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Correlations between abnormalities of morphological details and DNA fragmentation in human sperm
Nguyen, Hiep Tuyet Thi,Dang, Hong Nhan Thi,Nguyen, Thai Thanh Thi,Nguyen, Trung Van,Dang, Thuan Cong,Nguyen, Quoc Huy Vu,Le, Minh Tam The Korean Society for Reproductive Medicine 2022 Clinical and Experimental Reproductive Medicine Vol.49 No.1
Objective: As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.
Anti-inflammatory tirucallane saponins from Paramignya scandens.
Phan, Nguyen Huu Toan,Thuan, Nguyen Thi Dieu,Ngoc, Ninh Thi,Thao, Nguyen Phuong,Kim, Sohyun,Koh, Young Sang,Thanh, Nguyen Van,Cuong, Nguyen Xuan,Nam, Nguyen Hoai,Kiem, Phan Van,Kim, Young Ho,Minh, Cha Pharmaceutical Society of Japan 2015 Chemical & pharmaceutical bulletin Vol.63 No.7
<P>Five new tirucallane saponins, paramignyosides A-E (1-5), were isolated from the water fraction of the Paramignya scandens stem and leaves. Their structures were elucidated on the basis of spectroscopic evidence including high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and one dimensional (1D)- and 2D-NMR. The effects of isolated compounds on pro-inflammatory cytokines were evaluated by measuring the production of interleukin (IL)-12 p40, IL-6, and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs). Paramignyoside C (3) exhibited selective and potent inhibitory effect (IC50=5.030.19??M) on the production of IL-12 p40 comparable to that of the positive control, SB203580 (IC50=5.000.16??M). Further studies are required to confirm efficacy in vivo and the mechanism of anti-inflammatory effects.</P>
Loc My Thi Nguyen,Tien-Trung Nguyen,Thanh Thi Nghiem,Hien Thu Thi Le,Thao Phuong Thi Trinh,Thuan Van Pham,Thanh Chi Nguyen,Linh Khanh Hoang,Trung Tran 한국과학학술지편집인협의회 2020 Science Editing Vol.7 No.1
In the context of the need to ensure appropriate signalling of the publication of high-quality, international-calibre publications in Vietnam, as well as new policies to improve the quality and effectiveness of scientific research in Vietnam, it is practical to investigate the possibility of developing a national open access database (NOAD). This study aims to answer the question of whether it is necessary to establish a NOAD in Vietnam. We used document analysis to evaluate issues related to NOADs. The results of this study show the complexity, lack of consistency, and difficulty in obtaining practical statistics and assessing research and scientific records in Vietnam today. Furthermore, the findings of this study imply that it is necessary to establish a NOAD of scientific research in Vietnam. The information in this report can be used to develop a NOAD for Vietnam in particular, and for any country that lacks one in general.
Marital Status and Satisfaction of Online Shoppers in the Beauty and Cosmetic Sector in Vietnam
NGUYEN, Thuan Thi Nhu,HOMOLKA, Lubor Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.2
We investigate the impact of marital status on the levels of satisfaction of online shoppers in the beauty and cosmetics industry in Vietnam. We find a significant difference in satisfaction between married and divorced/separated online shoppers. More specifically, the latter reveals higher level of satisfaction than the former. Extended analyses further show that this finding is only observed for female online shoppers whilst it is insignificant for their male counterparts. Moreover, we find significant differences in determinants of customer satisfaction between three different groups of online shoppers: single, married, and divorced/separated. While married individuals' satisfaction is affected by all factors (Online shopping experience; Seller Services; External Incentives; Security/Privacy), that of their divorced/separated peers are influenced by only seller services and external incentives. Also, single participants are satisfied with their online shopping driven by their online shopping experience and external incentives. Our findings contribute to the stream of customer satisfaction literature, and to the studies in beauty and cosmetics sector as well as online shopping trends in Vietnam. They contain implications for existing online businesses and new or potential market entrants as to which customer demographic factors have significant influences in terms of customer psychology, behaviour and their satisfaction.
Gender Differences in Determinants of Customer Satisfaction in Beauty and Cosmetic E-commerce
NGUYEN, Thuan Thi Nhu Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.10
This study investigates the gender differences in determinants of customers' satisfaction when purchasing online beauty and cosmetics products in Vietnamese market. To do so, we construct a data set via online survey of 419 Vietnamese customers including both males and females, and examine such sample using t-tests and multiple regressions. Our regression model is constructed based on our five-construct model including online shopping experience, customer service, external incentives, security and privacy, and personal characteristics. The t-tests results reveal that the mean difference of customer satisfaction (negative coefficient) between the two groups of customers (females and males) is statistically significant. This suggests that the purchasing satisfaction of male customers is significantly higher than that of their female counterparts. Similarly, we also find that male customers are more satisfied with their online shopping experience, customer service and external incentives offered by the online sellers than female peers. Furthermore, regression results for full sample show that, on average, the online shopping experience, customer service and external incentives are significantly and positively related to customer satisfaction. Yet, such positive effect of customer service on customer satisfaction is more likely for males while females' satisfaction is more positively influenced by security and privacy.
Hoang Thi Le,나민균,Do Thi Ha,Chau Thi Anh Minh,Tae Hoon Kim,Phan Van Kiem,Nguyen Duy Thuan 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.1
Phytochemical investigation of the stem barks of Canarium bengalense (Burseraceace) resulted in the isolation of a new flavone glycoside (5) together with six known compounds (1-4, 6, and 7). The chemical structure of the new compound was elucidated as 3'-hydroxy-7,4'-dimethoxyflavone-5-O-α-L-arabinofuranosyl-(1→6)-β-D-glucopyranoside by means of 1D and 2D NMR (1H-1H COSY, HMQC, and HMBC) and MS analyses. To evaluate the in vitro cytoprotective effect, the isolates (1-7) were tested against hydrogen peroxide (H2O2)-induced damage in primary cultured hepatocytes. The viability of hepatocytes was increased by treatment with each compound, except compound 1. Compounds 3, 4, and 7 exerted cytoprotective effects comparable to curcumin, the positive control. Our results suggest that the cytoprotective constituents of C. bengalense may contribute to its traditional use in the treatment of tumor and liver damage.
A Phosphate Starvation-Inducible Ribonuclease of Bacillus licheniformis
( Thanh Trung Nguyen ),( Minh Hung Nguyen ),( Huy Thuan Nguyen ),( Hoang Anh Nguyen ),( Thi Hoi Le ),( Thomas Schweder ),( Britta Jurgen ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.8
The BLi03719 protein of Bacillus licheniformis DSM13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. In this study, the function of this phosphate starvation inducible protein was determined. An amino-acid sequence analysis of the BLi03719-encoding gene showed a high similarity with genes encoding the barnase of Bacillus amyloliquefaciens FZB42 and binase-like RNase of Bacillus pumilus SARF-032. The comparison of the control strain and a BLi03719-deficient strain revealed a strongly reduced extracellular ribonuclease activity of the mutant. Furthermore, this knockout mutant exhibited delayed growth with yeast RNA as an alternative phosphate and carbon source. These results suggest that BLi03719 is an extracellular ribonuclease expressed in B. licheniformis under phosphate starvation conditions. Finally, a BLi03719 mutant showed an advantageous effect on the overexpression of the heterologous amyE gene under phosphate-limited growth conditions.
Dinh Bang Nguyen,Thanh Phuong Dao,Hung Thuan Tran,Van Noi Nguyen,안대희,Thi Dieu Cam Nguyen 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.5
The aim of this study is to evaluate phenol degradation capability of silver modified titanium dioxide nanomaterial on bentonite support (Ag-TiO2/Bent). The material was synthesized as photocatalyst by adding Ag-TiO2 sol into bentonite suspension. The experimental results revealed that photooxidation activity of Ag-TiO2/Bent was greatly higher than that of Ag-TiO2 and TiO2/Bent. The phenol removal efficiency was 23.25%, 35.41% and 98.94% for Ag-TiO2, TiO2/Bent and Ag-TiO2/Bent, respectively. The dispersion of silver modified TiO2 on bentonite support significantly enhances photocatalytic activity under solar radiation due to surface plasmon resonance formation and prevention of anatase-to-rutile phase transformation.
Van Noi Nguyen,Thi Dieu Cam Nguyen,Thanh Phuong Dao,Hung Thuan Tran,Dinh Bang Nguyen,안대희 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2
Organoclays were synthesized by exchanging inorganic cations between layers in Thanh Hoa bentonite using organic cations including benzylhexadecyldimethylammonium (BHDDM+), dimethyldioctadecylammonium (DMDOD+) and benzylstearyldimethylammonium (BSDM+). Inserting organic cations increases material interlayer distance significantly (from 15 A˚ to 40 A˚ ) and simultaneously enhances affinity of materials toward organic pollutants. The results show that adsorption capacity of organics on organoclays strongly depends on affinity between organic substances and ammonium cations rather than on interlayer distance of organoclays. This means that the sorption of organoclays for organic contaminants was significantly influenced by the nature of the surfactants added to the clay.
Thu Ha Nguyen,Thi Lan Pham,Anh Quan Cao,Tuan Anh Nguyen,Xuan Minh Vu,Thi My Hanh Le,Van Thuan Le,Seiichi Kawahara,Dai Lam Tran 한국고분자학회 2024 Macromolecular Research Vol.32 No.4
This study describes the preparation and characterization of a green and safe membrane based on a natural polymer for metal adsorption. Natural rubber-grafted-(2-hydroxyethyl methacrylate) with a special nanostructure was synthesized by graft copolymerization of 2-hydroxyethyl methacrylate (HEMA) onto the surface of natural rubber (NR) particles using tert-butylhydroperoxide/tetraethylenepentamine as initiators. Optimal conditions for achieving high conversion and grafting efficiency were identified. Characterization of the as-synthesized samples was performed using Fourier-transform infrared spectroscopy, transmission electron microscopy, thermogravimetric analysis, tensile measurement, swelling degree determination, and cytotoxicity testing. The results revealed that HEMA formed a nanoscale matrix surrounding NR particles, which improved the tensile strength, thermal resistance, and swelling degree of the as-prepared samples. Cytotoxicity testing demonstrated that the membrane was safe for human use, as it did not exhibit toxicity to Vero cells at concentrations up to 1024 μg/mL. Furthermore, the membrane displayed a high adsorption capacity toward Fe3+ and was well described by Koble-Corrigan isotherm model and the first–second-order kinetic equation. Moreover, the membrane demonstrated excellent recyclability maintaining its adsorption ability towards Fe3+ ions over five consecutive cycles. Overall, these findings may recommend the NR-HEMA membrane as a promising candidate for metal removal applications.