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LiYong Chen,Jing Zhou,JiaJun Zhao,FuRong Wang,XiangLan Sun,Ling Gao,YuLian Jiao,XiaoLei Hou,ChengYong Qin 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.3
Chronic and heavy alcohol consumption is one of the causes of heart diseases. However, the effects of ethanol on insulin sensitivity in myocardium has been unclear. To investigate the effects of ethanol on the expression of AMP-activated protein kinase (AMPK), myocyte enhancer factor 2 (MEF2) and glucose transporter 4 (GLUT4), all of which are involved in the regulation of insulin sensitivity, in the myocardium, we performed three parts of experiments in vivo and in vitro. I: Rats were injected with 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR, 0.8 mg.kg-1) for 2 h. II:Rats received different dose (0.5, 2.5 or 5 g.kg-1.d-1) of ethanol for 22-week. III: Primary neonatal rat cardiomyocytes were isolated and treated with or without 100 mM ethanol or 1 mM AICAR for 4 h. The cardiac protein and mRNA expression of AMPKα subunits, MEF2and GLUT4 were observed by western-blotting and RT-PCR, respectively. Serum TNFα levels were assessed by ELISA. The results showed chronic ethanol exposure induced insulin resistance. Ethanol decreased the mRNA levels of AMPKα1 and α2, the protein levels of total- and phospho-AMPKα in cardiomyocytes. Similarly, ethanol showed inhibitory effects on both the mRNA and protein levels of MEF2A and 2D,and GLUT4 in a dose-response-like fashion. Correlation analysis implied an association between phospho-AMPKα and MEF2A or MEF2D, and between the levels of MEF2 protein and GLUT4 transcription. In addition,ethanol elevated serum TNFα level. Taken together,chronic ethanol exposure decreases the expression of AMPKα and MEF2, and is associated with GLUT4 decline in rat myocardium.
Liu Xu,Sun Liyong,Nie Tangjie,Chen Yao,Yin Zengfang 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.3
Dendrobium moniliforme is a threatened and medicinal orchid species in China. The establishment of in vitro rapid propagation technology system will facilitate preservation and utilization of germplasm resources in D. moniliforme. In this study, the effects of different plant growth regulators (PGRs) on seed germination, proliferation of cluster shoots, rooting of shoots were tested under asymbiotic culture condition, and the best acclimatization condition of plantlets was screened. Our research results were shown as follows: germination rate (GR) was significantly increased by adding N6-benzyladenine (BA, 0.1 mg/L) during asymbiotic culture. In Murashige and Skoog (MS) medium supplemented with α-naphthaleneacetic acid (NAA, 1.0 mg/L) and BA (1.0 mg/L), the proliferation coefficient of cluster shoots was 6.1 times comparing with the control group. The MS medium with NAA (0.5 mg/L) and indole-3-butyric acid (IBA, 0.5 mg/L) promoted the highest rooting rate (RR). For plantlet transplanting, the equal mixing volume ratio of pine bark, turfy soil, and peanut shells was the best substrate. In a word, this rapid propagation system provides strong technical support for D. moniliforme to expand proliferation and germplasm resource protection.