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Plant Regeneration and Bulblet Formation of Allium wakegi Araki
Song,Won-seob,Yang,Deok-Chun,Yoon,Jae-Ho,Ryu,Sang-Hyun 한국자원식물학회 2004 Plant Resources Vol.7 No.1
Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.
A field study of colloid transport in surface and subsurface flows
Zhang, Wei,Tang, Xiang-Yu,Xian, Qing-Song,Weisbrod, Noam,Yang, Jae E.,Wang, Hong-Lan Elsevier, etc 2016 Journal of hydrology Vol.542 No.-
<P><B>Abstract</B></P> <P>Colloids have been recognized to enhance the migration of strongly-sorbing contaminants. However, few field investigations have examined combined colloid transport via surface runoff and subsurface flows. In a headwater catchment of the upper Yangtze River, a 6m (L) by 4m (W) sloping (6°) farmland plot was built by cement walls to form no-flow side boundaries. The plot was monitored in the summer of 2014 for the release and transport of natural colloids via surface runoff and subsurface flows (i.e., the interflow from the soil-mudrock interface and fracture flow from the mudrock-sandstone interface) in response to rain events. The water sources of the subsurface flows were apportioned to individual rain events using a two end-member model (i.e., mobile pre-event soil water extracted by a suction-cup sampler <I>vs</I>. rainwater (event water)) based on <I>δ</I> <SUP>18</SUP>O measurements. For rain events with high preceding soil moisture, mobile pre-event soil water was the main contributor (generally >60%) to the fracture flow. The colloid concentration in the surface runoff was 1–2 orders of magnitude higher than that in the subsurface flows. The lowest colloid concentration was found in the subsurface interflow, which was probably the result of pore-scale colloid straining mechanisms. The rainfall intensity and its temporal variation govern the dynamics of the colloid concentrations in both surface runoff and subsurface flows. The duration of the antecedent dry period affected not only the relative contributions of the rainwater and the mobile pre-event soil water to the subsurface flows but also the peak colloid concentration, particularly in the fracture flow. The <10μm fine colloid size fraction accounted for more than 80% of the total suspended particles in the surface runoff, while the colloid size distributions of both the interflow and the fracture flow shifted towards larger diameters. These results highlight the need to avoid the application of strongly-sorbing agrochemicals (e.g., pesticides, phosphorus fertilizers) immediately before rainfall following a long no-rain period because their transport in association with colloids may occur rapidly over long distances via both surface runoff and subsurface flows with rainfall.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Subsurface flow was apportioned into rainwater and mobile pre-event soil water. </LI> <LI> The duration of no-rain period affects peak colloid concentration. </LI> <LI> Rainfall intensity and its temporal variation govern colloid discharge dynamics. </LI> </UL> </P>
Thoracoscopic Resection of the First Rib for Thoracic Outlet Syndrome: A Case Report
( Jae Gul Kang ),( Soon-ho Chon ),( Kilsoo Yie ),( Min Koo Lee ),( Oh Sang Kwon ),( Song Hyun Lee ),( June Raphael Chon ) 대한외상학회 2017 大韓外傷學會誌 Vol.30 No.2
Standard open procedures for resection of the first rib in thoracic outlet syndrome can prove to be quite difficult with extensive incisions. A minimal invasive procedure can also be painstaking, but provides an attractive alternative to the more radical open procedures. We report the details of the technique with direct video footage of the procedure performed in a 41-year-old man with thoracic outlet syndrome done entirely by thoracoscopic methods. [ J Trauma Inj 2017; 30: 63-65 ]
Genetic and epigenetic analysis of the <i>EPHB2</i> gene in gastric cancers
SONG, JAE HWI,KIM, CHANG JAE,CHO, YONG GU,KWAK, HYUN JUNG,NAM, SUK WOO,YOO, NAM JIN,LEE, JUNG YOUNG,PARK, WON SANG Wiley-Blackwell Munksgaard 2007 APMIS Vol. No.
<P>EPHB2 is a member of the Eph receptor tyrosine kinase family and a direct transcriptional target of &bgr;-catenin/TCF. EPHB2 plays an important role in maintaining the correct positioning of the proliferative compartment in the crypt-villous axis. A loss of EPHB2 expression has been observed in human tumors, particularly in colonic adenomas and carcinomas. A search was made for mutations at the A9 tract in exon 17, an allelic loss at the <I>EPHB2</I> gene locus, and promoter hypermethylation of the <I>EPHB2</I> gene in 81 sporadic gastric cancers in order to determine if genetic or epigenetic alterations of the <I>EPHB2</I> gene are involved in the development and/or progression of gastric cancer. Unexpectedly, no frameshift mutation was found and there was a low frequency (20.8%) of allelic loss. In addition, promoter hypermethylation was detected in only one gastric cancer tissue sample. Therefore, genetic or epigenetic alterations of the <I>EPHB2</I> gene might be an uncommon event in the development or progression of gastric cancers.</P>
Song, Jae-Hoon,Cui, Chenglin,Kim, Seong-Kyun,Kim, Byung-Sung,Nam, Sangwook IEEE 2016 IEEE microwave and wireless components letters Vol.26 No.7
<P>In this letter, a 77-GHz transmitter (TX) with a 12.8-GHz phase-locked-loop (PLL) and a x6 frequency multiplier is presented for a FMCW radar sensor in a 65-nm CMOS process. To realize the low-phase-noise TX, a voltage controlled oscillator (VCO) with an excellent phase noise performance at a lower fundamental frequency (12.8 GHz) is designed and scaled up (x6) for the desired target frequency (77 GHz). The measured FMCW modulation range with an external triangular chirp signal (1-ms sweep time) is 601 MHz. The output power and the total DC power consumption of the TX are 8.9 dBm and 116.7 mW, respectively. Here, a good phase noise level of -91.16 dBc/Hz at a 1-MHz offset frequency from a 76.81-GHz carrier is achieved.</P>
Effects of Mizoribine on MHC-Restricted Exogenous Antigen Presentation in Dendritic Cells
Song, Young-Cheon,Han, Shin-Ha,Kim, Hyun-Yul,Kim, Kwang-Hee,Kwon, Jeung-Hak,Lee, Sang-Jin,Ha, Nam-Joo,Lee, Young-Hee,Lee, Chong-Kil,Kim, Kyung-Jae The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.12
Mizoribine (MZR) has been shown to possess immunosuppressive activity that selectively inhibits the proliferation of lymphocytes by interfering with inosine monophosphate dehydrogenase. The efficacy of MZR is not only in patients who have had renal transplantation, but also in patients with rheumatoid arthritis (RA), lupus nephritis, and primary nephritic syndrome. Because the exact mechanism of its immunosuppressive action is not clear, the object of this study was to examine the ability of MZR to regulate the antigen presenting cells (APCs), dendritic cells (DCs). In this work, we tested whether MZR ($1{\sim}10\;{\mu}g/mL$) could inhibit the cross-presentation of DCs. DC2.4 cells ($H-2K^{b}$) or bone marrow-derived DCs (BM-DCs) generated from BM cells of C57BL/6 mouse ($H-2K^{b}$) were cultured in the presence of MZR with OVA-microspheres, and the amount of OVA peptide-class I MHC complexes was measured by a T cell hybridoma, B3Z, that recognizes OVA (257-264 : SIINFEKL)-$H-2K^{b}$ complex and expresses-galactosidase. MZR profoundly inhibited the expression of SIINFEKL-$H-2K^{b}$ complexes. This inhibitory activity of MZR appeared to affect the phagocytic activity of DCs. MZR also decreased IL-2 production when we examined the effects of MZR on $CD4^{+}$ T cells. These results provide an understanding of the mechanism of immunosuppressive activity of MZR on the inhibition of MHC-restricted antigen presentation and phagocytic activity in relation to their actions on APCs.