In Vitro Screening for Compounds Derived from Traditional Chinese Medicines with Antiviral Activities Against Porcine Reproductive and Respiratory Syndrome Virus

( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Sheng, Q.K.,Yang, Z.J.,Zhao, H.B.,Wang, X.L.,Guo, J.F. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide ($H_2S$), and 3-methylindole (skatole). Eighty swine ($50.0{\pm}0.5kg$) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a $3{\times}3$ orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, $H_2S$, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, $H_2S$, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced $H_2S$ production (r = 0.9981). Fructan and Bs significantly interacted in $H_2S$ production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and $H_2S$.

Experiment study of structural random loading identification by the inverse pseudo excitation method

Guo, Xing-Lin,Li, Dong-Sheng Techno-Press 2004 Structural Engineering and Mechanics, An Int'l Jou Vol.18 No.6

The inverse pseudo excitation method is used in the identification of random loadings. For structures subjected to stationary random excitations, the power spectral density matrices of such loadings are identified experimentally. The identification is based on the measured acceleration responses and the structural frequency response functions. Numerical simulation is used in the optimal selection of sensor locations. The proposed method has been successfully applied to the loading identification experiments of three structural models, two uniform steel cantilever beams and a four-story plastic glass frame, subjected to uncorrelated or partially correlated random excitations. The identified loadings agree quite well with actual excitations. It is proved that the proposed method is quite accurate and efficient in addition to its ability to alleviate the ill conditioning of the structural frequency response functions.

Research on total resistance of ice-going ship for different floe ice distributions based on virtual mass method

Guo, Wei,Zhao, Qiao-sheng,Tian, Yu-kui,Zhang, Wan-chao The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-

This paper presents the virtual mass method to implement the prediction of total resistance for ice-going ship in floe ice region based on the combined method of CFD and DEM. Two ways of floe ice distribution are adopted for the analysis and comparison. The synthetic ice model test has been conducted to determine the optimal virtual mass coefficients for the two different floe ice distributions. Moreover, the further verification and prediction are developed in different ice conditions. The results show that, the fixed and random distributions in numerical method can simulate the interaction of ship and ice vividly, the trend of total resistance varying with the speed and ice concentration obtained by the numerical simulation is consistent with the model test. The random distribution of floe ice has higher similarity and better accuracy than fixed distribution.

Large-Scale Dataset Incremental Association Rules Mining Model and Optimization Algorithm

Guo Yu-Dong,Li Sheng-Lin,Li Yong-Zhi,Wang Zhao-Xia,Zeng Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.4

Mining association rules is an important research direction in the field of data mining. Related studies have proposed many used to efficiently find large-scale database association rules algorithm, but the research on maintenance problem of association rules is less. Especially many transaction database is always in constant updates. Increase or decrease occurs when the database or dataset minimum support after the change, how to maintain the association rules have been, it got the attention of many researchers. Based on IFP-Growth increment of association rules mining model and to modify the FP-tree, put forward the suitable for transaction data and support the tree model of change, at the same time under different conditions is given incremental association rules mining algorithm, and reduce the frequency of the original dataset range query and query, and in a case of massive dataset multi-level tree structure decomposition, dynamic allocation rule tree branches, ensure load balancing, improve operation efficiency.

Effect of harvest dates on β-carotene content and forage quality of rye (Secale cereale L.) silage and hay

( Guo Qiang Zhao ),( Sheng Nan Wei ),( Chang Liu ),( Hak Jin Kim ),( Jong Geun Kim ) 한국축산학회(구 한국동물자원과학회) 2021 한국축산학회지 Vol.63 No.2

Limited data about the effects of various factors on forage quality and β-carotene content of rye produced in Korea are available, so this study investigated the effects of two preservation methods. Samples were collected from rye harvested every 5 days between April 25 and May 31, and comparisons were done among rye silage wilted for different periods of time and hay of three growth stages of rye. For the silage, dry matter (DM), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents increased with advanced maturity of rye, whereas crude protein, in vitro dry matter digestibility (IVDMD), total digestible nutrients (TDN), relative feed value (RFV), and DM loss decreased (p < 0.0001). Wilting increased the DM content and pH value significantly (p < 0.0001). Silage harvested at the heading stage had the lowest pH value (4.45), propionic acid (0.83 g/kg DM), butyric acid (0 g/kg DM), and fungi and yeast populations (3.70 Log CFU/g of fresh matter [FM]); conversely, it had the highest lactic acid (9.7 g/kg DM), lactic acid bacteria (LAB) (6.87 Log CFU/g of FM), total microorganisms (TM) (7.33 Log CFU/g of FM), and Flieg’s score (70) (p < 0.0001). Wilting elevated LAB and TM populations, but it had no consistent effect on other fermentation products. Both delayed harvest and prolonged wilting decreased β-carotene content. Rye silage harvested around May 9 (heading stage) with 24 h of wilting was preferred for highland, Pyeongchang. For rye hay, advanced maturity decreased DM loss, IVDMD, TDN, and RFV, but it increased DM, ADF, and NDF significantly (p < 0.05). β-carotene was decreased by delay of hay-making. Consequently, to attain lower DM loss and higher hay quality, the harvest date of May 9 (heading stage) is recommended.

Structural and nanomechanical properties of a-plane ZnO thin films deposited under different oxygen partial pressures

Sheng-Rui Jian,Hou-Guang Chen,Guo-Ju Chen,Jason S.C. Jang,Jenh-Yih Juang 한국물리학회 2012 Current Applied Physics Vol.12 No.3

The effects of O2 partial pressure during RF magnetron sputtering on the structural and nanomechanical properties of a-plane ZnO thin films were investigated by using X-ray diffraction (XRD) and nanoindentation techniques. The XRD and the transmission electron microscopy (TEM) selected area diffraction results indicate that the epitaxial relationship between ZnO thin films and Al2O3 substrates is ZnO ð1120Þ//Al2O3 ð1102Þ. The average values of the hardness and Young’s modulus of the a-plane ZnO films were found to decrease with increasing oxygen partial pressure. The cross-sectional TEM revealing the localized plastic deformation of ZnO thin films beneath the Berkovich indenter, indicating the prominent role played by the threading dislocations in the film deformation behavior. At higher indentation loadings, the sapphire substrate exhibits extensive deformation with narrow slip bands appearing on {0001} plane. However, no evidence of pressure-induced phase transformation, as well as cracking and/or delamination phenomena at the filmesubstrate interface was observed.

Variation of Urinary and Serum Trace Elements (Ca, Zn, Cu, Se) in Bladder Carcinoma in China

Guo, Kun-Feng,Zhang, Zhe,Wang, Jun-Yong,Gao, Sheng-Lin,Liu, Jiao,Zhan, Bo,Chen, Zhi-Peng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Backgrounds: Deficiency or excess of trace elements can induce body metabolic disorders and cellular growth disturbance, even mutation and cancerization. Since there are few studies of the effect of trace elements in bladder carcinoma in China, the aim of this study was thus to assess variation using a case control approach. Methods: To determine this, 81 patients with bladder carcinoma chosen as a study group and 130 healthy persons chosen as a control group were all assayed for urinary and serum trace elements (calcium [Ca], zinc [Zn], copper [Cu], selenium [Se]) using an atomic absorption spectrophotometer, and the results were analyzed by independent sample t tests. The correlative factors on questionnaires answered by all persons were analyzed by logistic regression. Results: The results showed urinary Ca, Zn and serum Cu levels of the study group to be significantly higher (P<0.05) than those of he control group. Serum Ca and Se levels of study group were significantly lower (P<0.05) than those of control group. Conclusion: There were higher urinary Zn and serum Cu concentrations in bladder carcinoma cases. Bladder carcinoma may be associated with Ca metabolic disorder, leading to higher urinary Ca and lower serum Ca. Low serum Se and smoking appear to be other risk factors for bladder carcinoma in China.

Somatostatin Receptors 3, 4 and 5 Play Important Roles in Gallbladder Cancer

Guo, Run-Sheng,Shi, Pei-Dong,Zhou, Jie,Chen, Yue-Yu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Expression changes of somatostatin receptor subtypes (SSTRs) including SSTR1, SSTR2, SSTR3, SSTR4 and SSTR5 in the development of gallbladder cancer were assessed with attention to relationships with clinical pathological characteristics. SSTRs in 29 gallbladder cancer and 25 normal gallbladder tissue specimens were examined by immunohistochemical staining. Differences between SSTRs expressions and clinical pathological parameters were analyzed by chi-square test. The five subtypes of SSTR were all expressed in gallbladder cancer tissues and SSTR3 presented the highest expression. SSTR5 expression was increased significantly in gallbladder cancer (P<0.05) compared with that in normal gallbladder tissue. SSTR3 expression in highly and moderately differentiated gallbladder cancer was significantly higher than that in poorly differentiated lesions (P<0.05). SSTR4 expression was lower in gallbladder cancer with lymph node metastasis than that in gallbladder cancer without lymph node metastasis (P<0.05). Therfore, these results indicated that SSRT5, SSTR3 and SSTR4 may play important roles in the formation and development of gallbladder cancer.

The microstructure evolution and mechanical properties of ammonium polyphosphate/ aluminium hydroxide/ mica during thermal reaction

Sheng HU,Fei Chen,Jun-Guo Li,Qiang Shen,Zhi-Xiong Huang,Lian-Meng Zhang 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.8

The phase and microstructure evolution of ammonium polyphosphate/ aluminium hydroxide/ mica compounds (AAM) duringthermal reaction are investigated. At 300 oC, AAM residues exhibit porous structure due to gas evolution from the thermaldecomposition of ammonium polyphosphate (APP) and aluminium hydroxide, but mica does not react with them, and theflexural strength of specimens can reach 11.56 MPa. The thermal decomposition products of APP react with AlOOH toNH4AlP2O7, which can improve the flexural strength during 300 ~ 400 oC. The reactivity of mica is increased by eliminatinghydroxyl at temperature up to 600 oC, and the flexural strength of AAM residues is improved furtherly by the chemicallyinteractions between active mica and phosphates during 600 ~ 1000 oC. And specifically at 1000 oC, the AAM residues exhibitrelatively high flexural strength, which can reach 26.85 MPa and reactions between mica and KAlP2O7 could generateKAlSi3O8 and 3Al2O3·2SiO2. AAM with high flexural strength from low to high temperatures is expected to be a kind ofexcellent inorganic additive of ceramifiable polymer with wide temperature range for using.