Effect of Aminoglycoside Antibiotics on in-Vitro Morphogenesis from Cultured Cells of Chrysanthemum and Tobacco

Teixeira da Silva, Jaime A.,Fukai, Seiichi The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.1

Successful genetic transformation of plants requires non-chimeric selection of transformed tissues and their subsequent regeneration. With rare exceptions, most transformation protocols still rely heavily on antibiotics for selecting transgenic cells that contain an antibiotic-degrading selectable marker gene. Here, the morphogenic capacity of in-vitro explants of chrysanthemnum and tobacco stems and leaves (control and transgenic) changed with the addition of aminoglycoside antibiotics (AAs), In a test of 6 AAs, phytotoxicity occurred at concentrations of 10 to 25 and 50 to 100$\mu\textrm{g}$ $mL^{-1}$ in chrysanthemum and tobacco explants, respectively. Light conditions as well as explant source and size also had significant effects. The use of transverse thin cell layers (tTCLs), in conjunction with high initial AA selection levels, supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow-cytometric analyses revealed no endodu-plication in chrysanthemum, even at high AA levels. However, this phenomenon was observed in tobacco calli(8C or more), even at low AA concentrations (i.e., 5 to 10 $\mu\textrm{g}$ mL$^{-1}$ ).

Effect of Aminoglycoside Antibiotics on in-Vitro Morphogenesis from Cultured Cells of Chrysanthemum and Tobacco

Silva, Jaime A. Teixeira da,Fukai, Seiichi 한국식물학회 2003 Journal of Plant Biology Vol.46 No.2

Successful genetic transformation of plants requires non-chimeric selection of transformed tissues and their subsequent regeneration. With rare exceptions, most transformation protocols still rely heavily on antibiotics for selecting transgenic cells that contain an antibiotic-degrading selectable marker gene. Here, the morphogenic capacity of in-vitro explants of chrysanthemum and tobacco stems and leaves (control and transgenic) changed with the addition of aminoglycoside antibiotics (AAs). In a test of 6 Ah, phytotoxicity occurred at concentrations of 10 to 25 and 50 to 100 ㎍mL^(-1) in chrysanthemum and tobacco explants, respectively. light conditions as well as explant source and size also had significant effects, The use of transverse thin cell layers (tTCLs), in conjunction with high initial AA selection levels, supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow-cytometricanalyses revealed no endoduplication in chrysanthemum, even at high AA levels. However, this phenomenon was observed in tobacco calli (8C or more), even at low AA concentrations (i.e., 5 to 10 ㎍mL^(-1).

Tissue Culture and Micropropagation of Tree Peony (Paeonia suffruticosa Andr.)

da Silva, Jaime A. Teixeira,Shen, Miaomiao,Yu, XiaoNan 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3

Tree peony (Paeonia suffruticosa) is one of China's most traditional flowers. It is also one of the most remarkable flowering ornamentals with a rich history of breeding. The speed and success of propagation have always been a major concern for breeders and cultivators. Tissue culture is one of the few effective methods to overcome these inherent difficulties. This article reviews advances made on various aspects of tree peony tissue culture and micropropagation, including the in vitro culture of buds, stem apex, leaves and petioles, sexual embryos and ovules, somatic embryos, anther and callus. Since the vast majority of literature is published in Chinese, this review provides a unique and valuable resource and spring-board from which to initiate or further studies related to in vitro culture of tree peony.

Evaluation of salt and root-zone temperature stresses in leafy vegetables using hydroponics as a clean production cultivation technique in northeastern Brazil

da Silva Mairton Gomes,Gheyi Hans Raj,da Silva Lucas Lesqueves,de Souza Thaisa Teixeira,Silva Petterson Costa Conceição,Queiroz Laila de Andrade,dos Santos Thainan Sipriano,Soares Tales Miler 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

Hydroponics has represented an emerging solution for regions with freshwater scarcity. Besides requiring a lower volume of water for production, this cultivation technique allows the use of brackish waters with high salinity levels. Under normal cultivation conditions (i.e., without any environmental control), plants are exposed to other abiotic stresses, such as tem perature, which can further enhance the salinity eff ect. Given the above, three experiments were conducted using a nutrient f i lm technique (NFT) hydroponic system: from April to May (autumn) with endive ( Cichorium endivia L., cv. Gigante Barbarella), from May to July (autumn/winter) with lettuce ( Lactuca sativa L., cv. Rubinela and cv. Veneranda), and from August to October (winter/spring) with chicory ( Cichorium intybus L., cv. Pão de Açúcar and cv. Radiche Todo Ano), in 2021. The trials aimed to evaluate the isolated and/or combined eff ect of salt and root-zone temperature stresses on yield, water use effi ciency, and visual quality of these vegetables. In all experiments, plants were subjected to stresses for 25 days in a randomized block design with a 2 × 4 factorial arrangement: two levels of electrical conductivity of water –ECw (0.30 and 5.50 dS m –1 for endive and chicory; 0.25 and 6.50 dS m −1 for lettuce) combined with four root-zone temperatures – RZTs (ambient-ARZT, ARZT + 2 °C, ARZT + 4 °C, and ARZT + 6 °C, corresponding to the mean temperatures of 24.72, 26.90, 28.77, and 30.73 °C for endive; 24.20, 26.00, 28.00, and 29.99 °C for lettuce; 27.12, 28.93, 31.01, and 32.86 °C for chicory). The isolated eff ects of RZT stresses or ECw levels on endive traits were observed, with leaf fresh matter losses of approxi mately 17 and 44% at higher ARZT + 6 °C (mean of 30.73 °C) and salinity (ECw 5.50 dS m –1 ), respectively. Shoot fresh matter losses of around 32 and 52% (Veneranda and Rubinela lettuces, respectively) and 65% (Radiche Todo Ano chicory) occurred only due to salt stress. The interaction aff ected the shoot fresh matter of Pão de Açúcar chicory, with approximate losses of 80% at higher ARZT + 6 °C (mean of 32.86 °C) and salt stress (ECw 5.50 dS m –1 ) than ARZT and ARZT + 2 °C and without salt stress (ECw 0.30 dS m –1 ). Despite these reductions, visual quality of plants was not depreciated. Therefore, the present study suggested cultivating leafy vegetables such as lettuce, chicory, and endive using brackish waters, even under high temperatures of nutrient solutions.

Micropropagation of Sweetpotato (Ipomoea batatas) in a novel $CO_2$-Enriched Vessel

Silva Jaime A. Teixeira da,Giang Dam Thi Thanh,Tanaka Michio The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.1

To overcome various disadvantages of conventional cul-ture vessels for micropropagation, a novel disposable vessel, the 'Vitron', made of a multi-layered $OTP^{(R)}$ film and supported by a polypropylene frame, was developed. The film possesses superior properties such as: high light transmittance, low water vapor transmittance and thermal stability and in particular, high gas-permeability. Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in Vitron and polycarbonate vessels on $3\%$ sugar-containing agar on MS medium and placed at 3000 ppm $CO_2$-enrichment at a low photosynthetic photon flux density (PPFD) ($45{\mu}mol\;m^{-2}\;s^{-1}$). The in vitro and ex vitro growth, and the net photosynthetic rate of in vitro and ex vitro plantlets were significantly enhanced in the Vitron compared to those cultured in a polycarbonate vessel. Explants that were cultured on the same MS medium under low PPFD at various $CO_2$ concentrations were also cultured at 3000 ppm $CO_2$- enrichment at various PPFD: 30, 45, 60, 75 and $90{\mu}mol\;m^{-2}\;s^{-1}$. The best in vitro and ex vitro growth obtained for 3000 ppm $CO_2$-enrichment at $75{\mu}mol\;m^{-2}\;s^{-1}$ PPFD. The novel Vitron vessel, when placed under the two conditions, may replace conventional culture vessels for the successful micropropagation of sweetpotato.

Allelopathic Potential of Select Gymnospermous Trees

da Silva, Jaime A. Teixeira,Karimi, Javad,Mohsenzadeh, Sasan,Dobranszki, Judit Institute of Forest Science 2015 Journal of Forest Science Vol.31 No.2

Allelopathy is an ecological phenomenon that refers to the beneficial or harmful effects of one plant on another plant, both crop and weed species, by the release of organic chemicals (allelochemicals) from plant parts by leaching, root exudation, volatilization, residue decomposition in soil and other processes in both natural and agricultural systems. Allelopathy can affect many aspects of plant ecology including occurrence, growth, plant succession, the structure of plant communities, survival, dominance, diversity, and plant productivity. In this review, we describe the concept of allelopathy, some mechanisms of operation within plants and then focus on a select number of gymnospermous tree genera: Ephedra, Pinus, Taxus, Cedrus, Juniperus, Picea, Cunninghamia and Araucaria. Pinus, Taxus (yew) and Cedrus (cedar) trees have a strong negative allelopathic effect on the germination, growth, or development of other plant species in the forest community.

Tissue Culture and Micropropagation of Tree Peony (Paeonia suffruticosa Andr.)

Jaime A Teixeira da Silva,Miaomiao Shen,XiaoNan Yu 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3

Tree peony (Paeonia suffruticosa) is one of China’s most traditional flowers. It is also one of the most remarkable flowering ornamentals with a rich history of breeding. The speed and success of propagation have always been a major concern for breeders and cultivators. Tissue culture is one of the few effective methods to overcome these inherent difficulties. This article reviews advances made on various aspects of tree peony tissue culture and micropropagation, including the in vitro culture of buds, stem apex, leaves and petioles, sexual embryos and ovules, somatic embryos, anther and callus. Since the vast majority of literature is published in Chinese, this review provides a unique and valuable resource and spring-board from which to initiate or further studies related to in vitro culture of tree peony.

A call for greater editorial responsibilities

Jaime A. Teixeira da Silva 한국과학학술지편집인협의회 2015 Science Editing Vol.2 No.2

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Mechanical Stress Modifies Endogenous Ethylene and Gibberellin Production in Chrysanthemum Plants

Jaime A. Teixeira da Silva,Tetsuo Hara,Shengze Jiang,Fei Zhao,Chengshu Zheng 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Does Cloned Template Text Compromise the Information Integrity of a Paper, and is it a New Form of Text Plagiarism?

Jaime A. Teixeira da Silva 건국대학교 GLOCAL(글로컬)캠퍼스 지식콘텐츠연구소 2023 International Journal of Knowledge Content Develop Vol.13 No.2

Word templates exist for select journals, and their primary objective is to facilitate submissions to those journals, thereby optimizing editors’ and publishers’ time and resources by ensuring that the desired style (e.g., of sections, references, etc.) is followed. However, if multiple unrelated authors use the exact same template, a risk exists that some text might be erroneously cloned if template-based papers are not carefully screened by authors, journal editors or proof copyeditors. Elsevier Procedia® was used as an example. Select cloned text, presumably derived from MS Word templates used for submissions to Elsevier Procedia® journals, was assessed using Science Direct. Typically, in academic publishing, identical text is screened using text similarity software during the submission process, and if detected, may be flagged as plagiarism. After searching for “heading should be left justified, bold, with the first letter capitalized”, 44 Elsevier Procedia® papers were found to be positive for vestigial template text. The integrity of the information in these papers has been compromised, so these errors should be corrected with an erratum, or in the case of extensive errors and vast tracts (e.g., pages long) of template text, papers should be retracted and republished.