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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Cho, Woosum,Reddy, Saripally Sudhaker,Kim, Junyoung,Cho, Young-Rae,Jin, Sung-Ho The Royal Society of Chemistry 2018 Journal of Materials Chemistry C Vol.6 No.43
<P>A novel cross-linkable red iridium(iii) complex and electron transport material were designed and synthesized for use in the fabrication of solution-processed phosphorescent organic light-emitting diodes (PHOLEDs). The newly cross-linkable red Ir(iii) complex was successfully thermal cross-linked with a cross-linkable host in the emitting layer (EML). After cross-linking, the EML was found not to be damaged by organic solvents. A maximum external quantum efficiency (EQE) of 4.64% (2.77 cd A<SUP>−1</SUP>) was achieved for deep-red PHOLEDs after rinsing, which was similar to the EQE of 4.59% (2.52 cd A<SUP>−1</SUP>) after rinsing the deep-red PHOLEDs with thermal cross-linking.</P>
Optimum Tire Contour Design Using Systematic STOM and Neural Network
Cho, Jin-Rae,Jeong, Hyun-Sung,Yoo, Wan-Suk,Shin, Sung-Woo The Korean Society of Mechanical Engineers 2004 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.18 No.8
An efficient multi-objective optimization method is presented making use of neural network and a systematic satisficing trade-off method (STOM), in order to simultaneously improve both maneuverability and durability of tire. Objective functions are defined as follows: the sidewall-carcass tension distribution for the former performance while the belt-edge strain energy density for the latter. A back-propagation neural network model approximates the objective functions to reduce the total CPU time required for the sensitivity analysis using finite difference scheme. The satisficing trade-off process between the objective functions showing the remarkably conflicting trends each other is systematically carried out according to our aspiration-level adjustment procedure. The optimization procedure presented is illustrated through the optimum design simulation of a representative automobile tire. The assessment of its numerical merit as well as the optimization results is also presented.
( Sang Wook Lee ),( Myung Rae Cho ),( Ho Hyoung Lee ),( Won Kee Choi ),( Joo Hwan Lee ) 대한고관절학회 2015 Hip and Pelvis Vol.27 No.1
Purpose: To assess the frequencies and sites of surgical glove perforations in lower extremity fracture surgery and hip joint replacement (HJR) surgery. Additionally, we also studied the usefulness of an indicator system glove. Materials and Methods: We assessed surgical glove perforations in 30 cases of lower extremity fracture surgery and 18 cases of HJR surgery conducted by one right handed 1st operator from April 2013 to July 2013. We assessed frequencies and sites of perforation in 152 gloves; 95 used in lower extremity fracture surgery and 57 used in HJR surgery. We studied the perforation rates and sites according to participants and operation types. Using the Biogel indicator system glove, which is well known as a fast indicator of glove perforation, we were also able to assess the time difference between operative participant detection of perforation and inspector nurse detection while observing in the operative field. Results: There were 18 of 30 cases in lower extremity fracture surgeries and 12 of 18 cases in HJR surgeries which had more than one surgical glove perforation event. Of all 152 gloves used, perforation occurred in 15 of 57 gloves (26.3%) in HJR surgery and 23 of 95 gloves (24.2%) in lower extremity fracture surgery. Perforation occurred more frequently in operators than assistant doctors or scrub nurses. The most frequent perforation site was the second digit of the left hand. On average, the time difference between operative participant notice of perforation and inspector nurse notice of perforation was 20.6 seconds. Conclusion: The perforation of surgical gloves happened in approximately one out of every four persons. Importantly, we noted a 37.0% prevalence of glove perforation in 1st operators. Considering that glove perforation is a critical factor responsible for intra-operative infection, surgeons must be conscious of the risk of surgical glove perforation and use double gloving regularly. Furthermore, indicator double gloving is recommended for fast detection of outer glove perforation.
Cho, Sang-Rae,Kim, Ui-Hyung,Kumar, Kuldeep,Lee, Seung-Hwan,Lee, Myeung-Sik,Kim, Hyeon-Shup,Lee, Hyun-Jae,Yang, Byoung-Chul The Korean Society of Embryo Transfer 2014 한국동물생명공학회지 Vol.29 No.4
The objective of this study was to investigate the interrelationship of weight and hormonal changes through the administration of vitamin A to first time pregnant heifers. A total of 28 Korean heifers was used for this study. The heifers were divided into two groups - with vitamin A (n=14) and without vitamin A (control) (n=14) in the feed. Body weight increased in vitamin A treated heifers starting 9 months until 15 months. After pregnancy, vitamin A treated heifers were found to maintain higher body weights than the control group. Pre-pregnancy and post pregnancy progesterone levels were not different between the two groups. Serum estradiol levels of heifers at different growth stages showed relatively higher E2 levels than the control. Also, the control during pregnancy may show higher serum E2 levels than the vitamin A treated heifers. The growth phase serum estradiol levels in heifers may be relatively higher than the control. During pregnancy it showed a similar trend. Serum levels of vitamin A treated heifers did not differ from pregnant heifers at 5 months of age. However, after 5 months from conception until 8 months of treatment it showed a high level. Serum cholesterol in pregnant cows was higher in the control group than the treatment from beginning until the end of pregnancy. This is considered to be related to fetus development during pregnancy, as well as the mother's health.
Cho, Eun Jeong,Chun, Sung-Min,Park, Hwan,Sung, Chang Ohk,Kim, Kyu-Rae Elsevier 2020 Gynecologic oncology Vol.157 No.1
<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>Genomic characteristics of gestational trophoblastic neoplasm (GTN) are mostly unknown. This study reveals the molecular features of malignant GTN, including choriocarcinoma (CC), epithelioid trophoblastic tumor (ETT), and placental site trophoblastic tumor (PSTT), by whole transcriptome sequencing analysis.</P> <P><B>Methods</B></P> <P>Data obtained from the total RNA sequencing of 2 CC, 4 ETT, and 4 PSTT were evaluated for differential gene expression, pathway alteration, fusion gene, infiltrating immune cell type, PD-L1 and <I>PTEN</I> expression level, and mutation analysis was performed.</P> <P><B>Results</B></P> <P>The transcriptome data were correlated with known biomarkers, including <I>HDS3B1</I>, p63, hCG, and hPL for all tumor types. ETT and PSTT were more closely clustered compared with CC in clustering analysis using gene expression; however, ETT showed various altered signaling pathways, including PI3K-Akt-mTOR, with frequent loss of PTEN protein expression. This finding was both well correlated with <I>PIK3CA</I> c.3140A > G pathogenic mutation, detected in 1 ETT, and further confirmed using the MassARRAY method. PSTT showed an overexpressed gene cluster associated with muscle contraction and G protein-coupled receptor activity. No significant fusion gene was seen in all 10 cases. In tumor-infiltrating immune cell profiles, CD4 memory T cell and macrophage signature were relatively high in ETT and PSTT. PD-L1 mRNA expression level was high in all cases, which was significantly correlated with the PD-L1 level by immunohistochemistry (<I>p</I> = 0.03) with positivity in all 10 cases.</P> <P><B>Conclusions</B></P> <P>ETT and PSTT were similar at the transcriptome level, with a high level of PD-L1 expression in all tumor types; however, specific pathways, such as PI3K signaling, were altered in ETT.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ETT and PSTT are closely linked in whole transcriptome level. </LI> <LI> ETT is altered in PI3K-AKT-mTOR signaling pathway. </LI> <LI> ETT harbors <I>PIK3CA</I> mutation. </LI> <LI> ETT, PSTT, and CC show a variable high expression level of PD-L1. </LI> </UL> </P>
Sung-Sik Kang,Ui-Hyung Kim,Jun Sang Ahn,Jeong Il Won,Sang-Rae Cho 한국동물생명공학회(구 한국동물번식학회) 2021 Journal of Animal Reproduction and Biotechnology Vol.36 No.2
In the present study, we examined if deep uterine artificial insemination (DUAI) can improve the pregnancy rate of artificial insemination (AI) using epididymal spermatozoa (ES) in Hanwoo cattle. The estrus cycles of 88 Hanwoo cows were synchronized, and 17 cows were artificially inseminated using the DUAI method with ES, 20 cows were artificially inseminated via the uterine body (BUAI) method with ES, and as a control, 51 cows were inseminated by using the BUAI method with ejaculated spermatozoa from 1 proven bull after frozen thawing. The pregnancy rate of the DUAI method (58.8%) was higher than that of the BUAI method (25.0%, p = 0.0498). The motility of ES was examined immediately after thawing and after 3 and 6 h of incubation. The rapid progressive sperm motility of the control group was significantly higher than that of the ES group immediately after thawing and after 3 and 6 h of incubation (p < 0.05). The straight line velocity and average path velocity of the ES group after 6 h of incubation were significantly lower than those in the control group (p < 0.05). The linearity and amplitude of lateral head of ES were lower than those at 6 h (p < 0.05). The flagellar beat cross frequency and hyperactivation of ES were lower than the control spermatozoa immediately after thawing and at 3 h (p < 0.05). These motility parameters suggested that ES had a low motility and fertilization ability compared to the control spermatozoa. After frozen-thawing and 3 h of incubation, the percentage of live spermatozoa with intact acrosomes in the ES was significantly lower than that in ejaculated spermatozoa (p < 0.05). Our findings suggested that the DUAI method can overcome the low pregnancy rate of ES, despite the low motility, viability, and fertilization ability of ES.