http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Porcine epidemic diarrhea (PED) infection, diagnosis and vaccination: A mini review
Sekhon, Simranjeet Singh,Nguyen, Phat-Loc,Ahn, Ji-Young,Lee, Kyeong-Ah,Lee, Lyon,Kim, Sang Yong,Yoon, Hobaek,Park, Jihoo,Ko, Jung Ho,Kim, Yang-Hoon Springer-Verlag 2016 Toxicology and Environmental Health Sciences Vol.8 No.5
Structural and biochemical characterization of the cytosolic wheat cyclophilin TaCypA-1.
Sekhon, Simranjeet Singh,Kaur, Harsimran,Dutta, Tanima,Singh, Khushwant,Kumari, Sumita,Kang, Sunghyun,Park, Sung Goo,Park, Byoung Chul,Jeong, Dae Gwin,Pareek, Ashwani,Woo, Eui-Jeon,Singh, Prabhjeet,Yo Wiley-Blackwell 2013 Acta crystallographica. Section D, Biological crys Vol.69 No.4
<P>Cyclophilins belong to a family of proteins that bind to the immunosuppressive drug cyclosporin A (CsA). Several members of this protein family catalyze the cis-trans isomerization of peptide bonds preceding prolyl residues. The present study describes the biochemical and structural characteristics of a cytosolic cyclophilin (TaCypA-1) cloned from wheat (Triticum aestivum L.). Purified TaCypA-1 expressed in Escherichia coli showed peptidyl-prolyl cis-trans isomerase activity, which was inhibited by CsA with an inhibition constant of 78.3 nM. The specific activity and catalytic efficiency (kcat/Km) of the purified TaCypA-1 were 99.06 0.13 nmol s(-1) mg(-1) and 2.32 10(5) M(-1) s(-1), respectively. The structures of apo TaCypA-1 and the TaCypA-1-CsA complex were determined at 1.25 and 1.20?? resolution, respectively, using X-ray diffraction. Binding of CsA to the active site of TaCypA-1 did not result in any significant conformational change in the apo TaCypA-1 structure. This is consistent with the crystal structure of the human cyclophilin D-CsA complex reported at 0.96 ? resolution. The TaCypA-1 structure revealed the presence of a divergent loop of seven amino acids (48)KSGKPLH(54) which is a characteristic feature of plant cyclophilins. This study is the first to elucidate the structure of an enzymatically active plant cyclophilin which shows peptidyl-prolyl cis-trans isomerase activity and the presence of a divergent loop.</P>
Defining the copper binding aptamotif and aptamer integrated recovery platform (AIRP)
Sekhon, Simranjeet Singh,Lee, Sang-Hee,Lee, Kyeong-Ah,Min, Jiho,Lee, Byung-Tae,Kim, Kyoung-Woong,Ahn, Ji-Young,Kim, Yang-Hoon Royal Society of Chemistry 2017 Nanoscale Vol.9 No.8
<P>The potential copper binding sites in aptamers have been predicted on the basis of secondary structures and the binding affinity of aptamers with copper. Out of the 4 aptamers (Cu-A1 to Cu-A4) selected by SELEX and examined in the present study, the Cu-A2 aptamer shows the highest binding affinity to copper with the lowest KD value of 1.83 x 10(-11) M. In order to confirm the binding of copper to the proposed region, the binding affinity was experimentally validated using mutation and deletion analysis. We have confirmed that the high G-C pairing patterns and short stem-interval distance play important roles in copper binding. Aptamer specificity was also verified against diverse heavy metals. We also demonstrate an Aptamer Integrated Recovery Platform (AIRP) to recover copper from acidic mine drainage. AIRP can be easily regenerated at least 20 times without significant deterioration of the retrieval performance. To the best of our knowledge, AIRP is the first demonstration of copper specific recovery using aptamers. This can be scaled up and would have diverse applications in metal contaminated water treatment, recovery and as a potential biosensor for environmental analysis, monitoring, and risk assessment.</P>
Toxicoproteomic approaches for analysis of microbial community inhabiting Asian dust particles
Simranjeet Singh Sekhon,Ji-Young Ahn,Jiho Min,Yang-Hoon Kim 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.3
n this review, we provide an overview of the bio-analytical approaches for proteomic analysis of Asian dust storms. Asian dust has been known to travel with high-speed eastward winds and affects air quality over Japan and even the western coast of the U.S. during severe occurrences. Several reports have shown that Asian dust particles have a negative effect on a wide range of industries and human health activities. Here we give a short overview of Asian dust feature and its proteomic analysis approaches including 2-DE PAGE, metaproteomic analysis of large environmental samples, and automatic techniques for dust microbial analysis. Following this, we discuss the detection system of Asian dust particles that can be integrated with biosensor platform.
Sekhon, Simranjeet Singh,Ahn, Ji-Young,Shin, Woo-Ri,Ko, Jung Ho,Lee, Lyon,Dawes, Maisie,Tyler, John,Han, Janet,Kim, Sang Yong,Min, Jiho American Scientific Publishers 2017 Journal of Nanoscience and Nanotechnology Vol.17 No.12
<P>In this study, Pseudozyma jejuensis cutinase is immobilized on amino-functionalized magnetic supports by cross-linked enzyme aggregates (CLEA) for its use in bio-degradation of polycaprolactone (PCL). The amount of cutinase immobilized on the magnetic beads using glutaraldehyde as a coupling agent was measured to be 27 mg/g of beads with 82% recovery of activity after immobilization. Compared to the free enzyme, the immobilized cutinase showed the optimum pH at 1 unit higher (pH 8.0) and also retained its enzymatic activity at higher temperatures. There was 55% retention of cutinase activity after 50 consecutive reuses, indicating its stability and reusability in aqueous media. Moreover, the immobilized cutinase maintained more than 80% of its initial activity during 20 days storage period, while the free cutinase reduced to 48% of initial activity under same condition. In addition, the immobilized cutinase showed a more significant increase in biodegradability over the free cutinase when the immobilized cutinase was used to degrade PCL in a batch system. Higher thermal and storage stability, as well as good durability after repeated use of the immobilized cutinase CLEA, highlights its potential applicability as large scale continuous systems for the enzymatic degradation of PCL.</P>
Sekhon, Simranjeet Singh,Park, Jae-Min,Ahn, Ji-Young,Park, Tae Sun,Kwon, Soon-Dong,Kim, Yoon-Chul,Min, Jiho,Kim, Yang-Hoon 대한독성유전단백체학회 2014 Molecular & cellular toxicology Vol.10 No.2
The para-nitrobenzyl esterase (PNBE) was successfully immobilized onto electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The maximum amount of PNBE immobilized on the PS-PSMA nanofibers was determined to be 56.4 mg/g of nanofibers. The variation of pH and temperature has been observed to affect the enzymatic activity of the free and immobilized PNBE-CLEA. The immobilized PNBE-CLEA has been observed to show improved stability as compared to the native one. PNBE-CLEA has been found to retain more than 63% activity after 60 consecutive reuses. Finally, the immobilized PNBE-CLEA presented a high decomposition efficiency of cephalexin tosylate PNB ester. The durability determination of PNBE-CLEA immobilized nanofibers showed 70% of de-esterification after 10 cycles. These results demonstrate that PNBE-CLEA immobilized nanofiber could be used as an economical and environment friendly system for the catalytic process of para-nitrobenzyl esters in pharmaceutical synthesis.
김선창,Sekhon Simranjeet Singh,Shin Woo-ri,Ahn Gna,조병관,Ahn Ji-Young,김양훈 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1
Background mRNA vaccines hold great potential as therapeutic techniques against viral infections due to their efficacy, safety,and large-scale production. mRNA vaccines offer flexibility in development as any protein can be produced from mRNA without altering the production or application process. Objective This review highlights the iterative optimization of mRNA vaccine structural elements that impact the type,specificity, and intensity of immune responses leading to higher translational potency and intracellular stability. Results Modifying the mRNA structural elements particularly the 5′ cap, 5′-and 3′-untranslated regions (UTRs), the coding region, and polyadenylation tail help reduce the excessive mRNA immunogenicity and consistently improve itsintracellular stability and translational efficiency. Conclusion Further studies regarding mRNA-structural elements and their optimization are needed to create new opportunitiesfor engineering mRNA vaccines.
NGUYENNGOC TU,Simranjeet Singh Sekhon,윤지희,Yang-Hoon Kim,Jiho Min,Y.-H. Kim 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.3
In this study, the effect of different toxic chemicals on Saccharomyces cerevisiae’s vacuoles was determined. More precisely, oxidative stress was mediated by pesticides and heavy metals, whereas nonoxidative stress influence was caused by the treatment with pharmaceuticals. Previously, oxidative stress was reported to alter yeast vacuolar enzymes, thus, intracellular vacuoles are assumed to be used as a biomarker to detect the oxidative stresses on yeast. Simultaneously, nonoxidative stress effect was expected to be recognized by vacuoles as well. The feasibility of toxic detection by vacuolar response was evaluated by confocal microscopy after staining the vacuoles with LysoTracker. The results indicate that each chemical has its own optimal concentration at which the signal corresponding to vacuolar response reached the peak without inhibiting the yeast growth. Therefore, this method was confirmed to detect toxic chemicals at their sub-lethal doses. In addition, the minimum response time of vacuoles for different toxic chemicals was also measured.