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BMP4 signaling mediates Zeb family in developing mouse tooth.
Shin, Jeong-Oh,Kim, Eun-Jung,Cho, Kyoung-Won,Nakagawa, Eizo,Kwon, Hyuk-Jae,Cho, Sung-Won,Jung, Han-Sung Springer 2012 Histochemistry and cell biology Vol.137 No.6
<P>Tooth morphogenesis is regulated by sequential and reciprocal interaction between oral epithelium and neural-crest-derived ectomesenchyme. The interaction is controlled by various signal molecules such as bone morphogenetic protein (BMP), Hedgehog, fibroblast growth factor (FGF), and Wnt. Zeb family is known as a transcription factor, which is essential for neural development and neural-crest-derived tissues, whereas the role of the Zeb family in tooth development remains unclear. Therefore, this study aimed to investigate the expression profiles of Zeb1 and Zeb2 during craniofacial development focusing on mesenchyme of palate, hair follicle, and tooth germ from E12.5 to E16.5. In addition, we examined the interaction between Zeb family and BMP4 during tooth development. Both Zeb1 and Zeb2 were expressed at mesenchyme of the palate, hair follicle, and tooth germ throughout the stages. In the case of tooth germ at the cap stage, the expression of Zeb1 and Zeb2 was lost in epithelium-separated dental mesenchyme. However, the expression of Zeb1 and Zeb2 in the dental mesenchyme was recovered by Bmp4 signaling via BMP4-soaked bead and tissue recombination. Our results suggest that Zeb1 and Zeb2, which were mediated by BMP4, play an important role in neural-crest-derived craniofacial organ morphogenesis, such as tooth development.</P>
The novel expression of Oct3/4 and Bmi1 in the root development of mouse molars.
Nakagawa, Eizo,Zhang, Li,Shin, Jeong-Oh,Kim, Eun-Jung,Cho, Sung-Won,Ohshima, Hayato,Chen, Zhi,Jung, Han-Sung Springer 2012 Cell and tissue research Vol.347 No.2
<P>The root apex of the tooth elongates until the completion of root development. Although the signaling molecules inducing root elongation have been studied, the characteristic of the cells having the ability to maintain the root elongation remains unclear. This study aimed to investigate the characteristics of the cells involved in the root elongation. Octamer-binding factor 3/4 (Oct3/4) is known as one of the key regulators in maintaining the pluripotency and self-renewal properties of embryonic stem cells. Bmi1, the polycomb-group transcriptional repressor, has emerged as a key regulator in several cellular processes including stem cell self-renewal and cancer cell proliferation. At the beginning of root formation, ameloblasts expressed Oct3/4 in the nucleus, except in the apex of the cervical loop, in which Bmi1and cyclinD were expressed. At PN6, the expression of Oct3/4 in the ameloblasts shifted from the nucleus to the cytoplasm, whereas ameloblastin-negative Hertwig's epithelial root sheath (HERS) cells expressed Bmi1 and cyclinD. By PN10, the cells in the apex of HERS began to express Oct3/4 in their nucleus, whereas Bmi1 and cyclinD began to decrease in their expressions. The odontoblasts consistently expressed Oct3/4 in their cytoplasm. Our results suggest that (1) Oct3/4 creates the border between the ameloblasts from the proliferative region of HERS, (2) Bmi1-positive cells would be one of the candidates resulting in root elongation and (3) the Oct3/4 expression in the cytoplasm of odontoblasts may be related to maintain the odontoblastic characteristics.</P>
miR-200b regulates cell migration via Zeb family during mouse palate development.
Shin, Jeong-Oh,Nakagawa, Eizo,Kim, Eun-Jung,Cho, Kyoung-Won,Lee, Jong-Min,Cho, Sung-Won,Jung, Han-Sung Springer 2012 Histochemistry and cell biology Vol.137 No.4
<P>Palate development requires coordinating proper cellular and molecular events in palatogenesis, including the epithelial-mesenchymal transition (EMT), apoptosis, cell proliferation, and cell migration. Zeb1 and Zeb2 regulate epithelial cadherin (E-cadherin) and EMT during organogenesis. While microRNA 200b (miR-200b) is known to be a negative regulator of Zeb1 and Zeb2 in cancer progression, its regulatory effects on Zeb1 and Zeb2 in palatogenesis have not yet been clarified. The aim of this study is to investigate the relationship between the regulators of palatal development, specifically, miR-200b and the Zeb family. Expression of both Zeb1 and Zeb2 was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium. After contact with the palatal shelves, miR-200b was expressed in the palatal epithelial lining and epithelial island around the fusion region but not in the palatal mesenchyme. The function of miR-200b was examined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of the Zeb family, upregulation of E-cadherin, and changes in cell migration and palatal fusion. These results suggest that miR-200b plays crucial roles in cell migration and palatal fusion by regulating Zeb1 and Zeb2 as a noncoding RNA during palate development.</P>
Simultaneous Determination of Free Capsorubin and Capsanthin in Red Pepper Powder Using u-HPLC
Shim, You-Shin,Kim, Ki-Jin,Seo, Dongwon,Ito, Masahito,Nakagawa, Hiroaki,Arai, Shohei,Ha, Jaeho Oxford University Press 2013 Journal of AOAC International Vol.96 No.2
<B>Abstract</B><P>A simultaneous ultra-HPLC (u-HPLC) method for the determination of free capsorubin and capsanthin in red pepper powder was validated in terms of its precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm, id 2 mm length 100 mm) and with a photodiode-array detector. The recoveries of capsorubin were greater than 83.8 ± 1.7%; the LOD and LOQ of the u-HPLC analyses were 0.043 and 0.129 mg/kg, respectively. The intraday and interday precisions for capsorubin were less than 9.01%. The recoveries of capsanthin were greater than 87.7 ± 1.5%, and the LOD and LOQ were 0.101 and 0.306 mg/kg, respectively. The intraday and interday precisions for capsanthin were less than 12.66%. All calibration curves for capsorubin and capsanthin exhibited good linearity (r2 = 0.99) within the tested ranges.</P>
신문호 ( Moono Shin ),( Minami Nakagawa ),( Akari Sato ) 한국농공학회 2022 한국농공학회 학술대회초록집 Vol.2022 No.-
The basis of Japan’s irrigation management system is a representative, multilayered organization based on local communities, or hamlets (mura) like those of the Edo period, in which farmers can participate in the voting process for land improvement districts. This system allows farmers to mutually coordinate regional conflicts over irrigation, and is a social technology and system that enables the realization of national goals. In recent years, farming patterns have become larger and more corporate, and there is a need to reconstruct an efficient irrigation management system in response to changes in the water management entities of agricultural water use facilities. In particular, labor-saving irrigation management by a small number of farmers is an important issue that is essential in considering the next generation water management system. In this study, a low-cost remote monitoring system using security cameras was installed in a trunk canal at a small land improvement district in Fukushima Prefecture, Japan, and the possibility that the land improvement district in charge of maintenance and management could operate and manage the system was examined. As a result, while the remote monitoring system was found to be effective in saving labor for waterway management, it was considered necessary to consider the daily activities of land improvement district staff when introducing the system. In addition, the running cost required to deal with and maintain the system in case of breakdowns is an important item, and it is considered necessary to devise ways to reduce communication fees in the future.
Shim, You -Shin,Kim, Ki-Jin,Seo, Dongwon,Ito, Masahito,Nakagawa, Hiroaki,Ha, Jaeho Oxford University Press 2012 Journal of AOAC International Vol.95 No.2
<B>Abstract</B><P>A rapid and novel ultra-HPLC (u-HPLC) method for the determination of vitamins A (retinol) and E (α-, γ-, and δ-tocopherol) in foods was validated in terms of its precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm, id 2 mm, and length 75 mm), followed by fluorescence detection. The recovery of retinol was more than 84.58%; the LOD and LOQ of the u-HPLC analysis were 0.015 and 0.045 mg/kg, respectively. The intraday and interday precision was less than 9.12%. The recoveries of α-, γ-, and δ-tocopherol were more than 81.37%; the LOD and the LOQ were 0.014, 0.002, and 0.001 mg/kg and 0.042, 0.005, and 0.004 mg/kg, respectively. All calibration curves had good linearity (r2 = 0.99) within the test ranges. The novel, rapid method coupled to u-HPLC can provide significant improvements in the speed, sensitivity, and resolution compared with a conventional HPLC method.</P>
Shim, You-Shin,Kim, Seunghee,Seo, Dongwon,Ito, Masahito,Nakagawa, Hiroaki,Park, Hyun-Jin,Ha, Jaeho Oxford University Press 2013 Journal of AOAC International Vol.96 No.5
<B>Abstract</B><P>A rapid method for the simultaneous determination of flavonol aglycones in food using ultra-high-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.</P>
Jaeho Ha,You-Shin Shim,Hye-Young Seo,Hyun-Jin Nam,Masahito Ito,Hiroaki Nakagawa 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5
The ultra high performance liquid chromatography (u-HPLC) method for determination of β-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column C18 (particle size 2 μm, i.d. 2 mm, length 50 mm),followed by ultra violet (UV) detection at 450 nm. The recovery of β-carotene was more than 84.4% and the limit of detection and limit of quantitation of u-HPLC analysis were 0.28 and 0.85 μg/mL for β-carotene with butylated hydroxytoluene (BHT) and 0.62 and 1.89 μg/mL for β-carotene without BHT, respectively. The calibration graph for β-carotene was linear from 0.1 to 25.0 μg/mL for u-HPLC. The intra- and interday precisions (relative standard deviations) were <7.5 and <7.8%, respectively. Benefits of u-HPLC analysis of β-carotene in foods is reduction of the analysis time to approximately 1/4, saving the volume of solvent to approximately 1/15. It seems that u-HPLC can offer significant improvements in speed, sensitivity, and resolution compared with conventional HPLC, this bodes well for future applications.