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일본 Okinawa지역에서 두류유전자원의 수집과 Vigna riukiuensis의 지리적 변이
윤문섭,Kaga, Akito,Tomooka, Norihiko,Vaughan, Duncan,백형진,김창영 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4
일본 남서제도에 자생하는 두류 근연야생종집단의 분포와 집단구조를 파악하기 위해서 1999년 10월 21일부터 29일 까지 오끼나와 지역의 Hateruma, Tarama, Yonaguni 섬들에서 탐색ㆍ수집을 수향한 결과와 이들 섬으로부터 수집된 Vigna riukiuensis 집단에 대한 AFLP분석을 통하여 그들의 지리적 변이를 분석한 결과는 다음과 같다. 1. 일본의 본도에서는 발견되지 않는 Vigna riukiuensis, V. reflexo-pilosa, V. luteola, V. minima, Canavalia rosea 및 Glucine tabacina와 같은 야생종을 수집하였으며, 또한 재배종인 Macroptilium atropureum, Vigna unguiculate 및 Glycine max를 수집하였다. 2. V. riukiuensis는 잔디밭에서만 발견되었고, V. marina와 C. rosea는 해안가의 모래사장에서 주로 발견되어 두류의 내염성 육종에 유용한 재료가 될 것으로 기대되었다. 서식집단의 범위는 10여 개체에서부터 1㏊에 이르기까지 매우 다양했다. G. tabacina는 Miyako의 바닷가와 인접한 잔디밭에서만 발견되었으며, 사료작물로 도입된 M. atropureum은 대부분의 섬에서 반 재배형으로 널리 분포하고 있었다. 3. 수집된 자원은 총 74점으로서 V. riukiuensis 27점, V. reflexo-pilosa 10점, V. marina 17점, V. luteola 2점, V. unguiculate 5점, G. tabacina 5점, G. max 1점, M. atropureum 3점 및 C. rosea 4점을 각각 포함했다. 또한 이들 자원에 대한 근류균의 수집과 식물표본이 만들어 졌으며, 각 수집지점의 위치가 기록되었다. 4. V. riukiuensis집단들 사이의 지리적 유연관계는 principal coordinate analysis(PCA)에 의해 처음 두개의 주요성분은 전체변이의 57.9%를 설명했다. 전체 변이의 46.0%를 설명하는 PCA1은 Yonaguni-Ishigaki-Iromote-Hateruma집단과 Miyakowlqeks으로 구분시켰다. 전체변이의 11.9%를 설명하는 PCA2에 의해 Haterumawlqeks은 Yonaguni-Ishigaki-Iromote집단과 분리되었다. Exploration and collection of legume genetic resources was conducted on the southern islands of Okinawa prefecture, Hateruma, Iriomote, Irabu, Ishigaki, Miyako, Tarama and Yonaguni islands, Japean, from 21^(st) to 29^(th) October. 1999. From these islands, the following wild species were collected Vigna riukiuensis, V, reflexo-pilosa, V. luteola, V. marina, Canavalia rosea and Glycine tabacina and seeds or root nodules of the following cultivated species Macroptilium atropurpureum. Vigna unguicullata and Glycine max. The wild species collected were generally found in open herbaceous habitats such as roadside bushes, lawns or on beaches. Several species have specific adaptations such as V. riukiuensis and G. tabacina whic can survive in cut lawns. V. marina and C. rosea grow along sandy beaches of every island visited and may have physiological tolerances, such as salt tolerance, which may be of value in legume breeding. Population size varied greatly within and between species from less than 10 plants to covering more than 1ha. M. atropurpureum, introduced as a forage crop, has escaped and is widely changing. During this exploration and collection, V. riukiuensis and V. reflexo-pilosa , from the subgenus Ceratotropis were both found on Ishigaki, Iriomote, Yonaguni and Miyako islands. On the small island of Irabu and Tarama only V. reflexo-pilosa from the subgenus Ceratotropis was found. On Hateruma only V. riukiuensis from subgenus Ceratotropis was found. A total of 74 wild legume seed samples were collected. The comprehensive collection will enable diversity analysis to highlight islands and populations of particular genetic diversity for conservation and evaluation. Geographical variation of V. riukiuensis among the collected species was found within it's populations by AFLP analysis. Populations of Miyako island were genetically different from of other islands of Okinawa based on PCA.
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Saori Miyazaki,Koutatsu Maruyama,Kiyohide Tomooka,Shinji Nishioka,Noriko Miyoshi,Ryoichi Kawamura,Yasunori Takata,Haruhiko Osawa,Takeshi Tanigawa,Isao Saito 대한골다공증학회 2023 Osteoporosis and Sarcopenia Vol.9 No.3
Objectives: Few studies examined the association between deterioration of masticatory ability assessed by objective marker and physical function. Therefore, we examined the association between salivary flow rate which is one of the objective and surrogate marker of masticatory ability and lower Timed Up & Go (TUG) performance which is one of major measurement of physical function among aging Japanese. Methods: This cross-sectional study enrolled 464 Japanese aged 60e84 years old. Participants chewed tasteless and odorless gum for 5 min, calculated stimulated salivary flow rate (g/min) during all chews. The 3 m TUG was conducted, and 75th percentile value (6.8 s for men and 7.0 s for women) or higher was defined as lower TUG performance. Logistic regression analysis was used to examine the association between stimulated salivary flow rate and lower TUG performance. Results: We found that the stimulated salivary flow rate tended to be negatively associated with the TUG time. We also observed significant negative association between stimulated salivary flow rate and lower TUG performance; the multivariable-adjusted OR (95% confidence interval, CIs) of lower TUG performance for the highest quartile of stimulated salivary flow rate compared with the lowest quartile was 0.34 (0.16-0.69, P for trend = 0.02). Further adjusting for BMI, the association was attenuated but remaind significant; the OR (95% CIs) in highest quartile was 0.37 (0.18-0.76, P for trend = 0.04). Conclusions: Higher stimulated salivary flow, which means well masticatory ability, was inversely associated with lower TUG performance in the aging Japanese population.
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