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Efficient Adhesion-Based Plasma Membrane Isolation for Cell Surface <i>N</i>-Glycan Analysis
Mun, Ji-Young,Lee, Kyung Jin,Seo, Hoon,Sung, Min-Sun,Cho, Yee Sook,Lee, Seung-Goo,Kwon, Ohsuk,Oh, Doo-Byoung American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.15
<P>Glycans, which decorate cell surfaces, play crucial roles in various physiological events involving cell surface recognition. Despite the importance of surface glycans, most analyses have been performed using total cells or whole membranes rather than plasma membranes due to difficulties related to isolation. In the present study, we employed an adhesion-based method for plasma membrane isolation to analyze <I>N</I>-glycans on cell surfaces. Cells were attached to polylysine-coated glass plates and then ruptured by hypotonic pressure. After washing to remove intracellular organelles, only a plasma membrane fraction remained attached to the plates, as confirmed by fluorescence imaging using organelle-specific probes. The plate was directly treated with trypsin to digest and detach the glycoproteins from the plasma membrane. From the resulting glycopeptides, <I>N</I>-glycans were released and analyzed using MALDI-TOF mass spectrometry and HPLC. When <I>N</I>-glycan profiles obtained by this method were compared to those by other methods, the amount of high-mannose type glycans mainly contaminated from the endoplasmic reticulum was dramatically reduced, which enabled the efficient detection of complex type glycans present on the cell surface. Moreover, this method was successfully used to analyze the increase of high-mannose glycans on the surface as induced by a mannosidase inhibitor treatment.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-15/ac401431u/production/images/medium/ac-2013-01431u_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac401431u'>ACS Electronic Supporting Info</A></P>
Deactivation characteristics of Ni and Ru catalysts in tar steam reforming
Park, Seo Yun,Oh, Gunung,Kim, Kwangyul,Seo, Myung Won,Ra, Ho Won,Mun, Tae Young,Lee, Jae Goo,Yoon, Sang Jun Elsevier 2017 RENEWABLE ENERGY Vol.105 No.-
<P><B>Abstract</B></P> <P>Tar formation resulting during lignocellulosic biomass gasification is a major impediment to utilizing biomass energy sources, in that it blocks and fouls the processing equipment; as such, any tar present in the produced syngas much be effectively removed. This study analyzes the ability of commercially available Ni and Ru based CH<SUB>4</SUB> reforming catalysts to effect tar removal and compares deactivation characteristics. Toluene was used as the model biomass tar at concentrations of 30 and 100 g/Nm<SUP>3</SUP>. Several additional parameters were also tested, including reaction temperatures (400–800 °C), space velocities (5000–30,000 h<SUP>−1</SUP>), and the steam/toluene ratios (2–20). The variation of toluene conversion and product gas composition with reaction conditions was analyzed. Overall, H<SUB>2</SUB> and CO production were favored by the Ru catalyst and generally increased with temperature. Conversion also increased with temperature, with conversions higher than 90% obtained at 800 °C.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ru-containing RUA and Ni-containing FCR-4 were tested as tar reforming catalysts. </LI> <LI> Toluene was used as the model tar at both standard and high concentrations. </LI> <LI> Both catalysts effected over 90% conversion at 800 °C for standard toluene loading. </LI> <LI> Conversion increased with steam content and decreased as space velocity increased. </LI> <LI> FCR-4 showed significantly greater coke deposition, accounting for deactivation. </LI> </UL> </P>
Jisu Kim,Goo-Hyun Mun,Sung Wook Seo,Kyeong-Tae Lee 대한수부외과학회 2022 대한수부외과학회지 Vol.27 No.4
Purpose: Wide excision and subsequent reconstruction of the defect are crucial pillars in the treatment of soft tissue sarcoma (STS); however, those procedures carry a relatively high risk of postoperative complications, which could raise oncologic concerns. The present study evaluated the association of postoperative complications after resection and immediate reconstruction with STS recurrence. Methods: We reviewed patients with primary STS who underwent wide resection and immediate reconstruction between 2011 and 2019. Patients were categorized into three groups based on their postoperative inflammatory complication status: no complications, noninflammatory complications, and inflammatory complications. Inflammatory complications were defined as those involving a sustained elevation of inflammatory markers in laboratory tests after postoperative 2 weeks. The cumulative incidence of oncologic events and their respective hazard ratios (HRs) were evaluated using multivariable Cox regression analyses. Results: In total, 94 patients with a median follow-up of 54.8 months were analyzed, including 17 with inflammatory complications, 17 with noninflammatory complications, and 60 with no complications. The three groups showed similar baseline characteristics except for older age and a lower rate of FNCLCC (Fédération Nationale des Centres de Lutte Contre Le Cancer) grade 3 in the inflammatory complications group. The inflammatory complications group showed significantly worse disease-free survival than the no complications group. This difference remained significant after adjustment for other variables in multivariate analyses (HR, 3.485; p=0.019). The development of noninflammatory complications was not associated with oncologic outcomes. Conclusion: Our findings suggest that the development of inflammatory complications following wide excision and immediate reconstruction may be associated with the recurrence of STS.
서명원(Myung Won Seo),라호원(Ho Won Ra),윤상준(Sang Jun Yoon),문태영(Tae-Young Mun),문지홍(Ji Hong Moon),윤성민(Sung Min Yoon),박성진(Sung Jin Park),김재호(Jae Ho Kim),김용구(Yong Ku Kim),이재구(Jae Goo Lee) 한국열환경공학회 2020 한국열환경공학회 학술대회지 Vol.2020 No.춘계
최근 세계 각국에서 기후변화 및 환경오염 문제를 해결하고 이를 경제사회의 구조변화와 연계하는 “그린 뉴딜”정책들이 많이 시도되고 있으며, 특히 유럽은 유럽 그린딜 (European Green Deal)이란 이름으로 2050년까지 유럽을 탄소 중립 지역으로 만들고, 순환 경제를 통한 폐기물 저감에도 많은 노력을 쏟고 있다. 바이오매스 및 폐기물 가스화 기술은 탄소 중립인 바이오매스와 순환경제에서 처리가 되어야 하는 종말 폐기물을 보다 친환경적으로 처리할 수 있는 기술로서 생성된 합성가스는 전환 공정을 통하여 수소, 메탄올, 디젤 등을 생산할 수 있는 플랫폼 기술이다. 한국에너지기술연구원에서는 지난 21년간 다양한 폐기물/바이오매스 연료를 대상으로 고정층, 유동층, 플라즈마 방식 등의 다양한 가스화 기술개발을 수행하였다. 본 연구에서는 과거 프로젝트 및 현재 진행 중인 미이용 바이오매스 및 폐플라스틱 가스화기 개발 연구를 중점적으로 소개하고 향후 국내외 가스화 기술 개발 전망을 제시하고자 한다. (국문 또는 영문, 200단어 이내, 10pt, 줄간격 160%, 휴면명조)