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새로운 생명공학작물 안전성 평가를 위한 작물 성분 DB 구축
김은하,이성곤,박수윤,이상구,오선우,Kim, Eun-Ha,Lee, Seong-Kon,Park, Soo-Yun,Lee, Sang-Gu,Oh, Seon-Woo 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.4
국립농업과학원 생물안전성과는 생명공학기술로 개발되는 작물(GM작물)의 안전성 평가 중 '성분분석에 의한 비교평가'에 참고자료로 활용할 수 있도록 기존 상업화 작물성분의 분석 데이터를 제공하는 "작물성분 DB"를 구축하였다. "작물성분 DB"는 우리나라의 자연 환경에서 재배되는 주요 작물 품종들의 영양성분 함량정보를 제공함으로써 품종과 재배 지역, 연도에 따라 함량의 변화 정도를 확인할 수 있도록 데이터를 업그레이드 하고 있다. 현재 2곳 이상의 재배지역에서 2년 이상 재배한 벼와 고추 시료에 대한 주요 영양분과 항영양소, 2차 대사산물을 검증된 분석 방법을 사용하여 분석한 데이트를 제공하고 있다. 데이터는 평균값과 최저, 최대값을 제공함으로써 GM작물의 안전성 평가시 GM작물과 대응 비교작물 간에 나타나는 통계적 차이가 기존 상업작물의 생물학적 차이 또는 허용범위 내에 속하는지를 평가할 수 있도록 하였다. "작물성분 DB"는 사용자가 선택한 쿼리를 기반으로 쉽게 검색하고 활용하도록 하고 있다. 또한 유색미와 감자, 고구마, 콜리플라워와 같은 유색 작물의 기능성 성분 함량 정보를 제공하고 있어 일반 소비자들도 유용하게 정보를 활용할 수 있다. 본 논문은 상업작물 성분에 대한 중요한 정보를 제공하는 농진청 "작물성분 DB"의 구성과 사용법을 소개하였다. The Biosafety Division of the National Academy of Agricultural Science has developed a 'Crop Composition DB' that provides analytical data on commercialized crops. It can be used as a reference in the 'Comparative Evaluation by Compositional Analysis' for the safety assessment of genetically modified (GM) crops. This database provides the composition of crops cultivated in Korea, and thus upgrades the data to check the extent of changes in the compositional content depending on the cultivated area, varieties and year. The database is a compilation of data on the antioxidant, nutrient and secondary metabolite compositions of rice and capsicum grown in two or more cultivation areas for a period of more than two years. Data analysis was conducted under the guidelines of the Association of Official Analytical Chemists or methods previously reported on papers. The data was provided as average, minimum and maximum values to assess whether the statistical differences between the GM crops and comparative non-GM crops fall within the biological differences or tolerances of the existing commercial crops. The Crop Composition DB is an open-access source and is easy to access based on the query selected by the user. Moreover, functional ingredients of colored crops, such as potatoes, sweet potatoes and cauliflowers, were provided so that food information can be used and utilized by general consumers. This paper introduces the feature and usage of 'Crop Composition DB', which is a valuable tool for characterizing the composition of conventional crops.
오성덕(Sung-Dug Oh),박수윤(Soo-Yun Park),이시명(Si Myung Lee),이기종(Kijong Lee),손수인(Soo-In Sohn),박순기(Soon Ki Park),류태훈(Tae-Hun Ryu) 한국육종학회 2015 한국육종학회지 Vol.47 No.1
The β-carotene biofortified transgenic rice was developed by transforming rice cv. Nakdongbyeo with phytoene synthase (Psy) and carotene desaturase (Crt I) genes isolated from Capsicum and Pantoea. The aim of this study was to perform molecular characterization of rice transformants of T5-T7 generation harboring Psy and Ctr I genes driven by endosperm specific globulin promoter for biosafety evaluation of β-carotene biofortified transgenic rice. The structure and sequence of T-DNA in the transformation vector and the insertion sites, flanking sequences and generational stability of inserted T-DNA in transgenic rice lines were analyzed. The transformation vector consisted of right border, MAR gene, carotenogenic genes unit, herbicide resistance selectable marker unit, MAR gene and left border in sequential order. T-DNA was introduced at the position of 30,363,938-30,363,973 bp of chromosome No. 2 by adaptor-ligation PCR. Stable integration of T-DNA and stable expression of bar gene was confirmed in T5 to T7 generations. It was also confirmed that the backbone DNA of transformation vector containing antibacterial gene was not present in the genome of β-carotene biofortified transgenic rice. HPLC analysis confirmed that carotenoids were consistently detected through T5-T7 generations.
해충저항성 Agb0101 벼 알레르기 유발 가능성 평가 연구
이시명 ( Sim Yung Lee ),박수윤 ( Soo Youn Park ),여윤수 ( Yun Soo Yeo ),조현석 ( Hyun Suk Cho ),권순종 ( Sun Jong Kwon ),오성덕 ( Sung Duk Oh ),오선우 ( Sun Woo Oh ),김재광 ( Jaek Wang Kim ),박순기 ( Soon Ki Park ) 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.1
Genetically modified crops are becoming an increasingly important feature of the agricultural landscapes. Specially, insect-resistant GM crops are the most fast growing traits in the world. We developed Insect-resistant Agb0101 rice using the cry1ac1 gene in order to support the food safety assessment of insect-resistant Agb0101 rice containing modified Cry1Ac1 (mCry1Ac1) protein. The novel protein introduced into the genetically modified crops need to be evaluated for the potential allergenecity before their introduction into the food chain to address the safety concerns of consumers for commercialization. We performed assessment for allergy. The mCry1Ac1 protein does not possess the characteristics associated with food allergens, and no posttranslational change. The mCry1Ac1 protein can be rapidly degraded in gastric and intestinal fluids and heat treatment at 100oC. In conclusion, there is a reasonable certainty of no harm resulting from the inclusion of the mCry1Ac1 protein in human food or animal feed.
이소영(So-Young Lee),여윤수(Yunsoo Yeo),박수윤(Soo-Yun Park),오선우(Seon-Woo Oh),윤은경(Eun Kyung Yoon),신공식(Kong-Sik Shin),우희종(Hee-Jong Woo),임명호(Myung-Ho Lim) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
In order to assess the substantial equivalence of two varieties of genetically modified rice, herbicide-tolerant Ab rice and the insect-resistant Bt rice, to the non-GM Dongjin-byeo cultivar. We analyzed the compositions and contents of the proximate, amino acids, minerals, fatty acids, vitamins, and anti-nutrients in their unpolished grains using t-test (p<0.05). A comparison of fatty acids compositions showed that the levels of stearic acid and arachidonic acid in Ab rice and those of myristic, palmitic, oleic, linoleic, and gadoleic acid in Bt rice were different significantly from the corresponding levels in Dongjin-byeo. Vitamin content did not differ between Bt and Dongjin-byeo, but the content of vitamins B1, B7, and E in Ab rice differed from that in Dongjin-byeo. Iron content in Ab and Bt rice was 2 times higher than that in Dongjin-byeo, although it was within the reference range set by Codex. The amount of the anti-nutrient trypsin inhibitor was 0.1 TIU/mg in the unpolished grain of all three rice varieties examined. Of the 47 components analyzed, 17 were significantly different among the three rice varieties; however, most of these differences were within the Codex reference range for commercial rice. Overall, it was confirmed that both Bt and Ab rice are substantially equivalent to the Dongjin-byeo and other commercial varieties of rice.
오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),박수윤 ( Soo Yun Park ),신혜철 ( Hye Chul Shin ),손수인 ( Soo In Sohn ),박종석 ( Jong Sug Park ),류태훈 ( Tae Hun Ryu ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.1
비타민A 강화벼와 ‘낙동’의 미꾸리(Misgurnus anguillicaudatus)와 잉어(Cyprinus carpio)에 대한 급성독성시험을 실시한 결과 48시간 및 96시간-LC50은 1,000 mg/L이상으로 나타났다. 48시간 및 96시간 무영향농도(NOEC)는 1,000 mg/L이었다. 급성독성 시험기간 중 비타민A 강화벼와 낙동벼간의 pH, DO, 수온, 체중 및 전장에 대한 유의적인 결과는 나타나지 않았다. The carotenoid-biofortified (PAC) rice was developed and confirmed biosynthesis of specific carotenoids (zeaxanthin, a-carotene and β-carotene) in rice endosperm. Also, PAC rice contained herbicide resistance selectable marker by introducing the phosphinothricin acetyltransferase (PAT) gene from the Streptomyces hygroscopicus. For biosafety assessment, the PAC rice expressing the herbicide resistant gene PAT and carotenoid biosynthesis gene PSY/CrtI was tested for the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. M. anguillicaudatus and C. carpio fed 100% ground rice of suspension, PAC or non-GM counterpart rice (Nakdong). Gene expression of PAC rice was confirmed to have the PAT by the southern blot and western blot analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between PAC rice and non-GM counterpart rice. The 96hr-LC50 values showed no difference between PAC rice(>1,000mg/L) and non-GM rice(>1,000 mg/L). We concluded that there was no significant difference in toxicity for non-target organisms(M. anguillicaudatus and C. carpio) between PAC rice and non-GM counterparts.
이상구(Sang-Gu Lee),오선우(Seon-Woo Oh),박수윤(Soo-Yun Park),박현민(Hyoun-Min Park),김은하(Eun-Ha Kim),진소라(So-Ra Jin),류태훈(Tae-Hun Ryu) 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.4
To ensure the safety of developing or importing genetically modified organisms (GMOs), Korea has enacted the “LMO Act.” Accordingly, the safety of using GMOs as food or feed is evaluated in accordance with the concept of “substantial equivalence” proposed by OECD. The allergenicity of GMOs is assessed as a part of their safety evaluation. The methods of allergenicity assessment have been discussed by various international organizations, such as the OECD, FAO, and WHO. The main methods used for the allergenicity assessment of proteins newly expressed in GMOs include assessment of the physicochemical stability of these proteins, evaluation of their amino acid homology with existing allergenic proteins, and serum screening. In this study, we describe guidelines and related studies for the allergenicity assessment of GM crops.
베타카로틴 강화 GM콩과 야생콩의 교잡종 종자 특성 평가
오성덕(Sung-Dug Oh),장예진(Ye-Jin Jang),박수윤(Soo-Yun Park),서상재(Sang Jae Suh),이범규(Bumkyu Lee) 한국잡초학회·한국잔디학회 2020 Weed & Turfgrass Science Vol.9 No.2
본 연구에서는 야생콩과 베타카로틴 강화콩의 교잡으로 형성된 교잡종의 종자 및 생육 특성과 월동성을 조사하여 교잡종의 잡초화 가능성을 평가하고자 하였다. 야생콩과 베타카로틴 강화 콩을 개화기 일치 등의 인위적으로 교잡한 결과 최종 교잡율은 0.21% 로, 8개체의 교잡종이 선발되었다. 교잡종은 생육 형태는 모본인 야생콩과 유사한 형태를 보였으며, 형성된 협수와 종자 수 및 종자의 크기는 야생콩과 베타카로틴 강화 콩의 중간 형질을 나타냈다. 반면, 종자의 발아 속도는 야생콩보다 크게 증가하여 베타카로틴 강화콩과 유사한 속도를 보였다. 교잡종은 표토조건에서 3개월간 월동한 경우 야생콩과 비슷한 수치의 월동성을 보였으나, 이를 제외한 나머지 조건에서는 야생콩보다 낮은 월동휴면성을 보였다. 베타카로틴 강화콩과 야생콩간의 낮은 교잡율과 월동휴면성을 바탕으로 교잡종이 환경 내 방출되어 생태계 교란을 유발할 가능성은 낮지만, 다양한 요인을 고려하여 장기적인 교잡 후대종의 도입 단백질 활성 평가 및 잡초화 가능성 평가 등을 비롯한 환경 안전성 평가가 필요할 것으로 사료된다. As the cultivation area for genetically modified (GM) crops is increasing continuously, the concerns about the environmental release of GM crops are also growing. Therefore, we conducted this study to verify gene flow from GM soybean, β-carotene enhanced transgenic soybean, to wild-type soybean and analyze the characteristic of hybrid soybean. The hybridization rate between β-carotene enhanced soybean and wild-type soybean was 0.21%. The hybrid soybean showed the highest value on stem length, number of branches and number of nodes than the β-carotene soybean and wild-type soybean. The seeds of hybrid soybean had intermediate characteristics between β-carotene enhanced soybean and wildtype soybean in seed length, seed width, seed weight and seed coat color. The germination time of hybrid soybean was faster than wild-type soybean while the wild-type was similar with β-carotene enhanced soybean. The hybrid soybean showed the lower over-wintering dormancy rates than wild-type soybean after 5 months, and the β-carotene soybean had extremely low or no dormancy rates on all conditions. These results indicate that the hybrid soybean has intermediate characteristics between their parents and will be useful for research in management of GM crops and hybrid crops.