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RISS 인기검색어
- 검색키워드
- 저자 : Shoffner, R.N. (검색결과 7 건)
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Sara Shoffner,Gi-Yong Koo 글로벌지식마케팅경영학회 2022 Journal of Global Sport Management Vol.7 No.4
Past studies examining sponsorship have primarily focused on thesponsor and not the sporting event. The current study seeks toanalyze how sponsorship associations are interpreted by consum-ers toward the event through the Elaboration LikelihoodAdvertising Model (ELAM). The purpose of this study is to: (1)examine consumers’perceptions of authenticity and unauthentic-ity stemming from sport sponsor-event partnerships and theeffects of each partnership on consumers’elaboration, attitude,and emotion; and (2) assess the impact of consumer attitudesand emotions toward the sporting event on event-related behav-ioral intentions based on perceived authentic and unauthenticpartnerships. Significant differences were found among healthy,unhealthy and CSR experiments for authenticity, elaboration, andattitude but not for emotion. In addition, emotion was positiveand significantly related to event-related behavioral intentions forall three experiments, while attitude was positive and significantlyrelated to event-related behavioral intentions for the healthyexperiment only. Findings provide evidence that sponsorshipassociations affect consumer evaluations toward the event.
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GENE TRANSFER BY MANIPULATION OF PRIMORDIAL GERM CELLS IN THE CHICKEN
Han, Jac Y.,Shoffner, R.N.,Guise, K.S. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.3
The primordial germ cells (PGCs) were transfected in vitro and expressed the exogenous RSVLTR/${\beta}G2$ plasmid, suggesting thaI PGC is a possible vector for direct gene transfer into the germ line. Transfection efficiency of cell suspensions containing PGCs was 1.5% by liposome mediated DNA transfection. By microinjection of the transfected PGCs into the host germinal crescent, PGCs migrated via blood vessel to the future gonad and these transfected PGCs resulted in the RSVLTR/${\beta}G2$ expression in the gonad. The results from the seeding of PGCs on the chorioallantoic membrane were insufficient to test the hypothesis that PGCs can penetrate or invade the chorioallantoic membrane for transport via the circulatory system.
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FREQUENCY OF GIEMSA C-BAND CHROMOSOMES IN THREE INBRED LINES OF CHICKENS
Yeo, J.S.,Shoffner, R.N. Asian Australasian Association of Animal Productio 1989 Animal Bioscience Vol.2 No.1
Giemsa C-banded mitotic chromosome prepatations from White Leghorn, New Hampshire and Rhode Island Red inbred lines were compared for frequency of C-band regions on individual chromosomes. Except for autosomes 3, 6, 8 and 9 and W sex chromosomes, C-banding was extremely variable in other macrochromosomes. No divergence for C-band difference between homologous chromosomes of these lines was detected. Approximately 75% of the mitotic metaphase microchromosomes have recognizable C-band regions with the current technique.
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STABLE TRANSFORMATION OF CULTURED CHICKEN CELLS
Han, J.Y.,Shin, Y.S.,Shoffner, R.N.,Guise, K.S. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.4
A plasmid vector, $RSVLTR/{\beta}G2$, containing lacZ gene under the control of the RSVLTR promoter were transfected into chicken embryo fibroblasts by three different transfection methods. Calcium phosphate, lipsome and DEAE-dextran techniques were applied for transfection of chicken cells. A histochemical assay with X-gal was used as a simple method for screening transfected cells. Plasmid $RSVLTR/{\beta}G2$ was expressed proficiently in the chicken embryo fibroblast. Calcium phosphate-DNA precipitate transfection resulted in the highest efficiency for transient expression of $RSVLTR/{\beta}G2$. Transfected cells formed colonies on the 9th day of incubation indicating stable transformation of the inserted plasmid.
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PRIMORDIAL GERM CELLS IN AVES - Review -
Han, J.Y.,Seo, D.S.,Shoffner, R.N. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.4
Primordial germ cells (PGCs) in aves are the progenitor cells for the gametes. These cells first appear in the epiblast (Eyal-Giladi et al.. 1981). Then translocate and concentrate to endoderm of germinal crescent area in the junction of the area opaca and area pellucida lateral to the primitive streak in stage 4 through 7. They separate from the endoderm, temporarily circulate via the blood vascular system, leave the blood vessels, and finally settle down in the gonadal anlagen at stage 20-24 where they rapidly proliferate to form germ cells. Recently, several attempts have been made to introduce foreign gene into the avian genome to form a transgenic chicken. The stem cells most readily available as vehicles for genetic manipulation of germline in avian species are the PGCs. PGCs have recently been manipulated genetically and used successfully as a vector for gene transfer.
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여정수 ( J . S . Yeo,R . N . Shoffner ) 한국축산학회 1986 한국축산학회지 Vol.28 No.6
Identification of gene locus for domestic animals is the basic step to manipulate gene for the purpose of developing superior individual. Somatic cell hybridization with chicken fibroblast and mouse tumor (Thymidine kinase deficient, TKO cells was available to induce heterokaryote and was found result that normal TK cell included chromosome #7 of chick. Therefore, thymidine kinase gene located at chromosome #7 in chicken.
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닭 초기 배아의 유전자 미세주입과 유전자 발현에 관한 연구
한재용(J . Y . Han),(R . N . Shoffner),(K . S . Guise) 한국축산학회 1994 한국축산학회지 Vol.36 No.3
This study was carried out to examine the expression of marker genes and integration of plasmid DNA into the germ cells in young chicken embryos. The RSVLTR/βG2 plasmid contains the lacZ gene under the control of rous sarcoma virus (RSV) long terminal repeat(LTR) promoter. After a square window of 5㎜ per side was cut in the side of the egg with a dentistry drill, the transfection cocktail of calcium-phosphate or lipofectin with plasmid DNA was microinjected in the area of blastoderm and germinal crescent whose developmental stages were stage X and stage 6 to 8, respectively. Microinjected eggs were sealed, and the eggs were returned to the incubator with the $quot;window$quot; side up overnight. Microinjected embryos with plasmid DNA were screened with Xgal, and the marker gene was expressed in the brain, notochord and other parts of body of 1.5∼4.5 day old embryos, suggesting that developing stem cells in unincubated blastoderms or 1∼4 somite embryos can be transfected with plasmid vectors. The possibility of germ line integration with plasmid DNA by direct microinjection into early chicken embryos was determined. Transfection of stem cells for gonads in the blastoderm or germinal crescent with plasmid vectors was observed. Positive primordial germ cells in the gonad were not observed by plasmid DNA microinjection into unincubated or 24hr incubated embryos in this study. However, the expression of plasmid DNA with RSVLTR promoter in the early chicken embryonic cell shows the possibility of transgenic chicken production by direct microinjection with plasmid DNA. Also genetic manipulation of chicken production traits such as disease resistance, growth and production may he possible in the future.
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