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RISS 인기검색어
- 검색키워드
- 저자 : Tariq Rafique (검색결과 3 건)
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Rehman Aziz Ur,Javed Muhammad Tariq,Ahmed Ishtiaq,Saeed Muhammad Adnan,Ehtisham-ul-Haque Syed,Rafique Muhammad Kamran,Sikandar Arbab,Nasir Amar,Ahmad Latif,Kashif Muhammad,Zeeshan Muhammad Abid 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.9
Objective: This study aimed to investigate the prevalence of paratuberculosis in cattle and buffaloes at twelve public dairy farms in Punjab, Pakistan.Methods: A total of 2,181 more than two-year-old animals (1,242 cattle and 939 buffaloes) were tested by avian tuberculin, i.e., killed purified protein derivative of <i>Mycobacterium avium</i> paratuberculosis and indirect enzyme linked immunosorbent assay (ELISA). Blood and fecal samples were collected from tuberculin positive animals. These samples were further processed by indirect ELISA. The data were analyzed using frequency analysis and logistic analysis procedures.Results: The prevalence of paratuberculosis at public dairy farms was 3.8%, as determined by tuberculin+ELISA test. It varied from 0.71% to 13.5% with a 100% herd prevalence. Multivariate logistic regression analysis revealed that species, milk production, total animals, total small ruminants, and total buffaloes were significantly associated with the occurrence of paratuberculosis. Odd ratio analysis revealed that with a one-kilogram increase in body weight, there will be a 0.006% increase in disease occurrence. With the increase in one animal in small ruminants and buffaloes, there will be 0.008% and 0.42% greater chances of developing paratuberculosis, respectively. Bivariate logistic regression analysis of cattle and buffaloes revealed that farm number, age, and total number of cattle were significantly associated with the occurrence of paratuberculosis. A one-month increase in lactation length increases the chance of tuberculosis by 0.004%; similarly, a one-liter increase in milk production increases the chance of disease by 10%. With each additional buffalo in the herd, there will be a 0.007% greater chance for the occurrence of paratuberculosis.Conclusion: This study concluded that tuberculin testing can be used in conjunction with ELISA to screen animals for paratuberculosis in countries with scarce resources, such as Pakistan. The efficacy of disease diagnosis can be improved by combining multiple tests. Objective: This study aimed to investigate the prevalence of paratuberculosis in cattle and buffaloes at twelve public dairy farms in Punjab, Pakistan. Methods: A total of 2,181 more than two-year-old animals (1,242 cattle and 939 buffaloes) were tested by avian tuberculin, i.e., killed purified protein derivative of Mycobacterium avium paratuberculosis and indirect enzyme linked immunosorbent assay (ELISA). Blood and fecal samples were collected from tuberculin positive animals. These samples were further processed by indirect ELISA. The data were analyzed using frequency analysis and logistic analysis procedures. Results: The prevalence of paratuberculosis at public dairy farms was 3.8%, as determined by tuberculin+ELISA test. It varied from 0.71% to 13.5% with a 100% herd prevalence. Multivariate logistic regression analysis revealed that species, milk production, total animals, total small ruminants, and total buffaloes were significantly associated with the occurrence of paratuberculosis. Odd ratio analysis revealed that with a one-kilogram increase in body weight, there will be a 0.006% increase in disease occurrence. With the increase in one animal in small ruminants and buffaloes, there will be 0.008% and 0.42% greater chances of developing paratuberculosis, respectively. Bivariate logistic regression analysis of cattle and buffaloes revealed that farm number, age, and total number of cattle were significantly associated with the occurrence of paratuberculosis. A one-month increase in lactation length increases the chance of tuberculosis by 0.004%; similarly, a one-liter increase in milk production increases the chance of disease by 10%. With each additional buffalo in the herd, there will be a 0.007% greater chance for the occurrence of paratuberculosis. Conclusion: This study concluded that tuberculin testing can be used in conjunction with ELISA to screen animals for paratuberculosis in countries with scarce resources, such as Pakistan. The efficacy of disease diagnosis can be improved by combining multiple tests.
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Khalid, Nauman,Ahmed, Iftikhar,Latif, Malik Shah Zaman,Rafique, Tariq,Fawad, Sardar Atiq The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Allium species are considered to be one of the world's oldest cultivated vegetables. Most commonly used species of garlic in Pakistan and India is Allium sativum, while Allium tuberosum is mainly consumed and cultivated in China, Southeast Asia, and North-east part of India. The present study was conducted to compare the antimicrobial activity, nutritional value and antioxidant profile of Allium sativum and Allium tuberosum. The outcome indicates that Allium tuberosum have slightly higher antimicrobial activity, higher mineral profile, and enriched in antioxidants in comparison with Allium sativum. The highest antimicrobial activity of Allium tuberosum was noticed against Staphylococcus aureus and Bacillus subtilis with 43.9 and 40.7 mm zone of inhibition using 100% extract. Allium tuberosum contains high contents of calcium ($28.662{\pm}.00mg/100g$), potassium ($10.62{\pm}0.50$) and zinc ($59.00{\pm}1.00$). Allium tuberosum also showed higher antioxidant activity ($0.24{\pm}0.03mg$ vitamin C equivalent (VCE)/g fresh weight in ferric reducing antioxidant power assay, $0.18{\pm}0.02mg\;VCE/g$ fresh weight in 2,2-diphenyl-1-picrylhydrazyl assay and $1.09{\pm}0.12mg\;VCE/g$ fresh weight in 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assay) in comparison with Allium sativum.
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Nauman Khalid,Iftikhar Ahmed,Malik Shah Zaman Latif,Tariq Rafique,Sardar Atiq Fawad 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Allium species are considered to be one of the world’soldest cultivated vegetables. Most commonly used species ofgarlic in Pakistan and India is Allium sativum, while Alliumtuberosum is mainly consumed and cultivated in China, SoutheastAsia, and North-east part of India. The present study wasconducted to compare the antimicrobial activity, nutritional valueand antioxidant profile of Allium sativum and Allium tuberosum. The outcome indicates that Allium tuberosum have slightly higherantimicrobial activity, higher mineral profile, and enriched inantioxidants in comparison with Allium sativum. The highestantimicrobial activity of Allium tuberosum was noticed againstStaphylococcus aureus and Bacillus subtilis with 43.9 and 40.7mm zone of inhibition using 100% extract. Allium tuberosumcontains high contents of calcium (28.662±.00mg/100 g), potassium(10.62±0.50) and zinc (59.00±1.00). Allium tuberosum alsoshowed higher antioxidant activity (0.24±0.03 mg vitamin Cequivalent (VCE)/g fresh weight in ferric reducing antioxidantpower assay, 0.18±0.02 mg VCE/g fresh weight in 2,2-diphenyl-1-picrylhydrazyl assay and 1.09±0.12 mg VCE/g fresh weight in2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assay) incomparison with Allium sativum.
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