http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
MicroRNAs in Autoimmune Sjögren's Syndrome
Cha, Seunghee,Mona, Mahmoud,Lee, Kyung Eun,Kim, Dong Hee,Han, Kyudong Korea Genome Organization 2018 Genomics & informatics Vol.16 No.4
MicroRNAs (miRNAs), small non-coding RNAs, have been implicated in various diseases and cellular functions as microregulators of gene expression. Although the history of miRNA investigation in autoimmune $Sj{\ddot{o}}gren^{\prime}s$ syndrome (SjS) is fairly short, a substantial amount of data has already been accumulated. These findings clearly indicate potential clinical implications of miRNAs, such as autoantigen expression and autoantibody production, viral miRNAs regulating the calcium signaling pathway, and aberrant immune cell regulation and cytokine production. Research endeavors in the field are currently underway to select disease-specific diagnostic and prognostic biomarkers by utilizing different types of tissues or biological specimens of SjS patients. Various techniques for miRNA analysis with different stringencies have been applied, with the most recent one being next-generation sequencing. This review compiles and highlights differentially-expressed miRNAs in various samples collected from SjS patients and their potential implications in the pathogenesis of SjS. To facilitate the development of miRNA-targeted personalized therapy in the future, we urge more follow-up studies that confirm these findings and elucidate the immunopathological roles of differentially-expressed miRNAs. Furthermore, improved diagnostic criteria for the disease itself will minimize sampling errors in patient recruitment, preventing the generation of inconsistent data.
Bae, Seunghee,Ha, Tae-Su,Yoon, Youngmin,Lee, Joonyoung,Cha, Hwa Jun,Yoo, Hoesook,Choe, Tae-Boo,Li, Shunhua,Sohn, Insook,Kim, Ji-Young,Kim, Cha-Soon,Jin, Hyeon-Ok,Lee, Hyung-Chahn,Park, In-Chul,Kim, Ch D.A. Spandidos 2008 International journal of molecular medicine Vol.21 No.3
<P>Apoptosis executed by the mammalian caspase family plays a fundamental role in cellular homeostasis. Deregulation of this process is associated with several human diseases. The multimerization of ligand-induced death receptors results in the recruitment of the death inducing signaling complex and autocatalytic activation of initiator caspases, including caspase-8 and -10. However, it is still unclear how initiator caspases trigger and control the early apoptotic signaling pathways, partly because the downstream proteolytic cleavage targets of the initiator caspases are not completely known. Although it is known that a number of proteins are cleaved by various members of the caspase family, the identification of specific cleavage substrates of the initiator caspases 8 and 10, has been hindered by a lack of systematic and broadly applicable strategies for substrate identification. In the present study we constructed a mouse cDNA library and used it to perform a systematic, genome-wide screen for novel in vitro substrates of caspase-8 and -10. From this, we successfully identified six putative caspase substrates, including five novel proteins (ABCF1, AKAP1, CPE, DOPEY1 and GOPC1) that may be targeted specifically by the initiator caspases 8 and 10 during the early stages of apoptosis. These findings may provide useful information for elucidating the apoptotic signaling pathways downstream of the death receptors.</P>
Paroxetine-induced Hypoglycemia in Type 2 Diabetic Patient
Seunghee Han,Hye-Sun Park,Yong-ho Lee,Byung-Wan Lee,Eun Seok Kang,Bong-Soo Cha 이화여자대학교 의과학연구소 2016 EMJ (Ewha medical journal) Vol.39 No.1
Selective serotonin reuptake inhibitors are commonly prescribed drugs for the treatment of depression in the patients with diabetes. Here, we report a case of paroxetineinduced severe recurrent hypoglycemia that developed in a 35-year-old woman with poorly controlled type 2 diabetes complicated by diabetic nephropathy and neuropathy. She discontinued her daily insulin therapy 2 months after the introduction of paroxetine, but hypoglycemic events were sustained. After discontinuation of paroxetine, no more hypoglycemic events occurred.
Identification of ultraviolet B radiation-induced microRNAs in normal human dermal papilla cells
CHA, HWA JUN,KIM, OK-YEON,LEE, GANG TAI,LEE, KWANG SIK,LEE, JAE HO,PARK, IN-CHUL,LEE, SU-JAE,KIM, YU RI,AHN, KYU JOONG,AN, IN-SOOK,AN, SUNGKWAN,BAE, SEUNGHEE SPANDIDOS PUBLICATIONS 2014 MOLECULAR MEDICINE REPORTS Vol.10 No.4
<P>Ultraviolet (UV) radiation impairs intracellular functions by directly damaging DNA and by indirectly generating reactive oxygen species (ROS), which induce cell cycle arrest and apoptosis. UV radiation can also alter gene expression profiles, including those of mRNA and microRNA (miRNA). The effects of UV radiation on cellular functions and gene expression have been widely documented in human skin cells such as keratinocytes, melanocytes and dermal fibroblasts, but the effect it has on other types of skin cell such as dermal papilla cells, which are crucial in the induction of hair follicle growth, remains unknown. In the current study, the effect of UV radiation on physiological changes and miRNA-based expression profiles in normal human dermal papilla cells (nHDPs) was investigated. UVB radiation of ≥50 mJ/cm<SUP>2</SUP> displayed high cytotoxicity and apoptosis in a dose-dependent manner. In addition, ROS generation was exhibited in UVB-irradiated nHDPs. Furthermore, using miRNA microarray analysis, it was demonstrated that the expression profiles of 42 miRNAs in UVB-irradiated nHDPs were significantly altered compared with those in the controls (35 upregulated and 7 downregulated). The biological functions of the differentially expressed miRNAs were studied with gene ontology analysis to identify their putative target mRNAs, and were demonstrated to be involved in cell survival- and death-related functions. Overall, the results of the present study provide evidence that miRNA-based cellular mechanisms may be involved in the UVB-induced cellular response in nHDPs.</P>
Overdosage of Methylparaben Induces Cellular Senescence In Vitro and In Vivo
Cha, Hwa Jun,Bae, Seunghee,Kim, Karam,Kwon, Seung Bin,An, In-Sook,Ahn, Kyu Joong,Ryu, Junghwa,Kim, Hey-Sun,Ye, Sang-Kyu,Kim, Byung-Hak,An, Sungkwan The Society for Investigative Dermatology, Inc 2015 The Journal of investigative dermatology Vol.135 No.2
구조 건전성 모니터링을 위한 Terrestrial LiDAR기반 형상변화 검출 알고리즘
차기춘 ( Cha Gichun ),마루마이클베케레 ( Maru Michael Bekele ),징슈주 ( Jing Shuju ),박승희 ( Park Seunghee ) 한국구조물진단유지관리공학회 2022 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.26 No.2
본 연구에서는 구조물의 외부형상 변화를 확인할 수 있는 지상 라이다와 컴퓨터비전 기술을 활용한 형상변화 검출 알고리즘을 개발한다. 형상변화 검출 알고리즘은 구조물의 미세변형을 데이터의 연관성과 계층적 구조로 융합된 3차원 형상데이터로 시각화하여 변형 지점을 확인한다. 이를 통해 구조물의 취약한 부분과 위험한 부분까지도 확인할 수 있는 구조 건전성 모니터링 및 구조물 안전점검 기술로 활용될 수 있기를 기대한다.
Bae, Seunghee,Kim, Karam,Cha, Hwa Jun,Choi, Yeongmin,Shin, Shang Hun,An, In-Sook,Lee, Jae Ho,Song, Jie-Young,Yang, Kwang Hee,Nam, Seon Young,An, Sungkwan Lychnia 2014 International journal of oncology Vol.45 No.4
<P>The polycomb group RING finger protein, B-cell?specific moloney murine leukemia virus integration site?1 (BMI1), has emerged as a key regulator of cell proliferation, cell cycle, cell immortalization, chemoresistance and radioresistance. Although the radioresistant effect of BMI1 has been thoroughly investigated, the effectiveness of this factor on low-dose radiation (LDR) resistance has not been explored. Here, we demonstrate that BMI1 is not critical for altering cell viability or cell growth in response to LDR, but BMI1 changes cellular gene expression profiles in response to LDR. Normal human dermal fibroblasts (NHDFs) stably expressing BMI1 short hairpin RNA (shRNA) did not exhibit changes in cell viability or cell cycle distribution assays following exposure to 0.1?Gy of γ-radiation. However, microRNA (miRNA) microarrays revealed that a lack of BMI1 leads to changes in miRNA expression in response to LDR. Bioinformatics analyses demonstrated that predicted target genes of the altered miRNAs are functionally involved in both negative and positive regulation of cell growth, cell proliferation, cell cycle and apoptosis. Therefore, these results indicate that low radiosensitivity even in the absence of the radioresistant factor BMI1 is related with the altered miRNA expression profiles in NHDF.</P>
Resveratrol alters microRNA expression profiles in A549 human non-small cell lung cancer cells
Bae, Seunghee,Lee, Eun-Mee,Cha, Hwa Jun,Kim, Karam,Yoon, Yeongmin,Lee, Hyunjin,Kim, Jongran,Kim, Yu-Jeong,Lee, Hong Ghi,Jeung, Hoi-Kyung,Min, Yoo Hong,An, Sungkwan Springer-Verlag 2011 Molecules and cells Vol.32 No.3
포인트 클라우드 데이터를 이용한 OSP기반 교량 구조물 디지털 형상관리
차기춘 ( Cha Gichun ),박승희 ( Park Seunghee ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.1
본 연구에서는 포인트 클라우드 데이터를 이용하여 교량 구조물의 디지털 형상관리가 가능한 데이터베이스 시스템을 제안한다. 교량 형상관리는 포인트 클라우드 데이터에 공간과 부피를 부여하여, Voxel 기반의 3D모델을 생성하고, Voxel간 위치 관계성을 부여할 수 있는 OSP 기법을 적용하여, 교량 형상의 마지막 단에 위치한 Leaf Node의 변화량을 추정한다. 이를 통해 교량 디지털 형상데이터를 확보하고, 향후 인공지능 모델기반의 구조물 유지관리 기술로 발전하기를 기대한다.