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RISS 인기검색어
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- 저자 : Tie-ying Li (검색결과 6 건)
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Li, Tie-Fu,Deng, Ying-Jie,Ma, Guang-Li,Jin, Jie,Li, Song Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.11
This paper describes an inductively coupled plasma emission spectrometric method for the analysis of Fe in rat plasma. Calibration curves were obtained in the range of 0.125-1.50 ${\mu}g{\cdot}mL^{-1}$. The relative standard deviation ranges from 5.93% to 6.80%, and accuracy was between 87.6 and 102.0%. Dilution with water had no influence on the performance of the method, which could then be used to quantify Fe concentration in plasma up to 0.50 ${\mu}g{\cdot}mL^{-1}$. The limit of quantification was 0.10 ${\mu}g{\cdot}mL^{-1}$. At this level, the average relative standard deviation was 6.8%. The results indicate that the method meets the accuracy and precision requirements for the pharmacokinetic studies. The Fe concentration in rat plasma was measured and the main pharmacokinetic parameters were calculated by Topfit 2.0 (GmbH. Shering AG, Godecke AG, Germany).
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( Yin Tie ),( Li Li Miao ),( Fei Fei Guan ),( Gui Li Wang ),( Qing Peng ),( Bing Xue Li ),( Guo Hua Guan ),( Ying Li ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
An extremely thermostable xylanase gene, xynB, from the hyperthermophilic bacterium Thermotoga maritima MSB8 was successful expressed in Kluyveromyces lactis. The response surface methodology (RSM) was also applied to optimize the medium components for the production of XynB secreted by the recombinant K. lactis. The secretion level (102 mg/l) and enzyme activity (49 U/ml) of XynB in the optimized medium (yeast extract, lactose, and urea; YLU) were much higher than those (56 mg/l, 16 U/ml) in the original medium (yeast extract, lactose, and peptone; YLP). The secretory efficiency of mature XynB was also improved when using the YLU medium. When the mRNA levels of 13 characterized secretion-related genes in the K. lactis cultured in YLP and YLU were detected using a semiquantitative RT-PCR method, the unfolded protein response (UPR)-related genes, including ero1, hac1, and kar2, were found to be up-regulated in the K. lactis cultured in YLU. Therefore, the nutrient ingredients, especially the nitrogen source, were shown to have a significant influence on the XynB secretory efficiency of the host K. lactis.
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Qin, Sheng-Li,Schilke, Peter,Wu, Jingwen,Wu, Yuefang,Liu, Tie,Liu, Ying,Sá,nchez-Monge, Á,lvaro IOP Publishing 2015 The Astrophysical journal Vol.803 No.1
<P>We report on the Submillimeter Array (SMA) observations of molecular lines at 270 GHz toward the W3(OH) and W3(H2O) complex. Although previous observations already resolved the W3(H2O) into two or three subcomponents, the physical and chemical properties of the two sources are not well constrained. Our SMA observations clearly resolved the W3(OH) and W3(H2O) continuum cores. Taking advantage of the line fitting tool XCLASS, we identified and modeled a rich molecular spectrum in this complex, including multiple CH3CN and CH3OH transitions in both cores. HDO, C2H5CN, (OCS)-C-13, and vibrationally excited lines of HCN, CH3CN, and CH3OCHO were only detected in W3(H2O). We calculate gas temperatures and column densities for both cores. The results show that W3(H2O) has higher gas temperatures and larger column densities than W3(OH) as previously observed, suggesting physical and chemical differences between the two cores. We compare the molecular abundances in W3(H2O) to those in the Sgr B2(N) hot core, the Orion KL hot core, and the Orion Compact Ridge, and discuss the chemical origin of specific species. An east-west velocity gradient is seen in W3 (H2O), and the extension is consistent with the bipolar outflow orientation traced by water masers and radio jets. A north-south velocity gradient across W3(OH) is also observed. However, with current observations we cannot be assured whether the velocity gradients are caused by rotation, outflow, or radial velocity differences of the subcomponents of W3(OH).</P>
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Lateral Structural Performance of Column Frame Layer and Dou-Gong Layer in a Timber Structure
Xian-jie Meng,Tie-ying Li,Qing-shan Yang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.2
In this study, a 1:2 scale model of a timber building with a column frame layer and a Dou-Gong layer was fabricated according to the construction method that prevailed during the Song dynasty, China (A.D. 960–1279). Three quasi-static tests were conducted on the model under three levels of vertical loads to study the seismic response of the column frame layer and Dou-Gong layer in a timber structure. The experimental results indicated that the ultimate loading state of the timber structure was mainly controlled by the lateral displacement of the column frame layer. The hysteretic behaviour and stiffness characteristics of the column frame layer were quite similar to those of the overall structure. The stiffness of the Dou-Gong layer was much higher than that of the column frame layer, and no stiffness degradation was observed in the Dou-Gong layer. Fairly little energy was dissipated by the Dou-Gong layer; more than 80% of the structural energy dissipation that occurred was attributable to the column frame layer. Moreover, based on the deformation features of the column frame and Dou-Gong layer, a theoretical model for estimating the restoring force of the overall structure was established.
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Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma
Tang, Jin-Feng,Yu, Zhong-Hua,Liu, Tie,Lin, Zi-Ying,Wang, Ya-Hong,Yang, La-Wei,He, Hui-Juan,Cao, Jun,Huang, Hai-Li,Liu, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.
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