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KO, Sang-Hwan,LEE, Geun-Shik,VO, Thuy T. B.,JUNG, Eui-Man,CHOI, Kyung-Chul,CHEUNG, Ki-Wha,KIM, Jae Wha,PARK, Jong-Gil,OH, Goo Taeg,JEUNG, Eui-Bae Society for Reproduction and Development 2009 Journal of Reproduction and Development Vol.55 No.2
<P>The effect(s) of oral calcium and vitamin D<SUB>3</SUB> were examined on the expression of duodenal and renal active calcium transport genes, i.e., calbindin-D9k (<I>CaBP-9k</I>) and calbindin-D28k (<I>CaBP-28k</I>), transient receptor potential cation channels (<I>TRPV5</I> and <I>TRPV6</I>), Na<SUP>+</SUP>/Ca<SUP>2+</SUP> exchanger 1 (<I>NCX1</I>) and plasma membrane calcium ATPase 1b (<I>PMCA1b</I>), in <I>CaBP-9k</I> KO mice. Wild-type (WT) and KO mice were provided with calcium and vitamin D<SUB>3</SUB>-deficient diets for 10 weeks. The deficient diet significantly decreased body weights compared with the normal diet groups. The serum calcium concentration of the WT mice was decreased by the deficient diet but was unchanged in the KO mice. The deficient diet significantly increased duodenal transcription of <I>CaBP-9k</I> and <I>TRPV6</I> in the WT mice, but no alteration was observed in the KO mice. In the kidney, the deficient diet significantly increased renal transcripts of <I>CaBP-9k</I>, <I>TRPV6</I>, <I>PMCA1b</I>, <I>CaBP-28k</I> and <I>TRPV5</I> in the WT mice but did not alter calcium-relating genes in the KO mice. Two potential mediators of calcium-processing genes, vitamin D receptor (VDR) and parathyroid hormone receptor (PTHR), have been suggested to be useful for elucidating these differential regulations in the calcium-related genes of the KO mice. Expression of VDR was not significantly affected by diet or the KO mutation. Renal <I>PTHR</I> mRNA levels were reduced by the diet, and reduced expression was also seen in the KO mice given the normal diet. Taken together, these results suggest that the active calcium transporting genes in KO mice may have resistance to the deficiency diet of calcium and vitamin D<SUB>3</SUB>.</P>
Ko, Kyoungtae,Kim, Sung Won,Lee, Hong-Jin,Hwang, In Gul,Kim, Bok Chul,Kee, Won-Seo,Kim, Young-Seog,Gihm, Yong Sik Elsevier 2017 Sedimentary geology Vol.358 No.-
<P><B>Abstract</B></P> <P>The Cretaceous Beolgeumri Formation is composed of laminated mudstones intercalated with sandstones, chert, and a bed of lapilli tuff that were deposited in a lacustrine environment at the terminal part of a regional strike-slip fault systems on the southwestern Korean Peninsula. The Beolgeumri Formation contains various types of soft sediment deformation (SSD) structures that are characterized by a wide extent (<4km), lateral continuity (<200m), and vertical repetition. The SSD structures can be classified into six categories based on their morphological features and deformation styles: 1) fold structures, 2) load structures, 3) water-escape structures, 4) rip-down structures, 5) boudin structures, and 6) synsedimentary fault structures. Field examination of SSD structures together with an analysis of the sedimentological records of the Beolgeumri Formation indicate that the SSD structures formed largely by liquefaction and/or fluidization triggered by ground shaking during earthquakes. To constrain the timing of the development of SSD structures in the Beolgeumri Formation, we conducted sensitive high-resolution ion microprobe (SHRIMP) U-Pb zircon age dating of block sized lithic clasts bearing volcaniclastic deposits that conformably underlie (the Mangryeongbong Tuff) and overlie (the Ttandallae Tuff) the Beolgeumri Formation. The Mangryeongbong and Ttandallae Tuffs have ages of 86.63±0.83Ma and 87.24±0.36Ma, respectively, indicating that the Beolgeumri Formation was deposited during a short interval between major volcanic eruptions. The large lithic clasts of volcaniclastic deposits suggest that the Beolgeumri Formation was deposited adjacent to an active volcanic edifice(s). Syndepositional magmatic activities are suggested by the occurrence of a lapilli tuff bed in the Beolgeumri Formation and an igneous intrusion (intermediate sill) that is crosscut by a sand dike, as well as the similar age results of the underlying and overlying volcaniclastic deposits. Thus, we infer that the earthquakes that caused the development of SSD structures in the study area were closely related to syndepositional magmatic activities, as is the case for modern tectonic earthquakes around active volcanoes.</P>
Ko, Eun-Ju,Lee, Young-Tae,Kim, Ki-Hye,Lee, Youri,Jung, Yu-Jin,Kim, Min-Chul,Lee, Yu-Na,Kang, Taeuk,Kang, Sang-Moo American Association of Immunologists 2017 Journal of Immunology Vol. No.
<P>Vaccine adjuvant effects in the CD4-deficient condition largely remain unknown. We investigated the roles of combined monophosphoryl lipid A (MPL) and aluminum hydroxide (Alum) adjuvant (MPL+Alum) in inducing immunity after immunization of CD4 knockout (CD4KO) and wild-type (WT) mice with T-dependent influenza vaccine. MPL+ Alum adjuvant mediated IgG isotypeswitched Abs, IgG-secreting cell responses, and protection in CD4KO mice, which were comparable to those in WT mice. In contrast, Alum adjuvant effects were dependent on CD4(+) T cells. MPL+ Alum adjuvant was effective in recruiting monocytes and neutrophils as well as in protecting macrophages from Alum-mediated cell loss at the injection site in CD4KO mice. MPL+Alum appeared to attenuate MPL-induced inflammatory responses in WT mice, likely improving the safety. Additional studies in CD4-depleted WT mice and MHC class II KO mice suggest that MHC class II+ APCs contribute to providing alternative B cell help in the CD4-deficient condition in the context of MPL+Alum-adjuvanted vaccination.</P>
Ko, In Ok,Jung, Ki-Hye,Kim, Mi Hyun,Kang, Kyeung Jun,Lee, Kyo Chul,Kim, Kyeong Min,Noh, Insup,Lee, Yong Jin,Lim, Sang Moo,Kim, Jung Young,Park, Ji-Ae Hindawi 2017 Contrast media and molecular imaging Vol.2017 No.-
<P>The thymidine analogue 3′-deoxy-3′-[<SUP>18</SUP>F]fluorothymidine, or [<SUP>18</SUP>F]fluorothymidine ([<SUP>18</SUP>F]FLT), is used to measure tumor cell proliferation with positron emission tomography (PET) imaging technology in nuclear medicine. FLT is phosphorylated by thymidine kinase 1 (TK1) and then trapped inside cells; it is not incorporated into DNA. Imaging with <SUP>18</SUP>F-radiolabeled FLT is a noninvasive technique to visualize cellular proliferation in tumors. However, it is difficult to distinguish between [<SUP>18</SUP>F]FLT and its metabolites by PET imaging, and quantification has not been attempted using current imaging methods. In this study, we successfully acquired<I> in vivo </I><SUP>19</SUP>F spectra of natural or nonradioactive 3′-deoxy-3′-fluorothymidine ([<SUP>19</SUP>F]FLT) and its monophosphate metabolite (FLT-MP) in a tumor xenograft mouse model using 9.4T magnetic resonance imaging (MRI). This preliminary result demonstrates that <SUP>19</SUP>F magnetic resonance spectroscopy (MRS) with FLT is suitable for the<I> in vivo</I> assessment of tumor aggressiveness and for early prediction of treatment response.</P>
( Sung Jun Ko ),( Sun Mi Choi ),( Jin Woo Lee ),( Young Sik Park ),( Chang Hoon Lee ),( Jae Joon Yim ),( Chul Gyu Yoo ),( Young Whan Kim ),( Sung Koo Han ),( Sang Min Lee ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.0
Background: Adipose tissue is recently recognized as not only energy reservoir but also endocrine organ producing proinflammatory cytokines. Especially the impact of visceral adipose tissue(VAT) in critical illnesses has been proposed, but researches on the association between VAT and sepsis were scarce and quantitative measurement of VAT had not been used. Methods: In this retrospective cohort study, we enrolled patients with sepsis who examined abdominal computed tomography( A-CT) within 1 month of occurrence of sepsis, among the patients admitted to intensive care unit(ICU). Age, sex, anthropometric values, comorbidities and APACHE II score were reviewed. The areas of VAT and total adipose tissue(TAT) on the section of A-CT image of the umbilicus level were measured by calculating pixels presenting fat density. Results: Among 287 patients admitted to ICU due to sepsis, 178 patients were included for this study. Median age was 65 and 59.0% were men. In-hospital mortality rate was 57.9%. Women had higher TAT and subcutaneous adipose tissue, lower VAT/TAT ratio compared to men. The amount of VAT and VAT/TAT ratio were higher in in-hospital mortality group than in survivor group(90.41cm2 vs. 63.83cm2 and 45.88% vs. 32.79%, p=0.001 and <0.001, respectively). After adjusting age, sex, APACHE II score and comorbidities, multivariate logistic regression analysis revealed that the amount of VAT and VAT/TAT ratio were independent prognostic factors of sepsis with obvious dose-dependent relationship(VAT/TAT ratio quartile 3: OR 8.832, p<0.001 and quartile 4: OR 29.477, p<0.001, compared to quartile 1 respectively). Conclusion: The amount of VAT and VAT/TAT ratio quantitatively measured by A-CT were positively correlated with mortality in sepsis, and this association was dose-dependent. Visceral obesity should be considered as the poor prognostic factor of sepsis.
Ko, Jeong Huy,Park, Rae-su,Jeon, Jong-Ki,Kim, Do Heui,Jung, Sang-Chul,Kim, Sang Chai,Park, Young-Kwon Elsevier 2015 Journal of industrial and engineering chemistry Vol.32 No.-
<P><B>Abstract</B></P> <P>Deactivated waste activated carbon due to the deposition of boron in the exhaust gas from an incinerator was regenerated by the treatment with surfactant, HCl, and NH<SUB>3</SUB>. The catalysts were characterized using BET surface area, ICP, XPS and FT-IR spectra. Sequential treatment with surfactant and HCl removed the adsorbed boron very effectively. NH<SUB>3</SUB> treatment increased the number of functional nitrogen groups in regenerated sample. For NH<SUB>3</SUB> selective catalytic reduction (SCR) of NO, sequential treatments with surfactant, HCl, and NH<SUB>3</SUB> showed the highest DeNOx activity, attributed to the increased metal removal efficiency, specific surface area and amount of nitrogen functional groups on the surface.</P>
호알칼리성 Bacillus sp. K-17 의 $\beta$-Xylosidase 유전자의 Subcloning 및 발현증진
Sung, Nack-Kie,Ko, Hack-Ryong,Kho, Yung-Hee,Chun, Hyo-Kon,Chung, Young-Chul 한국미생물 · 생명공학회 1989 한국미생물·생명공학회지 Vol.17 No.4
최초로 구축된 $\beta$-xylosidase 유전자 함유 5.0kb HindIII 절편을 포함하는 pAX278의 Insert 크기를 줄이기 위하여 subcloning을 행한 결과, 1.4kb EcoRI-XbaI 절편이 subcloning되었으며 이를 pAK 208로 명하였다. Plasmid pAX208에 의해 형질전환된 대장균은 $\beta$-xylosidase 활성이 pAX278의 경우보다 약 1.3배 증가하였고 또한, $\beta$-xylosidase의 활성을 증가시키기 위하여 강력한 tac-promoter를 가진 pKK223-3 plasmid vector를 이용하여 대장균에 cloning 및 발현시켰을 때, pAX278을 가진 대장균 형질전환체와 유전자 source균인 호알칼리성 Bacillus속 K-17에 비하여 각각 약 3.3배 및 1.8배의 효소활성 증가를 나타내었다. 전체 5.0kb HindIII 절편을 cloning하여 Bacillus속 K-17에 발현시킨 균주는 각각 3.7배 및 2.0배의 $\beta$-xylosidase 활성증가를 보였다. 각 형질전환체로부터 정제된 $\beta$-xylosidase의 효소학적 특성은 Bacillus속 K-17과 거의 일치하였다. To reduce the size of 5.0kb HindIII fragment containing $\beta$-xylosidase gene, the 5.0kb insert of pAX278 which was previously cloned was reduced by various deletions and thus 1.4kb EcoRI-Xbal fragment was subcloned into pUC19, and the recombinant plasmid was named pAK208. The $\beta$-xylosidase acnivity of E. coli harboring pAK208 was higher about 1.3times than that of pAX278. For the improvement of $\beta$-xylosidase activity, we cloned and expressed the $\beta$-xylosidase gene in E. coli using vector pKK223-3 containing a potent tac-promoter, and enzyme activity of the transformant harboring pKHR212 was increased about 3.3 and 1.8 times than that of E. coli(pAX278) and Bacillus sp. K-17, respectively. To obtain better expression of $\beta$-xylosidase gene, the whole 5.0kb HineIII fragment was recloned into pC194, and the Bacillus sp. K-17 transformant harbor-ing the recombinant plasmid pCX174 showed higher activity than that of the E. coli (pAX278) and Bacillus sp. K-17, respectively. The characteristics of enzyme purified from transformants were consistent with those front alkalophilic Bacillus sp, K-17.