2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-Glucoside modulated human umbilical vein endothelial cells injury under oxidative stress

Yan Guo,Wenxue Fan,Shuyu Cao,Yuefeng Xie,Jiancong Hong,Huifen Zhou,Haitong Wan,Bo Jin 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.6

Endothelial cell injury is a major contributor to cardiovascular diseases. The 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-Glucoside (TSG) contributes to alleviate human umbilical vein endothelial cells (HUVECs) injury through mechanisms still know a little. This study aims to clarify the TSG effects on gene expression (mRNA and microRNA) related to oxidative stress and endoplasmic reticulum stress induced by H₂O₂ in HUVECs. We found that TSG significantly reduced the death rate of cells and increased intracellular superoxide dismutase activity. At qRT-PCR, experimental data showed that TSG significantly counteracted the expressions of miR-9-5p, miR-16, miR-21, miR-29b, miR-145-5p, and miR-204-5p. Besides, TSG prevented the expression of ATF6 and CHOP increasing. In contrast, TSG promoted the expression of E2F1. In conclusion, our results point to the obvious protective effect of TSG on HUVECs injury induced by H₂O₂, and the mechanism may through miR16/ATF6/ E2F1 signaling pathway.

Effects of dietary Acremonium terricola culture supplementation on the quality, conventional characteristics, and flavor substances of Hortobágy goose meat

Yawen Guo,Jinyuan Chen,Shuyu Liu,Yali Zhu,Pengfei Gao,Kaizhou Xie 한국축산학회 2022 한국축산학회지 Vol.64 No.5

This study aimed to determine the effect of dietary supplementation with Acremonium terricolaculture (ATC) on the quality, conventional characteristics, and flavor substances of Hortobágygoose meat. A total of 720 one-day-old goslings were divided into four dietary treatments, eachconsisting of six cages of 30 goslings. The dietary conditions consisted of the control group andthree treatment groups supplemented with 3, 5, or 7 g/kg ATC. In male geese, supplementationwith 3 g/kg ATC elevated the crude ash (CA) content of the thigh muscle compared to the controlgroup, and the CA content of the pectoralis major was significantly elevated when geesewere supplemented with 5 g/kg ATC (p < 0.05). In females, compared with the control group,supplementation with 7 g/kg ATC enhanced the crude protein (CP) content of the pectoralismajor. Supplementation with 7 g/kg ATC also increased the crude fat (CF) content of the pectoralismajor in females as well as in both sexes; moreover, this supplementation dose increasedthe inosinic acid content of the thigh muscle in males and in both sexes. In contrast, supplementationwith 5 g/kg ATC decreased the pH of the thigh muscle at 12 h postmortem (p < 0.01). No significant changes in meat color, water loss rate, shear force, moisture content or aminoacid (AA) levels were observed after ATC supplementation (p > 0.05). Levels of saturated fattyacids (SFAs) and polyunsaturated FAs (PUFAs) in the pectoralis major and levels of SFAs,monounsaturated FAs (MUFAs), and PUFAs in the thigh muscle were not affected by the supplementation. Overall, ATC supplementation had positive effects on the pH, and CA, CP, CF,inosinic acid contents as well as on the FA composition of gosling meat. The optimal level ofATC supplementation was 7 g/kg in goslings from 1 to 70 days of age.

Decomposition and Reconstruction of Protein Sequences: The Problem of Uniqueness and Factorizable Langauge

Xiaoli Shi,Bailin Hao,Huimin Xie,Shuyu Zhang 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.1I

In our attempt to justify the CVTree approach of inferring phylogenetic relationship among bacteria from their complete genomes without using sequence alignment, we encountered the problem of the uniqueness of the reconstruction of a protein sequence from its constituent K-peptides, which has a natural relation to a well-understood problem in graph theory, namely, the number of Eulerian loops in a graph. The existence of finite state automata to recognize the uniqueness of a sequence reconstruction provides us with yet another application of factorizable language, which was elucidated at a previous Dynamics Days Asia Pacific meeting (DDAP1).

Pharmacokinetics/Pharmacodynamics models of veterinary antimicrobial agents

Wanhe Luo,Dongmei Chen,Mengru Wu,Zhenxia Li,Yanfei Tao,Qianying Liu,Yuanhu Pan,Wei Qu,Zonghui Yuan,Shuyu Xie 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.5

Misuse and abuse of veterinary antimicrobial agents have led to an alarming increase in bacterial resistance, clinical treatment failure, and drug residues. To address these problems, consistent and appropriate dosage regimens for veterinary antimicrobial agents are needed. Pharmacokinetics/Pharmacodynamics (PK/PD) models have been widely used to establish rational dosage regimens for veterinary antimicrobial agents that can achieve effective prevention and treatment of bacterial diseases and avoid the development of bacterial resistance. This review introduces building methods for PK/PD models and describes current PK/PD research progress toward rational dosage regimens for veterinary antimicrobial agents. Finally, the challenges and prospects of PK/PD models in the design of dosage regimens for veterinary antimicrobial agents are reviewed. This review will help to increase awareness of PK/PD modeling among veterinarians and hopefully promote its development and future use.

Formulation of a rational dosage regimen of ceftiofur hydrochloride oily suspension by pharmacokinetic-pharmacodynamic (PK-PD) model for treatment of swine Streptococcus suis infection

Wanhe Luo,Dehai Wang,Hua Qin,Dongmei Chen,Yuanhu Pan,Wei Qu,Lingli Huang,Shuyu Xie 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6

Background: Our previously prepared ceftiofur (CEF) hydrochloride oily suspension shows potential wide applications for controlling swine Streptococcus suis infections, while the irrational dose has not been formulated. Objectives: The rational dose regimens of CEF oily suspension against S. suis were systematically studied using a pharmacokinetic-pharmacodynamic model method. Methods: The healthy and infected pigs were intramuscularly administered CEF hydrochloride oily suspension at a single dose of 5 mg/kg, and then the plasma and pulmonary epithelial lining fluid (PELF) were collected at different times. The minimum inhibitory concentration (MIC), minimal bactericidal concentration, mutant prevention concentration (MPC), post-antibiotic effect (PAE), and time-killing curves were determined. Subsequently, the area under the curve by the MIC (AUC0–24h/MIC) values of desfuroylceftiofur (DFC) in the PELF was obtained by integrating in vivo pharmacokinetic data of the infected pigs and ex vivo pharmacodynamic data using the sigmoid Emax (Hill) equation. The dose was calculated based on the AUC0–24h/MIC values for bacteriostatic action, bactericidal action, and bacterial elimination. Results: The peak concentration, the area under the concentration-time curve, and the time to peak for PELF's DFC were 24.76 ± 0.92 μg/mL, 811.99 ± 54.70 μg·h/mL, and 8.00 h in healthy pigs, and 33.04 ± 0.99 μg/mL, 735.85 ± 26.20 μg·h/mL, and 8.00 h in infected pigs, respectively. The MIC of PELF's DFC against S. suis strain was 0.25 μg/mL. There was strong concentration-dependent activity as determined by MPC, PAE, and the time-killing curves. The AUC0–24h/MIC values of PELF's DFC for bacteriostatic activity, bactericidal activity, and virtual eradication of bacteria were 6.54 h, 9.69 h, and 11.49 h, respectively. Thus, a dosage regimen of 1.94 mg/kg every 72 h could be sufficient to reach bactericidal activity. Conclusions: A rational dosage regimen was recommended, and it could assist in increasing the treatment effectiveness of CEF hydrochloride oily suspension against S. Suis infections.

Basic Concepts, Recent Advances, and Future Perspectives in the Diagnosis of Bovine Mastitis

Samah Attia Algharib,Ali Sobhy Dawood,Lingli Huang,Aizhen Guo,Gang Zhao,Kaixiang Zhou,Chao Li,Jinhuan Liu,Xin Gao,Wanhe Luo,Shuyu Xie 대한수의학회 2024 Journal of Veterinary Science Vol.25 No.1

Mastitis is one of the most widespread infectious diseases that adversely affects the profitability of the dairy industry worldwide. Accurate diagnosis and identification of pathogens early to cull infected animals and minimize the spread of infection in herds is critical for improving treatment effects and dairy farm welfare. The major pathogens causing mastitis and pathogenesis are assessed first. The most recent and advanced strategies for detecting mastitis, including genomics and proteomics approaches, are then evaluated . Finally, the advantages and disadvantages of each technique, potential research directions, and future perspectives are reported. This review provides a theoretical basis to help veterinarians select the most sensitive, specific, and cost-effective approach for detecting bovine mastitis early.