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- 저자 : Shekhar B. Jadhav (검색결과 2 건)
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Saha, Shouvik,Jeon, Byong-Hun,Kurade, Mayur B.,Jadhav, Shekhar B.,Chatterjee, Pradip K.,Chang, Soon Woong,Govindwar, Sanjay Prabhu,Kim, Sun Joon Elsevier 2018 Journal of cleaner production Vol.190 No.-
<P><B>Abstract</B></P> <P>A proper waste management practice such as anaerobic digestion for the waste generated by the agro-food industries could minimize the amount of material disposal to landfill. In our study, the improvement of methane production was elucidated through the pretreatment optimization of the mixed fruit wastes (FW). Dilute acetic acid pretreatment of FW was optimized in order to increase the bioavailability and microbial accessibility. A maximum sugar recovery of 95% was achieved from the pretreated FW under the optimized conditions (0.2 M acetic acid, 62.5 °C, and 30 min). Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric (TG) analyses verified the presence of cellulosic material in the pretreated FW. X-ray diffraction (XRD) analysis indicated that the crystallinity index was increased to 56% after the disruption of complex hemicellulosic structures during pretreatment. Increased porosity and surface roughness of pretreated FW for better microbial attachment were confirmed in scanning electron microscopy (SEM). Anaerobic digestion showed increased methanogenic activity (10.17 mL g<SUP>−1</SUP> VS<SUB>initial</SUB> d<SUP>−1</SUP>) in pretreated FW, during 86-day experimental period due to better microbial attachment and accessibility during the digestion process. Higher methane yield of 53.58 mL g<SUP>−1</SUP> VS<SUB>initial</SUB> was observed in pretreated FW. Thus, acetic acid pretreatment is an effective method to improve the utilization and conversion of FW to methane.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fruit waste pretreatment was optimized by employing RSM. </LI> <LI> Under optimized conditions, pretreatment recovered 95% of the total sugar. </LI> <LI> Optimized pretreatment improved methane yield by 10%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Dhawal P. Tamboli,Amar A. Telke,Vishal V. Dawkar,Shekhar B. Jadhav,Sanjay P. Govindwar 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4
Aryl alcohol oxidase (AAO) produced by dye decolorizing bacteria Sphingobacterium sp. ATM, was purified 22.63 fold to a specific activity of 21.75 μmol/min/mg protein using anion exchange and size exclusion chromatography. The molecular weight of the purified AAO was found to be 71 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE),and confirmed by zymography of AAO using L-dopa. The enzyme showed substrate specificity towards veratryl alcohol, followed by n-propanol. The optimum pH and temperature of purified AAO were found to be 3.0 and 40°C, respectively. The K_m and V_(max) of AAO was 1.1615mM and 3.13 mM/min when veratryl alcohol was used as substrate. Sodium azide showed maximum inhibition while ethylenediamine tetra acetic acid (EDTA), L-cysteine and dithiothreitol showed slight inhibition. Metal ions also showed slight inhibition. HPLC analysis confirmed the degradation of Direct Red 5B. The metabolite obtained after decolorization of Direct Red 5B was characterized as 3 diazenyl 7 [-(phenyl carbonyl) amino] naphthalene-2-sulfonic acid using GC-MS analysis.
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