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RISS 인기검색어
- 검색키워드
- 저자 : V. Sreenivasulu (검색결과 11 건)
- 무료
- 기관 내 무료
- 유료
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Sreenivasulu, M.,Gopal, D.V.R. Sai The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.1
Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus-P biotype (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector, sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein was over-expressed as a fusion protein in Escherichia coli. SDS-PAGE gel revealed that CP expressed as a ~40 kDa protein. The recombinant coat protein (rCP) fused with 6x His-tag was purified from E.coli using Ni-NTA resin. The antigenicity of the fusion protein was determined by western blot analysis using antibodies raised against purified PRSV. The purified rCP was used as an antigen to produce high titer PRSV specific polyclonal antiserum. The resulting antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) assay and compared its sensitivity levels with ELISA based assays for detection of PRSV isolates. IC-RT-PCR was shown to be the most sensitive test followed by dot-blot immunobinding assay (DBIA) and plate trapped ELISA.
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M. Sreenivasulu,D. V. R. Sai Gopal 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.1
Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus-P biotype (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector,sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein was over-expressed as a fusion protein in Escherichia coli. SDS-PAGE gel revealed that CP expressed as a ~40 kDa protein. The recombinant coat protein (rCP) fused with 6x His-tag was purified from E.coli using Ni-NTA resin. The antigenicity of the fusion protein was determined by western blot analysis using antibodies raised against purified PRSV. The purified rCP was used as an antigen to produce high titer PRSV specific polyclonal antiserum. The resulting antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (IC-RTPCR)assay and compared its sensitivity levels with ELISA based assays for detection of PRSV isolates. ICRT-PCR was shown to be the most sensitive test followed by dot-blot immunobinding assay (DBIA) and plate trapped ELISA.
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M. Sreenivasulu,D. V. R. Sai Gopal 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.2
Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus biotype-p (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector,sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein was over-expressed as a fusion protein in Escherichia coli. SDS-PAGE gel revealed that CP expressed as a ~40 kDa protein. The recombinant coat protein (rCP) fused with 6x His-tag was purified from E. coli using Ni-NTA resin. The antigenicity of the fusion protein was determined by western blot analysis using antibodies raised against purified PRSV. The purified rCP was used as an antigen to produce high titer PRSV specific polyclonal antiserum. The resulting antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (IC-RTPCR)assay and compared its sensitivity levels with ELISA based assays for detection of PRSV isolates. ICRT-PCR was shown to be the most sensitive test followed by dot-blot immunobinding assay (DBIA) and plate trapped ELISA.
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Spectroscopic and Optical Properties of the VO2+ ion doped TeO2-TiO2-ZnO-Nb2O5 glass system
Swapna,G. Upender,V. Sreenivasulu,M. Prasad 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.8
Studies such as optical absorption, Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, Electron paramagnetic resonance (EPR) spectroscopy and Differential scanning calorimetry (DSC) were carried out on VO2+ ion doped TeO2-TiO2-ZnO-Nb2O5 glass system. Raman and FTIR spectra of the glasses revealed the presence of [TeO3], [TeO4] and [NbO6] structural units in the glass network. The Urbach energy (E), cut-off wavelength (c), optical band gap (Eopt), optical basicity () and electron polarizability () of the glasses were determined from optical absorption studies. The density (), molar volume (Vm), oxygen molar volume (Vo) and refractive index (n) were also measured. Spin-Hamiltonian parameters were calculated from the EPR studies. When Nb2O5 was increased at the expense of ZnO, the density, optical band gap and Urbach energy of the glasses increased, and the electronic polarizability and optical basicity decreased. The EPR spectra clearly showed that vanadium was in the glass as VO2+ and occupied octahedral sites with tetrahedral compression. Spin-Hamiltonian parameters gk and g? decreased as Nb2O5 content increased in the glass. The glass transition temperature (Tg) also increased with increasing Nb2O5 content in the glass.
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G. Satheesh kumar,P. Sreenivasulu,M. Subhosh Chandra,Yong-Lark Choi,K. V. Mallaiah 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.3
In this study, the production of extracellular thermostable α-amylase by newly isolated thermophilic Alicyclobacillus acidocaldarius was detected on LB agar plates containing 1.0% soluble potato starch and incubated at 60oC. This extracellular α-amylase was purified to homogeneity by ammonium sulphate precipitation followed by Sephadex and ion-exchange chromatography. The α-amylase was purified to 8.138 fold homogeneity with a final recovery of 58% and a specific activity of 3,239 U/mg proteins. The purified α-amylase appeared as a single protein band on SDS-PAGE with a molecular mass of 94.5 kDa. Non-denaturing PAGE analysis showed one major band associated with enzyme activity, indicating the absence of isoenzymes. A TLC analysis showed maltose as major end product of the enzyme. The optimum assay temperature and pH for enzyme activity were 60oC and 6.0respectively; however, the enzyme activity was stable over a wide range of pH and temperatures. The α-amylase retained its activity in the presence of the denaturing agents - SDS, Triton X-100, Tween-20, Tween-80, and was significantly inhibited by EDTA and urea. Calcium ions increased the enzyme activity, while Hg2+, Zn2+, and Co2+had inhibitory effects. The Km and Vmax values were found to be 2.9 mg/mL and 7936 U/mL respectively.
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M. Padmavathi,P. Sreenivasulu,K. P. Srinivas,B. Ramesh,Ch. V. Subba Reddy,K. Navodayam,P. Ratan Babu,J. Krishnaprasadji 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.1
The genome of a potyvirus isolate associated with chlorotic spots and vein banding symptoms on Spathiphyllum spp., in Andhra Pradesh state, India was amplified by RT-PCR using degenerate potyvirus primers,amplicons cloned, and sequence (1.6 kb) analyzed. This virus isolate shared maximum identity of 74.8% and 80.2% at coat protein (CP) gene nucleotide (906 nucleotides)and amino acid (302 amino acids) levels,respectively with Dasheen mosaic virus (DsMV)-M13isolate reported from China. But its 3'-UTR (258nucleotides) had maximum identity of 62.5% with DsMV-Vietnam isolate. The deduced molecular weight of CP is 33.57 kDa and it contained DAG triplet in its Nterminal region. In CP amino acid based phylogenetic analysis, this virus isolate represented a separate branch but closer to DsMV isolates cluster. Based on the molecular criteria set for the discrimination of species and genus in the Potyviridae family, the present virus isolate was identified as a distinct virus species in the genus Potyvirus and proposed the name Spathiphyllum chlorotic vein banding virus (SCVbV).
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M. Venkata Ramanaa,N. Ramamanohar Reddy,G. Sreenivasulu,K.V. Siva kumar,B.S. Murty,V.R.K. Murthy 한국물리학회 2009 Current Applied Physics Vol.9 No.5
Multiferroic particulate composites of Ni0.83Co0.15Cu0.02Fe1.9O4-δ – NCCF and lead zirconate titanate (PZT) were prepared conventional ceramic method. The generic formulae x NCCF + (1-x) PZT where x = 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 mole fractions. The presence of two phases in multiferroic was confirmed with XRD technique. The dielectric constant and loss tangent were studied as a function of frequency (100 Hz to 1 M Hz) and temperature (30–500 ℃). The piezoelectric coefficient d33 were also studied on these particulate composites. The hysteresis behaviour was studied to understand the magnetic properties such as saturation magnetization (Ms) and magnetic moment (μB). The static magnetoelectric (ME) voltage coefficient was measured as a function of dc magnetic bias field. A high value of ME output (3151 mV/Oe.cm) was obtained in the composite containing 50% highly magnetostrictive ferrite component NCCF – 50% highly piezoelectric ferroelectric component PZT. These multiferroic particulate composites are used as phase shifters, magnetic sensors, cables etc. Multiferroic particulate composites of Ni0.83Co0.15Cu0.02Fe1.9O4-δ – NCCF and lead zirconate titanate (PZT) were prepared conventional ceramic method. The generic formulae x NCCF + (1-x) PZT where x = 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 mole fractions. The presence of two phases in multiferroic was confirmed with XRD technique. The dielectric constant and loss tangent were studied as a function of frequency (100 Hz to 1 M Hz) and temperature (30–500 ℃). The piezoelectric coefficient d33 were also studied on these particulate composites. The hysteresis behaviour was studied to understand the magnetic properties such as saturation magnetization (Ms) and magnetic moment (μB). The static magnetoelectric (ME) voltage coefficient was measured as a function of dc magnetic bias field. A high value of ME output (3151 mV/Oe.cm) was obtained in the composite containing 50% highly magnetostrictive ferrite component NCCF – 50% highly piezoelectric ferroelectric component PZT. These multiferroic particulate composites are used as phase shifters, magnetic sensors, cables etc.
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Padmavathi, M.,Srinivas, K.P.,Reddy, Ch. V. Subba,Ramesh, B.,Navodayam, K.,Krishnaprasadji, J.,Babu, P. Ratan,Sreenivasulu, P. The Korean Society of Plant Pathology 2011 Plant Pathology Journal Vol.27 No.1
The genome of a potyvirus isolate associated with chlorotic spots and vein banding symptoms on Spathiphyllum spp., in Andhra Pradesh state, India was amplified by RT-PCR using degenerate potyvirus primers, amplicons cloned, and sequence (1.6 kb) analyzed. This virus isolate shared maximum identity of 74.8% and 80.2% at coat protein (CP) gene nucleotide (906 nucleotides) and amino acid (302 amino acids) levels, respectively with Dasheen mosaic virus (DsMV)-M13 isolate reported from China. But its 3'-UTR (258 nucleotides) had maximum identity of 62.5% with DsMV-Vietnam isolate. The deduced molecular weight of CP is 33.57 kDa and it contained DAG triplet in its N-terminal region. In CP amino acid based phylogenetic analysis, this virus isolate represented a separate branch but closer to DsMV isolates cluster. Based on the molecular criteria set for the discrimination of species and genus in the Potyviridae family, the present virus isolate was identified as a distinct virus species in the genus Potyvirus and proposed the name Spathiphyllum chlorotic vein banding virus (SCVbV).
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G. Satheesh Kumar(사티시 쿠마르),M. Subhosh Chandra(수보쉬 찬드라),K. V. Mallaiah(브이 말라이아),P. Sreenivasulu(스리니바슐루),Yong Lark Choi(최용락) 한국생명과학회 2012 생명과학회지 Vol.22 No.4
고온성 α-아밀라제를 생산하는 내열성 Alicyclobacillus acidocaldarius 균을 인도 Tirupati, Andhra Pradesh 지역의 가열한 미강 열수 추출물에서 분리하였다. 분리균인 내열성 Alicyclobacillus acidocaldarius가 생산하는 세포 외 α-아밀라제의 생산과 성장에 미치는 배양조건을 실험실 규모로 조사하였다. 그 결과 α-amylase의 고생산 최적 조건은 온도 60oC, pH 6.0 및 배지의 전분농도 1.0%, yeast extract와 tryptone은 0.2%를 나타냈다. Surfactantslike Tween-20과 SDS 같은 계면활성제는 0.02%까지 균주의 성장과 효소 생산을 증가 시켰으나, 그 이상의 농도에서는 α-amylase 효소의 생산이 현저하게 감소하였다. A thermophilic Alicyclobacillus acidocaldarius, which produces thermostable α-amylase, was isolated from the hot water effluent of a boiled rice mill near Tirupati, Andhra Pradesh, India. The effect of different culture conditions on the growth and production of extracellular α-amylase by thermophilic A. acidocaldarius was investigated in laboratory scale. The results showed that the optimum conditions for the production of α-amylase are a temperature of 60°C, pH of 6.0, and medium starch concentration of 1.0%, and yeast extract and tryptone of 0.2%. Surfactants, like Tween-20 and SDS, up to 0.02%, were found to increase the bacterial growth and enzymes. Further increase in their concentration resulted in significantly decreased enzyme production.
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