd and 10–5 M IAA followed by the induction of heat stress, keeping it in a temperature-controlled polyhouse (42.1 °C, till the time of harvest). Spd- and IAA-pretreated heat-exposed plants exhibited yield improvement over control by altering physiological, biochemical and molecular mechanisms. Therefore, we conclude from our results that foliar IAA or Spd applications can be regarded as an agronomic strategy for mitigating the adverse efects of heat stress conditions on rice plants.

      • Synthesis and characterization of Rosuvastatin calcium impurity A; a HMG-CoA reductase inhibitor

        Lee, Y.H.,Viji, M.,Lee, E.,Jo, H.,Yoo, K.,Sim, J.,Lee, S.,Lee, K.,Lee, H.,Jung, J.K. Pergamon Press ; Elsevier Science Ltd 2017 Tetrahedron letters: the international organ for t Vol.58 No.26

        During the process development for multistep synthesis of Rosuvastatin calcium several impurities were obtained along with the final Rosuvastatin calcium. Out of this; synthesis of impurity A (acetone adduct) a minor impurity of Rosuvastatin calcium (3R,5S,6E)-7-[4-(4-fluorophenyl)-2-[[(2-hydroxy-2-methylpropyl)sulfonyl(methyl)amino]-6-(1-methylethyl)-pyrimidin-5-yl]-3,5-dihydroxyheptenoicacid hemicalcium salt, is described. The synthesis of impurity A has been accomplished in 6 steps; starting from formation of β-hydroxy sulfonamide as the key intermediate and followed by using convenient routes with overall yield of 13.5%. The target compound can be used as the reference substance of impurity of the Rosuvastatin calcium.

      • A novel cyclization/oxidation strategy for a two-step synthesis of (Z)-aurone

        Li, S.,Jin, F.,Viji, M.,Jo, H.,Sim, J.,Kim, H.S.,Lee, H.,Jung, J.K. Pergamon Press ; Elsevier Science Ltd 2017 Tetrahedron letters: the international organ for t Vol.58 No.14

        An efficient and facile two-step strategy for the synthesis of (Z)-aurone from arylacetylenes and salicyladehydes, via silver(I) nitrate mediated cyclization/oxidation in the presence of potassium carbonate has been developed. The key feature of our method was delicate cascade reaction, to provide the corresponding (Z)-aurone in high yield and good regio- and stereo selectivity.

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