Study on Flaking Resistance of Hot-dip Galvanizing Coating

( Tai Xiong Guo ),( Ping Yuan ),( Yong Qing Jin ),( Chun Fu Liu ),( Wei Li ) 한국부식방식학회 2010 Corrosion Science and Technology Vol.9 No.4

Immunomodulation in female B6C3F1 mice following treatment with Chai-Ling-Tang, a formula consisting of twelve herbs

Guo, Tai L.,Chi, Rui P.,Fu, Jonathan,White, Kimber L. Jr. 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.2

Chai-Ling-Tang (CLT) is a traditional Chinese herbal decoction consisting of twelve different herbs, and it has been used to treat a number of immune related disorders. However, the mechanism for its clinical effectiveness was not fully understood. In this study, CLT's effect on the immune system of female B6C3F1 mice was evaluated by examining the IgM antibody forming cell (AFC) response; natural killer (NK) cell activity; the expression of splenocyte surface markers; concanavalin (Con) A and anti-CD3 antibody-mediated T-lymphocyte proliferation; lipopolysaccharide-mediated-B cell proliferation, and the host resistances to the $Listeria$ $monocytogenes$ bacterial infection and B16F10 melanoma. The results from these studies suggest that the administration of CLT at doses between 400 and 800 mg/kg/day by gavage for 28 days modulates the immune response in female B6C3F1 mice by increasing the number, percentage, and activity of NK cells, both Con A and anti-CD3 antibody-mediated T-cell proliferation, the number of neutrophils, and the host resistance to $L.$ $monocytogenes$ infection. On the other hand, B cell activity was decreased at the 800 mg/kg dose level. In conclusion, CLT produced an enhancement of host resistance to $Listeria$ $monocytogenes$ infection possibly through modulating the function of neutrophils, T cells and NK cells in female B6C3F1 mice using a T regulatory cell-related mechanism.

Immunomodulation in female B6C3F1 mice following treatment with Chai-Ling-Tang, a formula consisting of twelve herbs

Tai L Guo,Rui P. Chi,Jonathan Fu,Kimber L. White Jr 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.2

Chai-Ling-Tang (CLT) is a traditional Chinese herbal decoction consisting of twelve different herbs, and it has been used to treat a number of immune related disorders. However, the mechanism for its clinical effectiveness was not fully understood. In this study, CLT’s effect on the immune system of female B6C3F1 mice was evaluated by examining the IgM antibody forming cell (AFC) response; natural killer (NK) cell activity; the expression of splenocyte surface markers; concanavalin (Con) A and anti-CD3 antibody-mediated T-lymphocyte proliferation; lipopolysaccharide-mediated-B cell proliferation, and the host resistances to the Listeria monocytogenes bacterial infection and B16F10 melanoma. The results from these studies suggest that the administration of CLT at doses between 400 and 800 mg/kg/day by gavage for 28 days modulates the immune response in female B6C3F1 mice by increasing the number, percentage, and activity of NK cells, both Con A and anti-CD3 antibody-mediated T-cell proliferation, the number of neutrophils, and the host resistance to L. monocytogenes infection. On the other hand, B cell activity was decreased at the 800 mg/kg dose level. In conclusion, CLT produced an enhancement of host resistance to Listeria monocytogenes infection possibly through modulating the function of neutrophils, T cells and NK cells in female B6C3F1 mice using a T regulatory cell-related mechanism.

In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response

Guo, Wei,Li, Guo-Jun,Xu, Hong-Bo,Xie, Jie-Shi,Shi, Tai-Ping,Zhang, Sheng-Zhong,Chen, Xiao-Hong,Huang, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.

남만지역 조선인회(1913~1931)의 설립과 변천

金泰國(Jin Tai Guo) 한국근현대사학회 2001 한국 근현대사 연구 Vol.17 No.-

청산리전쟁 전후 북간도지역 일본영사관의 동향과 그 성격

金泰國(Jin Tai-Guo) 한국사연구회 2000 한국사연구 Vol.111 No.-

Human HS1BP3 induces cell apoptosis and activates AP-1

( Tai Ping Shi ),( Jie Shi Xie ),( Ying Xiong ),( Wei Wei Deng ),( Jin Hai Guo ),( Feng Wang ),( Da Long Ma ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.6

In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription. [BMB reports 2011; 44(6): 381-386]

Down-regulation of lipocalin 2 contributes to chemoresistance in glioblastoma cells

Zheng, Long Tai,Lee, Shinrye,Yin, Guo Nan,Mori, Kiyoshi,Suk, Kyoungho Blackwell Publishing Ltd 2009 Journal of Neurochemistry Vol.111 No.5

<P>Abstract</P><P>Malignant gliomas are the most common primary brain tumor and have a poor clinical prognosis. 1, 3-Bis (2-chloroethyl)-1-nitrosourea (BCNU) is an alkylating agent that is commonly used in glioma therapy. However, BCNU chemotherapy often fails due to drug resistance. To gain better understanding of molecular mechanisms underlying the drug resistance of glioma, a BCNU-resistant variant (C6R) of C6 rat glioma cells was selected and characterized. The established C6R cells were resistant to BCNU-induced cell death and cell cycle arrest as confirmed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide reduction assay and flow cytometric analysis of DNA content. C6R cells showed an increased expression of common drug resistance-related genes such as O6-methylguanine-DNA methyltransferase and multiple drug resistance 1. In contrast, C6R cells showed a decreased expression of glial fibrillary acidic protein, therefore, displaying shorter cellular processes compared with parental C6 cells. More importantly, in conjunction with the morphological changes, the expression of lipocalin-2 (lcn2), a 25-kDa secreted proapoptotic protein, was markedly reduced in the BCNU-resistant C6R cells. However, there was no significant change in the expression of lcn2 receptors. Addition of recombinant LCN2 protein or introduction of lcn2 cDNA significantly increased the sensitivity of C6 cells and human glioma cells to BCNU or other anticancer drugs, while knockdown of lcn2 expression by antisense cDNA transfection decreased the sensitivity. When lcn2 was re-expressed in C6R cells, the BCNU sensitivity was restored. Lcn2 enhanced BCNU-induced Akt dephosphorylation providing a molecular basis of apoptosis sensitization. These results suggest that LCN2 protein may be involved in glioma drug resistance and may provide a new approach to sensitizing glioblastoma to chemotherapy.</P>

림와(林蛙)(Rana temporaria chinensis David) 피부세포(皮膚細胞)의 미세구조(微細構造)

로용태 ( Yong Tai Noh ),문인호 ( In Ho Moon ),박인봉 ( Ren Feng Piao ),엄창국 ( Chang Guo Yan ),김용훈 ( Long Xun Jin ) 건국대학교 기초과학연구소 1992 理學論集 Vol.17 No.-

中國 長白山에서 採集된 林蛙(Rana temporaria chinensis David)의 背部와 腹部 皮膚 組織을 pH 7.2에서 0.1M phosphate buffer로 緩衝시킨 2.5% glutaraldehyde와 2% osmium tetroxide에 前·後 固定하여, 各級 ethyl alcohol과 acetone으로 脫水, Epon 812 mixture에 包埋하여 LKB ultram-icrotome으로 超灌切片을 만들어 uranyl acetate와 lead citrate로 梁色하여 JEM100 CX-II 電子願微鏡으로 觀察하여 다음과 같은 結果를 얻었다. 1. 林蛙의 皮膚는 多層으로 種子層, 練細胞層, 顆粒層 및 角質層으로 構成되였는데 角質層은 완전히 角質化한 細胞와 semi-cornified cell도 있다. 2. 種子層 細胞는 背部와 腹部 공히 細胞質 대부분을 차지하는 rough endoplasmic reticulum이 他層과 區分되며, mitochondria, Golgi complex, 電子密度를 달리하는 顆粒 等이 發達하였으며 密集된 microvilli가 發達하였다. 3. 練細胞層은 眼部와 背部가 공히 多角形의 細胞로 이루어졌으며 他層에 비하여 desmosome이 發達하였고 細胞質 周邊部에는 tonofibril이 있다. 背部細胞는 願部 練細胞보다 많은 顆粒을 나타낸다. 4. 顆粒層은 內外 二層으로 構成되였고, 대부분의 細脫質들은 電子密度가 비슷하나, 內層에서 間歇的으로 電子密度가 낮은 細胞가 많이 있다. 5. 角質層은 角質細胞 사이에 微細管이 zig zag로 顆粒層까지 連結하였고 角質層에는 semi-cornified cell이 있다. 角質細胞質에는 microfilament, 細胞顆粒으로 차 있으며 멜라닌顆粒이 觀察된다. The authors observed the ultrastructure of the dorsal and ventral skin of the frog (Rana temporaria chinensis David) during the active phases. The specimens from the skin were fixed in 2.5% glutaraldehyde-paraformadehyde fixative in 0.1M phosphate buffer at pH7.2 prior to fixation in 2% osmium tetroxide, dehydrated in graded ethyl alcohol and acetone, embedded in Epon 812 mixture, and sectioned with LKB-Ultramicrotome. The ultrathin section were contrasted with uranyl acetate and lead citrate and observed with a JEOL-100cx-II electron microscope. The results were as follows. 1. Frog epidermis consisted stratified epithelium and was divided into four strata; stratum germinativum, stratum spinosum, stratum granulosum and stratum corneum. 2. Stratum germinativum: In the dorsal and ventral, the cells were columnar in form, rough endoplasmic reticula were specially developed in the whole cytoplasm and mitochondria, Golgi complex and granules were well developed in the cytoplasm. Many microvilli were aggregated densely in the intercellular space. 3. Stratum spinosum: In the dorsal and ventral, the cells were polygonal in form, and desmosome and tonofibrils were well developed in the pericytoplasm. In the dorsal cells were many granules more than the ventral cells. 4. Stratum granulosum: In the dorsal and ventral, it consisted of the inner and outer layers with granules of the same electron density, but intermittently in the inner layer with lower electron density. 5. Stratum corneum: In the dorsal and ventral, it was separated from the underling outer stratum granulosum by the subcorneal space and in the layers with zigzag channel to the underling outer stratum granulosum. Semi-cornified and conified cell were squamous in form.

중국산(中國産) 집먼지진드기류(類)(먼지진드기과(科))의 계통분류학적(系統分類學的) 연구(硏究)

로용태 ( Yong Tai Noh ),손병옥 ( Byung Ok Sohn ),박인봉 ( Ren Feng Piao ),엄창국 ( Chang Guo Yan ) 건국대학교 기초과학연구소 1992 理學論集 Vol.17 No.-

中國 吉林省 龍井市 延邊農學院 周邊의 가정집에서 1991年 7月에 收去한 집먼지로부터 採集된 138個體의 진드기類 中 먼지진드기科의 Dermatophagoides pteronyssinus (Trouessart, 1897)와 D. farinae Hughes, 1961가 同定·分類되었다. 이들 2種은 中國에서 처음으로 記錄된다. Dermatophagoides pteronyssinus (Trouessart, 1897) and D.farinae Hughes, 1961 were collected from house dust around Yanbian Argicul-tural College, Jilin in China during the period of July, 1991. They are newly recorded in China.